The Effect of Ascorbic Acid on Interleukin-10 and Tumor Necrosis Factor-α Cytokines in Rattus norvegicus with Endometritis

Muhammad Oky Prabudi; M. F. G. Siregar; I. P. A. Nasution; S. Ilyas

doi:10.3889/oamjms.2021.6946

Authors

Muhammad Oky Prabudi Departement of Obstetrics and Gynecology, University of Sumatera Utara, Medan, North Sumatera, Indonesia
M. F. G. Siregar Departement of Obstetrics and Gynecology, University of Sumatera Utara, Medan, North Sumatera, Indonesia
I. P. A. Nasution Departement of Surgery, University of Sumatera Utara, Medan, North Sumatera, Indonesia
S. Ilyas Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan, North Sumatera, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.6946

Keywords:

Ascorbic acid, Cytokine, Endometritis, Interleukin-10, Inflammation, Tumor necrosis factor-α, Mice

Abstract

BACKGROUND: Endometritis is a gynecological disease characterized by inflammation of the endometrial glands and stroma. Inflammatory stimuli or tissue injury induce inflammatory pain through the release of cytokines. Ascorbic acid (AA) is a water-soluble Vitamin that plays a role in inhibiting the production of proinflammatory cytokines and increases the expression of anti-inflammatory cytokines.

AIM: The purpose of this study was to find out the association between administration of AA and inflammatory cytokines in experimental animals Rattus norvegicus with endometritis.

METHODS: The research was conducted using virgin female R. norvegicus laboratory mice weighing 250–300 g and aged 11–12 weeks with an estrus cycle of 5–6 days. Mice with regular oestrous cycles were randomly divided into three groups: group 1 was given 200 L of water orally without Escherichia coli inoculation and represented a negative control. Groups 2 and 3 were inoculated (50 L/rat) E. coli intravaginally, 106 colony-forming unit/mL, Group 2 was not given AA and the other side Group 3 was assigned AA. The interleukin (IL)-10 and tumor necrosis factor (TNF)-α _cytokines examination was carried out by histopathological examination through a biopsy of the endometrial tissue. Hypothesis testing on the data was analyzed by the Kruskal Wallis test using Statistical Package for Social Sciences.

RESULTS: Data from the current study revealed that the highest mean value of IL-10 was found in the negative control group (2.5) and the lowest value in the positive control group (1.3). Regarding TNF-α _the highest mean value (2.8) was found in the treatment group and the lowest mean value (2.1) was found in the treatment group. Using the Kruskal Wallis test, IL-10 and TNF-α _showed insignificant results (p = value 0.304 and 0.145 respectively).

CONCLUSIONS: The administration of AA did not affect the decrease in TNF-α _or the upregulation of IL-10 as anti-inflammatory cytokines.

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