Effect of Enhydra fluctuans on Kidney Function in Alloxan-induced Diabetic Rats

Rina Delfita; Dahelmi Dahelmi; Djong Tjong; Suhatri Suhatri

doi:10.3889/oamjms.2021.7531

Authors

Rina Delfita Department of Biology Education, Faculty of Tarbiyah and Teacher Training, IAIN Batusangkar, West Sumatera, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, West Sumatera, Indonesia
Dahelmi Dahelmi Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, West Sumatera, Indonesia
Djong Tjong Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, West Sumatera, Indonesia
Suhatri Suhatri Faculty of Pharmacy, Andalas University, West Sumatera, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7531

Keywords:

Alloxan, Creatinine, Diabetes mellitus, Enhydra fluctuans, Kidney

Abstract

AIM: The aim of this study was to explore the effect of n-hexane fraction of Enhydra fluctuans aerial on kidney function in alloxan induced diabetic rats.

METHODS: Five groups of diabetic Wistar rats were studied: Group 1 was given 0.5% Na-CMC (G0), group 2 was given glibenclamide 0.45 mg/kg (G1), groups 3, 4, and 5 were given a dose of n-hexane fraction 57.03, 114.06, and 171.09 mg/kg respectively. The experiment was completed in 21 days. Blood glucose was estimated on day 0 and day 21 of treatment. Histology of kidney, creatinine, and blood urea nitrogen (BUN) was examined. ANOVA was used to evaluate quantitative data, which was then followed by Duncan's new multiple range test (p < 0.05).

RESULTS: Our results demonstrate that n-hexane fraction dosages of 57.03 mg/kg and 114,06 mg/kg significantly improved blood glucose profile, BUN, and creatinine in diabetic rats. Moreover, the dosage of 57.03 mg/kg is effective to counteract necrosis and fibrosis of kidney cells.

CONCLUSION: Our findings revealed that the administration of the n-hexane fraction of E. fluctuans aerial improved the kidney function of diabetic rats, especially at the dosage of 57.03 mg/kg. Therefore, E. fluctuans can be relied upon to be a drug to prevent the development of diabetes mellitus and diabetic nephropathy.

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