Antioxidant and Anti-inflammatory Activity of γ-Oryzanol Compared to Rice Bran Oil to Repair Ovarian Histological Structure from One Push Transfluthrin Exposure Effect

Lilis Lisnawati; Sri Poeranto; Agustina Tri Endharti; Moch. Istiadjid Edi Santoso

doi:10.3889/oamjms.2022.7543

Authors

Lilis Lisnawati Doctoral Program of Medical Science, Faculty of Medicine, University Brawijaya, Malang, East Java, Indonesia; Midwifery Study Program, College of Health Science General Respati, Tasikmalaya, West Java, Indonesia https://orcid.org/0000-0003-3791-073X
Sri Poeranto Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
Agustina Tri Endharti Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia; Master Program of Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
Moch. Istiadjid Edi Santoso Department of Health Research Ethics Commission, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.7543

Keywords:

Follicle, Ovaries, γ-Oryzanol, Rice bran oil, Transfluthrin

Abstract

BACKGROUND: Fertility is affected by both the reproductive organs and external factors (genetics, hormones, radiation exposure, use of insecticides, and nutrition). The histological structure of the ovaries is an indicator of reproductive function. Insect repellent use with pyrethroid active ingredients and its impact on health has become a discussion in the medical sector for years. Disruption of the reproductive system homeostasis may cause several issues, from disruption of ovarian function to infertility. γ-Oryzanol has higher antioxidants than vitamin E. It is found mostly in rice bran oil (RBO). Researchers have investigated the effectiveness of RBO and γ-Oryzanol, but the number of studies focusing on the reproductive system is very limited. Results of in silico showed anti-inflammatory potential, and nitric oxide γ-Oryzanol is stronger than the antioxidant activity. It also showed γ-Oryzanol bond with Foxo3a and Growth Differentiating Factor 9 (GDF9), indicating the γ-Oryzanol potential for reproductive health (women). Studies also reported that γ-Oryzanol administration caused anti-inflammatory and antioxidant activity compared to RBO in improving ovarian physiological function using tumor necrosis factor α (TNF-α) levels, Foxo3a expression, and GDF9 expression exposed to one push transfluthrin as the parameter.

AIM: We aimed to investigate antioxidant and anti-inflammatory activity of γ-Oryzanol compared to rice bran oil to repair ovarian histological structure from one push transfluthrin exposure effect.

METHODS: Experimental research, post-test only control group design approach, with a completely randomized design, consisted of 6 (six) groups of Wistar strain female rats. They were exposed to one push with the active ingredient of transfluthrin 21.3%, by inhalation for 6 hours, RBO 0.3 mg/g/ body weight (BW)/day, γ-Oryzanol 3.75 ml/g/BW/day. Statistical analysis was done with the Mann Whitney’s posthoc Kruskal Wallis test with IBM SPSS version 25 software.

RESULTS: γ-Oryzanol had more potent anti-inflammatory and antioxidant activity than RBO in improving the ovarian histology structure (including maintaining ovarian weight, increasing follicular growth, and suppressing follicular abnormalities) through decreased TNF-α levels and decreased Foxo3a expression, and increased GDF9 expression.

CONCLUSION: The administration of γ-Oryzanol improves the ovarian histological structure from free radicals effects, namely exposure to one push of transfluthrin.

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