Antioxidant Activity of Purslane (Portulaca oleracea L.) Leaf Extract on the Levels of Ovarian Oxidative Stress and Reproductive Hormone in Rattus norvegicus Exposed to Cigarette Smoke
DOI:
https://doi.org/10.3889/oamjms.2021.7081Keywords:
17ß-estradiol, Follicle stimulating hormone, Malondialdehyde, Purslane leaf extract, Reproductive health, Tobacco useAbstract
BACKGROUND: Purslane (Portulaca oleracea L.) is a wild plant that grows in Indonesia and is considered a weed, but it has the highest antioxidant activity in the leaves and stems. Cigarette smoke is one of the exogenous free radicals that can enter blood circulation, interfering with all cells and tissues in the body, including reproductive organs.
AIM: The research aims to examine the antioxidant effect of purslane leaf extract on oxidative stress levels (ovarian malondialdehyde [MDA] levels) and reproductive hormone levels (follicle-stimulating hormone [FSH] and 17ß-estradiol) in female white rats exposed to cigarette smoke.
METHODS: This study is an experimental study with randomized post only control group design, using 12-week-old female Wistar strain Rattus norvegicus weighing about 200–250 g divided into four treatment groups (n = 6). Negative control without exposure to cigarette smoke and purslane leaf extract (K), cigarette group with cigarette smoke 1 stick/day administration without purslane leaf extract (P1), purslane group with purslane leaf extract 200 mg/Kg BW without exposure to cigarette smoke (P2), and group the group with cigarette smoke 1 stick/day accompanied by purslane leaf extract 200 mg/Kg BW administration (P3). Ovarian MDA measurement measures MDA levels as an indicator of oxidative stress in the ovaries determined by the spectrophotometry method. The enzyme-linked immunosorbent assay method measured the measurement of FSH and 17ß-estradiol levels in blood taken intracardially through the right ventricle of the heart.
RESULTS: The results showed that purslane leaf extract at a 200 mg/Kg BW dose reduced ovarian MDA levels of female white rats exposed to cigarette smoke significantly with p < 0.05.
CONCLUSION: However, there has been no significant change in FSH and 17ß-estradiol.Downloads
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References
GATS. Global Adults Tobacco Survey Indonesia Report 2011. New Delhi: World Health Organization Regional Office for South-East Asia; 2011.
Agarwal A, Mellado AA, Premkumar, BJ, Sharma A, Sajal G. The effects of oxidative stress on female reproduction: A review. Reprod Biol Endocrinol. 2012;10:49. DOI: https://doi.org/10.1186/1477-7827-10-49
Tandon VM, Gupta BM, Tandon R. Free radicals, reactive oxygen species. J K Practitioner 2005;12(3):143-8.
Halliwell B, Gutteridge JM. Free Radicals is Biology and Medicine. 4th ed. Oxford: Oxford University Press Inc.; 2007.
Uračková Z. Some current insights into oxidative stress. Physiol Res 2010;59(4):459-69. https://doi.org/10.33549/physiolres.931844 PMid:19929132 DOI: https://doi.org/10.33549/physiolres.931844
Giovambattista A, Suescun MO, Nessralla CC, França LR, Spinedi E, Calandra RS. Modulatory effects of leptin on leydig cell function of normal and hyperleptinemic rats. Neuroendocrinology. 2003;78(5):270-9. https://doi.org/10.1159/000074448 DOI: https://doi.org/10.1159/000074448
Oyeyipo IP, Raji Y, Bolarinwa AF. Nicotine alters male reproductive hormones in male albino rats: The role of cessation. J Hum Reprod Sci. 2013;6(1):40-4. https://doi.org/10.4103/0974-1208.112380 PMid:23869150 DOI: https://doi.org/10.4103/0974-1208.112380
Dalimartha S. Atlas of Indonesian Medicinal Plants. Vol. 5. Jakarta: Mother’s Library; 2006.
