Antibacterial Screening of Endophytic Fungus Xylaria sp. derived from Andrographis paniculata (Sambiloto)

Suryelita Suryelita; Riga Riga; Sri Benti Etika; Mariam Ulfah; Muh Ade Artasasta

doi:10.3889/oamjms.2021.7475

Authors

Suryelita Suryelita Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
Riga Riga Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia https://orcid.org/0000-0003-3395-1577
Sri Benti Etika Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang, Padang, Indonesia
Mariam Ulfah Department of Pharmacy, STIKES Muhammadiyah Cirebon, Cirebon, Indonesia
Muh Ade Artasasta Department of Biotechnology, Faculty of Mathematics and Natural Sciences, University Negeri Malang, Malang, Indonesia https://orcid.org/0000-0003-4904-201X

DOI:

https://doi.org/10.3889/oamjms.2021.7475

Keywords:

Andrographis paniculata, Antibacterial activity, Endophytic fungus, Xylaria sp.

Abstract

AIM: The purpose of this research is to evaluate the antibacterial activity of endophytic fungi derived from the flowers of Andrographis paniculata (Sambiloto).

METHODS: The endophytic fungi were obtained following the dilution method with potato dextrose agar as media. Four isolates of fungi have been obtained and then fermented with rice media for 3 weeks. The fermented fungi were extracted with ethyl acetate (EtOAc) and evaporated to yield the EtOAc extract. All EtOAc extracts were evaluated for their antibacterial activity using agar diffusion method.

RESULTS: The results indicated that the EtOAc extract from fungus RG-2 was the potential source of antibacterial compounds. Molecular identification showing fungus RG-2 was Xylaria sp.

CONCLUSION: Further investigation of the antibacterial compounds produced by fungus Xylaria sp. derived from the flowers of A. paniculata will be performed in the future. To the best of our knowledge, endophytic fungal Xylaria sp. is firstly isolated from A. paniculata.

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