The Effect of Uncaria gambir Roxb. Extract on Superoxide Dismutase Activity in Proteinuric Wistar Rats Model

Authors

  • Rosmayanti Syafriani Siregar Department of Pediatric
  • Aznan Lelo Department of Pharmacology and Therapeutic, Faculty of Medicine, University Sumatera Utara, Medan, Indonesia
  • Delyuzar Harris Department of Pathology Anatomy, Faculty of Medicine, University Sumatera Utara, Medan, Indonesia
  • Oke Rina Ramayani Department of Pediatric, Faculty of Medicine, University Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0001-6806-2871
  • Muhammad Ichwan Department of Pharmacology and Therapeutic, Faculty of Medicine, University Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0001-8380-9099

DOI:

https://doi.org/10.3889/oamjms.2021.7371

Keywords:

Proteinuric, Catechins, Gambier, Superoxide dismutase

Abstract

Nephrotic syndrome (NS) is a glomerular disease that is most often found in children with proteinuric as clinical manifestation. Gambier extract is a traditional medicine that has antioxidant effect. Its use to treat  proteinuric has never been done. Therefore, we conducted an analytical study on the effect of gambier on proteinuric and superoxide dismutase (SOD) activity in proteinuric Wistar rat’s model. This research is an experimental study with a posttest control group design. The aim is analyzing the effect of giving gambier extract in decreasing urinary protein creatinine ratio and increasing SOD activity. Male Wistar rats were given an injection of puromycin aminonucleoside 1.5mg/100g body weight (BW), subcutaneously for 5 consecutive days for being nephrosis. The experimental animals in this study were divided into four groups: K1, the control group, consist of group of rats that were injected with 0.15ml/100 g BW of aquabidest and not given gambier extract. While K2 group consist of rats induced by Puromycin and given 1 ml of aquabidest. K3 consist of groups of rats induced by Puromycin and then given gambier extract 26mg/200g BW. Group K4 consist of rats induced by Puromycin and then given 80mg/200g BW of gambier extract. The four groups were given treatment for 14 days, after which the rats were placed in a metabolic cage for 24 hours to collect urine samples and then knocked down with ketamine for intra-cardiac blood collection. The results of this study showed differences between the four groups of rats in terms of the mean urine protein/creatinine ratio (p=0.015) and SOD activity (p=0.036). Groups of rats that were given gambier extracts 80mg/200g BW had lower urine protein / creatinine ratio and higher SOD activity. Therapy of gambier 80 mg/200g BW is better in managing proteinuric compared to 26mg/200g.

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Published

2021-11-10

How to Cite

1.
Siregar RS, Lelo A, Harris D, Ramayani OR, Ichwan M. The Effect of Uncaria gambir Roxb. Extract on Superoxide Dismutase Activity in Proteinuric Wistar Rats Model. Open Access Maced J Med Sci [Internet]. 2021 Nov. 10 [cited 2024 Mar. 28];9(B):1505-9. Available from: https://oamjms.eu/index.php/mjms/article/view/7371

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