In vitro Antifungal Activity of Extracts of Moringa oleifera on Phytopathogenic Fungi Affecting Carica papaya

Margaret Oniha; Angela Eni; Olayemi Akinnola; Emmanuel Adedayo Omonigbehin; Eze Frank Ahuekwe; John Folashade Olorunshola

doi:10.3889/oamjms.2021.6794

Authors

Margaret Oniha Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria https://orcid.org/0000-0001-5757-8370
Angela Eni Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria https://orcid.org/0000-0001-5072-6086
Olayemi Akinnola Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
Emmanuel Adedayo Omonigbehin Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria
Eze Frank Ahuekwe Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria https://orcid.org/0000-0003-1477-4050
John Folashade Olorunshola Department of Biological Sciences, Covenant University, Ota, Ogun State, Nigeria

DOI:

https://doi.org/10.3889/oamjms.2021.6794

Keywords:

Antifungal, Moringa oleifera, Fungi, Carica papaya, Phytochemicals

Abstract

BACKGROUND: Plants remain the natural sources of efficacious phytonutrients with beneficial assets to mankind against microbial disorders. Diverse folklores have reported the roles of medicinal plants in the remedies of various disorders in man and animals. Metabolites and pesticides from the plant origin are considered better alternatives due to favorable environmental impact as compared to the synthetic counterparts. Significant economic losses and hindrance of global papaya production are due to fungal diseases. Phytochemicals have made medicinal plants become sources of environmentally friendly alternative antimicrobials.

AIM: This study aimed at assessing the antifungal activity of leaf extracts of Moringa oleifera against phytopathogenic fungi isolated from Carica papaya.

METHODS: n-Hexane, ethyl acetate, ethanol, methanol, and aqueous extracts of M. oleifera leaves were evaluated for their antifungal properties. Agar well-diffusion method was implemented for in vitro screening, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of the extract types against fungal species of Aspergillus, Penicillium, Rhizopus, and Trichoderma.

RESULTS: All the extracts evaluated inhibited fungal growth to some degree, with the aqueous extract exhibiting more inhibitory activities than the organic extracts. There was significant inhibition of fungal development by the tested plant extracts at different concentrations. MIC of the extracts was 15.625 mg/ml while the MFC values ranged between 15.625 and 31.25. In this work, the antifungal activity of M. oleifera was found to be equal or higher than commercially available fungicide, ketoconazole.

CONCLUSION: The results of this study indicate that foliole extracts of M. oleifera have potential for use as biofungicides for plant protection against fungal diseases.

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References

Paray AR, Bhakat M, Mohanty TK, Behare P, Lone SA, Parry UR, et al. Antimicrobial activity of crude aqueous extracts of Moringa oleifera, Azadirachta indica, Carica papaya, Tinospora cordifolia and Curcuma longa against certain bacterial pathogens. J Pharmacogn Phytochem 2018;7:984-94.

Taylor JL, Rabe J, Megaw LJ, Jafer AK, Van Staden J. Towards the scientific validation of traditional medicinal plant. Plant Growth Regular 2001;34:23-37. DOI: https://doi.org/10.1023/A:1013310809275

Nube NS, Afolayon AJ, Okoli AL. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. Afr J Biotechnol 2008;7:1797-806. DOI: https://doi.org/10.5897/AJB07.613

Sule WF, Okonko IO, Omo-Ogun S, Nwanze JC, Ojezele MO, Ojezele OJ, et al. Phytochemical properties and in-vitro antifungal activity of Senna alata Linn. Crude stem bark extract. J Med Plants Res. 2011;5(2):176-83. DOI: https://doi.org/10.3923/rjbsci.2010.275.284

Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci. 2008;4:839-43.

Abdallah ME, Ali HB, Mohamed AE, Mohamed E, Mohamed AA. In vitro antifungal activity of some plant essential oils. Int J Pharmacol 2015;11:56-61.

