The Effect of Lemongrass Leaves and Stalks Extracts using Methanol as The Eco-friendly Larvicides on Fourth Instar Aedes aegypti Larvae

Authors

  • Tjut Mariam Department of Parasitology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia https://orcid.org/0000-0002-1392-200X
  • Ade Oktiviyari Department of Oral Biology, Faculty of Dentistry, Universitas Syiah Kuala, Banda Aceh, Indonesia https://orcid.org/0000-0002-1392-200X
  • Ahmad Yamin Harahap Department of Parasitology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6727

Keywords:

Aedes aegypti, Cymbopogon citratus, Larvacide, Insecticides, Citronella

Abstract

BACKGROUND: Aedes aegypti is one of the most vicious mosquitoes, known for its role in several deadly diseases, including dengue fever and Zika. Several strategies have been developed over decades to prevent vector-borne diseases; one of them is insecticide to control the mosquito population. However, this strategy would not last long due to the elevation of resistance, environmental problem, and some issues regarding human health. Natural products have become major options to combat the glitches. Lemongrass (Cymbopogon citratus) has known for its toxic effect on mosquito larva.

AIM: The objective of this study was to assess the effect of C. citratus extract against fourth instar of A. aegypti larvae.

METHODS: Three extract concentrations, 2.5%, 5%, and 7.5%, were used. The larvicide activity was evaluated at 2, 4, and 6 h. Our data indicated that all concentrations contributed to increase larvae mortality.

RESULTS: Lethal Concentration (LC) 50 and LC90 were achieved at 2.5% and 4.1% of extract, respectively.

CONCLUSION: C. citratus  extract has a larvicide activity against fourth instar of A. aegypti.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Hales S, de Wet N, Maindonald J, Woodward A. Potential effect of population and climate changes on global distribution of dengue fever: An empirical model. Lancet. 2002;360(9336):830-4. https://doi.org/10.1016/s0140-6736(02)09964-6 PMid:12243917 DOI: https://doi.org/10.1016/S0140-6736(02)09964-6

Chen R, Vasilakis N. Dengue--quo tu et quo vadis? Viruses. 2011;3(9):1562-608. https://doi.org/10.3390/v3091562 PMid:21994796 DOI: https://doi.org/10.3390/v3091562

Khetarpal N, Khanna I. Dengue fever: Causes, complications, and vaccine strategies. J Immunol Res. 2016;2016:6803098. https://doi.org/10.1155/2016/6803098 PMid:27525287 DOI: https://doi.org/10.1155/2016/6803098

Yang YC, Lee SG, Lee HK, Kim MK, Lee SH, Lee HS. A piperidine amide extracted from Piper longum L. fruit shows activity against Aedes aegypti mosquito larvae. J Agric Food Chem. 2002;50(13):3765-7. https://doi.org/10.1021/jf011708f PMid:12059157 DOI: https://doi.org/10.1021/jf011708f

Senthil-Nathan S. A review of resistance mechanisms of synthetic insecticides and botanicals, phytochemicals, and essential oils as alternative larvicidal agents against mosquitoes. Front Physiol. 2020;10:1591. https://doi.org/10.3389/fphys.2019.01591 PMid:32158396 DOI: https://doi.org/10.3389/fphys.2019.01591

Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar AN, et al. Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms. PLoS One. 2020;15(5):e0232172. https://doi.org/10.1371/journal.pone.0232172 PMid:32365106 DOI: https://doi.org/10.1371/journal.pone.0232172

Bahlai CA, Xue Y, McCreary CM, Schaafsma AW, Hallett RH. Choosing organic pesticides over synthetic pesticides may not effectively mitigate environmental risk in soybeans. PLoS One. 2010;5(6):e11250. https://doi.org/10.1371/journal.pone.0011250 PMid:20582315 DOI: https://doi.org/10.1371/journal.pone.0011250

