Association of Lipids’ Metabolism with Vitamin D Receptor (rs10735810, rs222857) and Angiotensinogen (rs699) Genes Polymorphism in Essential Hypertensive Patients

Yuliya Repchuk; Larysa Sydorchuk; Larysa Fedoniuk; Zoia Nebesna; Valentyna Vasiuk; Andrii Sydorchukv; Oksana Iftoda

doi:10.3889/oamjms.2021.6975

Authors

Yuliya Repchuk Department of Family Medicine, Bukovinian State Medical University, Chernivtsi, Ukraine https://orcid.org/0000-0002-5156-8814
Larysa Sydorchuk Department of Family Medicine
Larysa Fedoniuk Department of Biology, Ternopil National Medical University, Ternopil, Ukraine
Zoia Nebesna Department of Biology, Ternopil National Medical University, Ternopil, Ukraine
Valentyna Vasiuk Department of Internal Disease, Bukovinian State Medical University, Chernivtsi, Ukraine
Andrii Sydorchukv Department of Family Medicine, Bukovinian State Medical University, Chernivtsi, UkraineDepartment of Family Medicine, Bukovinian State Medical University, Chernivtsi, Ukraine
Oksana Iftoda Department of Family Medicine, Bukovinian State Medical University, Chernivtsi, Ukraine

DOI:

https://doi.org/10.3889/oamjms.2021.6975

Keywords:

Lipids, Cholesterol, Vitamin D receptor gene (VDR rs10735810, rs222857), Angiotensinogen gene (AGT rs699), Arterial hypertension

Abstract

BACKGROUND: Cardiovascular (CV) diseases are the most spread cause of mortality in the world. Essential arterial hypertension (EAH), as a major risk factor for the development of CV diseases, is a multifactorial disease involving environmental and genetic factors together with risk-conferring behaviors.

AIM: The purpose of this study was to analyze lipid metabolism changes in patients with EAH depending on the Vitamin D receptor (VDR rs2228570 (aka rs10735810)) and angiotensinogen (AGT rs699) genes polymorphism.

MATERIALS AND METHODS: The single-stage study involved 100 patients suffering from Stage 2 EAH, 1–3 degrees of blood pressure increase, high and very high CV risks, 21% (21) men, and 79% (79) women. The average age of patients was 59.86 ± 6.22 years old. The control group included 60 practically healthy individuals of an appropriate age and sex distribution. To examine the VDR gene (rs10735810, rs2228570) and AGT gene (rs699) polymorphism, a qualitative real-time polymerase chain reaction was made. The lipid metabolism was studied by determining the blood plasma content of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TGs).

RESULTS: Т allele of AGT gene is associated with reduced HDL-C level in men and increased TGs level in women. The EAH risk increases 4.5 times as much among the ТС-genotype carriers and lowered HDL-C level (odds ratio [OR] = 6.43; p = 0.01). The EAH risk increases as far as the HDL-C level reduction, irrespective of the VDR gene alleles condition 1.83 times (OR = 2.37; OR 95% confidence interval [CI]: 1.02–5.51; p = 0.04) and 1.9 times (OR=2.43; OR 95% CI: 0.99–5.97; p = 0.04). HDL-C reduction and LDL-C elevation in women increase the EAH risk 2.4 times (OR = 3.27; p = 0.01) and 1.24 times (OR = 3.67; p = 0.01), respectively.

CONCLUSIONS: The EAH risk increases with a reduced HDL-C level in the TC genotype carriers of the AGT gene and irrespective of VDR gene polymorphic variants.

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