Shindano J, Kasase C. Moringa (Moringa oleifera): A source of food and nutrition, medicine and industrial products. ACS Symp Ser. 2009;1021:421-67. DOI: https://doi.org/10.1021/bk-2009-1021.ch024

Negri M, Salci TP, Shinobu-Mesquita CS, Capoci IR, Svidzinski TI, Kioshima ES. Early state research on antifungal natural products. Molecules. 2014;19:2925-56. http://doi.org/10.3390/molecules19032925 PMid:24609016 DOI: https://doi.org/10.3390/molecules19032925

Dellavalle PD, Cabrera A, Alem D, Larrañaga P, Ferreira F, Dalla Rizza M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean J Agric Res. 2011;71(2):231-9. DOI: https://doi.org/10.4067/S0718-58392011000200008

Agrios GN. Losses caused by plant diseases. In: Plant Pathology. Oxford, United Kingdom: Elsevier; 2004. p. 29-45.

Joshi S, Kumar P, Pant P, Sati SC. A comparative evaluation of Kumaun Himalayan gymnosperms for their antifungal potential against plant pathogenic fungi. J Phytopharmacol. 2018;7(3):230-41. DOI: https://doi.org/10.31254/phyto.2018.7302

Aisha SB, Kutama AS, Kabir S, Paul AT. Phytochemical screening and antifungal activity of Moringa oleifera on some selected fungi in Dutse, Jigawa State. Global Adv Res J Agric Sci. 2016;5(6):243-8.

Dillard CJ, German JB. Phytochemical; nutraceutical, and human health. A review. J Sci Food Agric. 2000;80:1744-56. DOI: https://doi.org/10.1002/1097-0010(20000915)80:12<1744::AID-JSFA725>3.0.CO;2-W

Pari L, Kumar NA. Hepatoprotective activity of Moringa oleifera on antitubercular drug-induced liver damage in rats. J Med Food. 2002;5(3):171-7. http://doi.org/10.1089/10966200260398206 PMid:12495589 DOI: https://doi.org/10.1089/10966200260398206

Murakami A, Kitazono Y, Jiwajinda S, Koshimizu K, Ohigashi H. Niaziminin, a thiocarbamate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumorpromoter-induced Epstein-Barr virus activation. Planta Med. 1998;64:319-23. http://doi.org/10.1055/s-2006-957442 PMid:9619112 DOI: https://doi.org/10.1055/s-2006-957442

Chuang PH, Lee CW, Chou JY, Murugan M, Shieh BJ, Chen HM. Antifungal activity of crude extracts and essential oil of Moringa oleifera Lam. Bioresour Technol. 2007;98:232-6. http://doi.org/10.1016/j.biortech.2005.11.003 PMid:16406607 DOI: https://doi.org/10.1016/j.biortech.2005.11.003

Vinoth B, Manivasagaperumal R, Rajaravindran M. Phytochemi cal analysis and antibacterial activity of Azadirachta indica Juss. Int J Res Plant Sci. 2012;2(3):50-5.

Sofowora A. Medicinal plants and traditional medicinal in Africa. In: Screening Plants for Bioactive Agents. 2nd ed. Ibadan, Nigeria: Spectrum Books Ltd., Sunshine House; 1993. p. 134-56.

Trease GE, Evans WC. Pharmacognosy: A Physician Guide to Herbal Medicine London. 13th ed. Tindall, London: ELBS/Bailliere; 1989. p. 176-80.

Vollekova A, Kostalova D, Sochorova R. Isoquinoline alkaloids from Mahonia aquiliform stem bark is active against Mallasssezia sp. Foliar Microbiol. 2001;46(2):107-. http://doi.org/11.10.1007/BF02873586 PMid:11501395 DOI: https://doi.org/10.1007/BF02873586

Samie A, Mashau F. Antifungal activities of fifteen Southern African medicinal plants against five Fusarium species. J Med Plants Res. 2013;7(25):1839-48.

Riad SR, Abdallah AM. Antifungal activity of Moringa oleifera oil and seed extract against some plant pathogenic fungi. Middle East J Agric Res. 2014;3(2):242-9.

Al-Huqail AA, Said I, Behiry SI, Mohamed ZM, Salem MZ, Ali HM, et al. Antifungal, antibacterial, and antioxidant activities of Acacia saligna (Labill.) H. L. Wendl. Flower extract: HPLC analysis of phenolic and flavonoid compounds. Molecules. 2019;24(700):1-14. http://doi.org/10.3390/molecules24040700 PMid:30781352 DOI: https://doi.org/10.3390/molecules24040700

Nweke FU. Antifungal activity of petroluem ether extracts of Moringa oleifera leaves and stem bark against some plant pathogenic fungi. J Nat Sci Res. 2015;5(8):1-6.