Moringa Leaf Powder (Moringa oleifera) Decrease of Inflammation Plasma Cytokine of Pregnant Rats with Diabetes Mellitus

Abstract

BACKGROUND: Preeclampsia is a major obstetric problem worldwide, especially in developing countries that can cause maternal and fetal morbidity and mortality that affect 2–10% of pregnancies worldwide. More than 4 million pregnant women each year develop preeclampsia and cause 15–20% of maternal deaths worldwide. There are several high-risk factors associated with preeclampsia. Diabetes mellitus is one of the risk factors for preeclampsia. Preeclampsia has been shown to have higher levels of Th1 (pro-inflammatory) products and lower levels of Th2 (anti-inflammatory) products compared to normal pregnancy in blood serum. Interleukin (IL) 1, IL-2, IL-8, tumor necrosis factor (TNF)-alpha, and interferon gamma are Th1 cytokines or pro-inflammatory cytokines that can induce inflammatory reactions and are associated with pregnancy complications such as repeated abortion, preterm labor, rupture of membranes. preeclampsia, and stunted fetal growth. In contrast, Th2 cytokines such as transforming growth factor-β (TGF-β), IL-4, IL-5, IL6, and IL-10 are associated with normal pregnancy.

AIM: This study aims to prove the effect of giving Moringa leaf powder (M. oleifera) to decrease blood levels, , so this study will answer the effect of Moringa oleifera leaf powder on blood level of IL-6, IL-10, TNF-α and TGF-β

METHODS: A total of 30 rats were checked for sugar levels on day 4 after being induced by alloxan for 18 days to see if the rats were already in a hyperglycemic state. Then, they were divided into six groups, each group contained five pregnant white rats. Group 1 was the negative group (without being given alloxan), Group 2 was positive (given alloxan 150 mg/day/kg BW), and Groups 3, 4, 5, and 6 were given alloxan 150 mg/day/kg BW and each given a dose of 100 Moringa leaf powder; 200, 400, and 800 mg/day/kg body weight. After alloxan was induced and given a dose of Moringa leaf powder according to each group, blood samples were taken to separate the serum and continued with the ELISA method to calculate the levels of IL-6, IL-10, TNF-α, and TGF-β.

RESULTS: The administration of Moringa leaf powder at a dose of 800 mg/day/kg BW was able to increase the levels of IL-10 in the positive control Group 4 by 7.211 mIU/ml and reduced levels of IL-6 in the 8th dose group by giving Moringa leaf powder as much as 800 mg/day/kg body weight by 2.112 mIU/ml, was able to increase TGF-β levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW, amounted to 1049.066 mIU/ml, and decreased TNF-α levels in the dose Group 4 by giving Moringa leaf powder as much as 800 mg/day/kg BW mIU/ml.

CONCLUSION:The administration of Moringa leaf powder was able to increase the levels of IL-10 in the positive control Group, reduced levels of IL-6, was able to increase TGF-β levels, and decreased TNF-α levels.