Choochote W, Chaithong U, Kamsuk K, Jitpakdi A, Tippawangkosol P, Tuetun B, et al. Repellent activity of selected essential oils against Aedes aegypti. Fitoterapia. 2007;78(5):359-64. https://doi.org/10.1016/j.fitote.2007.02.006 PMid:17512681 DOI: https://doi.org/10.1016/j.fitote.2007.02.006

Gokulakrishnan J, Kuppusamy E, Shanmugam D, Appavu A, Kaliyamoorthi K. Pupicidal and repellent activities of Pogostemon cablin essential oil chemical compounds against medically important human vector mosquitoes. Asian Pac J Trop Dis. 2013;3(1):26-31. https://doi.org/10.1016/s2222-1808(13)60006-7 DOI: https://doi.org/10.1016/S2222-1808(13)60006-7

Nerio LS, Olivero-Verbel J, Stashenko E. Repellent activity of essential oils: A review. Bioresour Technol. 2010;101(1):372-8. https://doi.org/10.1016/j.biortech.2009.07.048 PMid:19729299 DOI: https://doi.org/10.1016/j.biortech.2009.07.048

Phasomkusolsil S, Soonwera M. Insect repellent activity of medicinal plant oils against Aedes aegypti (Linn.), Anopheles minimus (Theobald) and Culex quinquefasciatus Say based on protection time and biting rate. Southeast Asian J Trop Med Public Health. 2010;41(4):831-40. https://doi.org/10.1016/s2222-1808(13)60069-9 PMid:21073057 DOI: https://doi.org/10.1016/S2222-1808(13)60069-9

Bossou AD, Mangelinckx S, Yedomonhan H, Boko PM, Akogbeto MC, De Kimpe N, et al. Chemical composition and insecticidal activity of plant essential oils from Benin against Anopheles gambiae (Giles). Parasit Vectors. 2013;6:337. https://doi.org/10.1186/1756-3305-6-337 PMid:24298981 DOI: https://doi.org/10.1186/1756-3305-6-337

Sukumar K, Perich MJ, Boobar LR. Botanical derivatives in mosquito control: A review. J Am Mosq Control Assoc. 1991;7(2):210-37. PMid:1680152

Soonwera M, Phasomkusolsil S. Effect of Cymbopogon citratus (lemongrass) and Syzygium aromaticum (clove) oils on the morphology and mortality of Aedes aegypti and Anopheles dirus larvae. Parasitol Res. 2016;115(4):1691-703. https://doi.org/10.1007/s00436-016-4910-z PMid:26796022 DOI: https://doi.org/10.1007/s00436-016-4910-z

Hsu WS, Yen JH, Wang YS. Formulas of components of citronella oil against mosquitoes (Aedes aegypti). J Environ Sci Health B. 2013;48(11):1014-9. https://doi.org/10.1080/03601234.2013.8166 13 PMid:23998314 DOI: https://doi.org/10.1080/03601234.2013.816613

Senthilkumar N, Varma P, Gurusubramanian G. Larvicidal and adulticidal activities of some medicinal plants against the malarial vector, Anopheles stephensi (Liston). Parasitol Res. 2009;104(2):237-44. https://doi.org/10.1007/s00436-008-1180-4 PMid:18787842 DOI: https://doi.org/10.1007/s00436-008-1180-4

Cavalcanti ES, de Morais SM, Lima MA, Santana EW. Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Mem Inst Oswaldo Cruz. 2004;99(5):541-4. https://doi.org/10.1590/s0074-02762004000500015 PMid:15543421 DOI: https://doi.org/10.1590/S0074-02762004000500015

Downloads

Published

2021-09-11

How to Cite

1.
Mariam T, Oktiviyari A, Yamin Harahap A. The Effect of Lemongrass Leaves and Stalks Extracts using Methanol as The Eco-friendly Larvicides on Fourth Instar Aedes aegypti Larvae. Open Access Maced J Med Sci [Internet]. 2021 Sep. 11 [cited 2024 Apr. 26];9(B):937-9. Available from: https://oamjms.eu/index.php/mjms/article/view/6727

Issue

Section

Infective Diseases

Categories