The Effect of Gembili Starch (Dioscorea esculenta) and Eubacterium rectal Supplementation on Skeletal Muscle Peroxisome Proliferator-Activated Receptor γ Coactivator 1α (Pgc-1α) Expression in Diabetic Mice Models

Tri  Setyawati; Rio  Jati Kusuma; Harry  Freitag Luglio; Neni  Oktiyani; Sunarti Sunarti; Rosmala Nur; Syaiful Hendra

doi:10.3889/oamjms.2021.7415

Authors

Tri Setyawati Department of Biochemistry, Faculty of Medicine, Universitas Tadulako, Indonesia https://orcid.org/0000-0001-5754-2087
Rio Jati Kusuma Department of Health Nutrition, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Harry Freitag Luglio Department of Health Nutrition, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Neni Oktiyani Department of Biochemistry, Poltekkes, Banjarmasin, Indonesia
Sunarti Sunarti Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Rosmala Nur Department of Public Health, Faculty of Public Health, Tadulako University, Palu, Indonesia
Syaiful Hendra Department of Information Technology, Faculty of Engineering, Tadulako University, Palu, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7415

Keywords:

Gembili, Eubacterium rectal, PGC-1α, Glucose tolerance, Diabetes mellitus

Abstract

BACKGROUND: Gembili or Dioscorea esculenta is a local food that is produced by several areas in Indonesia. Few studies have reported its health benefits for diabetes mellitus but a little is understood about its mechanism of action. PGC-1α is a transcriptional coactivator for genes that involved in energy metabolism and increased expression of this gene has previously been associated with improved insulin sensitivity.

AIM: The objective of this study was to investigate the effect of Gembili starch and Gembili starch with butirogenic bacteria Eubacterium rectal on PGC-1α expression in skeletal muscle of diabetic mice.

MATERIALS AND METHODS: Three months old male diabetic Wistar mice were divided into groups based on dietary supplement: Gembili starch only; Gembili starch with E. rectal; and E. rectal only. Positive (diabetic mice) and negative (non-diabetic) control groups were used in this study. After 4 weeks of supplementation, mice were sacrificed and muscle tissue was taken from musculus vastus latissimus. Plasma blood glucose was measured before and after intervention. PGC-1α expression was measured with immunohistochemistry and quantified by dividing cells that produce PGC-1α with total cells.

RESULTS: Plasma blood glucose was reduced after invention in group that received Gembili starch only (p < 0.001); Gembili starch with E. rectal (p < 0.001); and E. rectal only (p < 0.001). The protein expression of PGC-1α in diabetic mice receiving Gembili starch only was significantly higher compared to control (p < 0.05).

CONCLUSION: This study shown that Gembili starch supplementation was able to improve glucose control in diabetic mice and this effect was obtained perhaps through PGC-1α activation. Further study is needed to investigate the effect of Gembili starch supplementation on fat metabolism.

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