Zhou YX, Xin HL, Rahman K, Wang SJ, Peng C, Zhang H. Portulaca oleracea L.: A review of phytochemistry and pharmacological effects. BioMed Res Int 2015;2015:925631. https://doi.org/10.1155/2015/925631 PMid:25692148 DOI: https://doi.org/10.1155/2015/925631
Ahangarpour A, Lamoochi Z, Moghaddam HF, Mansouri SM. Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice. Int J Biomed Reprod. 2016;14(3):205-12. PMid:27294220 DOI: https://doi.org/10.29252/ijrm.14.3.205
Zjačić-Rotkvić V, Kavur L, Cigrovski-Berković M. Hormones and aging. Acta Clin Croat. 2010;49(4):549-54. PMid:21830471
Winarsi H. Natural Antioxidants and Free Radicals: Potential and Applications in Health. Yogyakarta: Canisius Publisher; 2007.
Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: Current status and future prospects. J Assoc Physicians India. 2004;52:794-804. PMid:15909857
Hu JP, Zhao XP, Ma XZ, Wang Y, Zheng LJ. Effects of cigarette smoke on aerobic capacity and serum MDA content and SOD activity of animal. Int J Clin Exp Med. 2014;7(11):4461-65. PMid:25550969
Kelly G. The interaction of cigarette smoking and antioxidants. Part I: Diet an carotenoids. Altern Med Rev 2002;7:370-88. PMid:12410622
Sadeu JC, Foster WG. Cigarette smoke condensate exposure delays follicular development and function in a stage dependent manner. Fertil Steril. 2011;95(7):2410-7. https://doi.org/10.1016/j.fertnstert.2011.03.072 DOI: https://doi.org/10.1016/j.fertnstert.2011.03.072
Owj M, Mohseni M, Amirchaghmaghi E, Sadeghi M, Eshrati B, Shabani F. The relationship between passive smoking and ovarian response outcome in ART cycles. Iran J Reprod Med. 1996;5(4):195-8.
Andarwulan N, Batari R, Sandrasari DA, Boilling B, Wijaya H. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem. 2010;121(4):1231-5. DOI: https://doi.org/10.1016/j.foodchem.2010.01.033
Lolo WA, Sudewi S, Edy HJ. Determination of the value of the sun protecting factor (SPF) of purslane herb (Portulaca oleracea L.). J Pharm Sci Clin Res. 2017;2(1):1-5. DOI: https://doi.org/10.20961/jpscr.v2i01.5230
Lim CK, Tiong WN, Loo JL. Antioxidant activity and total phenolic content of different varieties of Portulaca grandiflora. IJPP. Indian J Pract Pediatr. 2014;4(1):2277-928.
Disti S. Chemical Characteristics and Bioactive Components of Extract Powder from Various Parts of Purslane Plant (Portulaca oleracea L.), Diploma Thesis. Indonesia: Andalas University; 2018.
Okafor IA, Nnamah US, Nnaka J. The fertility assessment of normal cyclic Wistar rats following the administration of methanolic extract of Portulaca oleracea: An experimental study. Middle East Fertil Soc J. 2021;26:5. https://doi.org/10.1186/s43043-020-00048-x DOI: https://doi.org/10.1186/s43043-020-00048-x
Hosseini E, Frozanfar M, Payehdar A. The effect of hydroalcoholic extract of purslane on serum concentration of estrogen, progesterone, prolactin and gonadotropins in mature female rats. J Shahrekord Univ Med Sci. 2021;15(5):12-21.
Grestein B, Wood Diana F. Endocrine System. Jakarta: Erlangga; 2010.
Obinna VC, Kagbo HD, Agu GO. Lipophilic and hydrophilic leaf extracts of Portulaca oleracea (Purslane) disrupts female sex hormones in albino rats (Rattus norvegicus). J Tradit Complement Med. 2021;11(2):82-9. DOI: https://doi.org/10.1016/j.jtcme.2019.06.004
Sejian V, Meenambigai TV, Chandirasegaram M, Naqvi SM. Reproductive technology in farm animals: New facets and findings: A review. J Biol Sci. 2010;10(7):68. DOI: https://doi.org/10.3923/jbs.2010.686.700
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Copyright (c) 2020 Astika Gita Ningrum, Endyka Erye Frety, Ivon Diah, Zidni Hasbuna Shabran, Rida Eka Setiani, Erni Rosita Dewi (Author)
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