Association of Vitamin D Receptor Polymorphism (rs2228570, rs1544410, rs7975232, and rs731236) and Macrophage Migration Inhibitory Factor -173 G/C (rs755622) with the Susceptibility of Active Pulmonary Tuberculosis in Makassar, Indonesia

Najdah Hidayah; Irawaty Djaharuddin; Ahyar Ahmad; Rosdiana  Natzir; Ilhamjaya Patellongi; Agussalim Bukhari; Irda Handayani; Andi Tenriola; Subair Subair; Handayani Halik; Muhammad Nasrum Massi

doi:10.3889/oamjms.2021.6859

Authors

Najdah Hidayah Postgraduate Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia https://orcid.org/0000-0002-9811-2080
Irawaty Djaharuddin Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia; Dr. Wahidin Sudirohusodo Makassar Hospital, Makassar, South Sulawesi, Indonesia
Ahyar Ahmad Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia
Rosdiana Natzir Department of Biochemistry, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia
Ilhamjaya Patellongi Department of Physiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia
Agussalim Bukhari Department of Nutrition, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia
Irda Handayani Department of Clinical Pathology, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesiav
Andi Tenriola Postgraduate Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia https://orcid.org/0000-0002-0247-7449
Subair Subair Postgraduate Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Handayani Halik Postgraduate Program, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia; Hasanuddin University Medical Research Center (HUM-RC), Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia
Muhammad Nasrum Massi Hasanuddin University Medical Research Center (HUM-RC), Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia; Department of Clinical Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, South Sulawesi, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6859

Keywords:

Vitamin D receptor, Migration inhibitory factor, Polymorphism, Active tuberculosis, Interferon-gamma release assay

Abstract

BACKGROUND: The study of Vitamin D Receptor (VDR) and Macrophage Migration Inhibitory Factor (MIF) polymorphisms, associated with active pulmonary tuberculosis (ATB) presents varying results.

AIMS: This study aimed to investigate the association between VDR rs2228570, rs1544410, rs7975232, rs731236 and MIF -173 G/C (rs755622) single nucleotide polymorphism (SNP), with susceptibility of developing ATB, and positivity of Interferon Gamma Release Assay (IGRA) results (in household contact).

METHODS AND MATERIAL: This study involved 83 ATB and 73 household contacts in Makassar. We checked IGRA based on ELISA in household contacts by using QuantiFERON TB Gold Plus test, and we found that 61.64% (n = 45) of household contacts had positive IGRA. Polymorphism examination was carried out by Sanger sequencing.

RESULTS: VDR rs2228570 T/T and T/C-T/T were significantly associated with higher risk of active tuberculosis. VDR rs7975232 G/G genotype was associated with an increased risk of developing active TB compared to T/T-T/G. Haplotype analysis of VDR rs2228570, rs1544410, rs7975232, rs731236 and combination with MIF rs755622 demonstrated that TGGTG was observed to have a higher risk of tuberculosis.

CONCLUSIONS:

The combination of VDR and MIF variants may contribute to the susceptibility of active tuberculosis disease.

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