Relationship of Anti-interleukin-6 Receptor Antibody to Interleukin-6 Level and Inducible Nitrite Oxide Levels in Peripheral Nerve Injury in Chronic Constriction Injury-Induced Rats: A Case-Control Study in Indonesia
Keywords:Neuropathic pain, Peripheral nerve injury, Interleukin-6, Inducible nitric oxide
BACKGROUND: Interleukin-6 (IL-6) and inducible Nitric oxide Synthase (iNOS) have an effect on neuropathic pain in the inflammatory process in peripheral nerve injuries.
AIM: This study aims to examine the effect of anti-IL-6 receptor antibody on IL-6 and iNOS levels as a consideration for the treatment of neuropathic pain in a rat model of peripheral nerve injury.
METHODS: Twenty-eight young adult male Wistar rats were treated for peripheral nerve injury and then divided into two groups. Fourteen treatment groups (Group P) were given anti-IL-6 receptor antibody by injection at a dose of 100 g/day by injection into the saphenous vein in the rat’s leg for 3 days. In both groups, the serum IL-6 and iNOS levels were assessed on the 3rd day after administration of anti-IL-6 receptor antibody in group P, using the sandwich ELISA method.
RESULTS: The results showed that the administration of anti-IL-6 receptor antibody did not have a significant effect on reducing IL-6 and iNOS levels in group P (p > 0.05). Administration of anti-IL-6 receptor antibody had more effect on IL-6 levels on iNOS levels, where a decrease in IL-6 levels caused a decrease in iNOS levels in group P (p = 0.004 and r = 0.693).CONCLUSIONS: We conclude that the present administration of anti-IL-6 receptor antibody cannot be considered as a treatment for neuropathic pain in peripheral nerve injuries, but can be used to influence IL-6 levels on iNOS levels.
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Nicholson B. Differential diagnosis: Nociceptive and neuropathic pain. Am J Manag Care. 2006;12 Suppl 9:S256-62. PMid:16774457
Borda AP, Sonnek FC, Fonteyne V, Papaioannou EG. Guidelines on Pain Management and Palliative Care. Arnhem, Netherlands: European Association of Urology; 2013. p. 70.
Akkurt HE, Gümüs H, Göksu H, Odaba OF, Yilmaz H. Gabapentin treatment for neuropathic pain in a child with sciatic nerve injury. Case Rep Med. 2015;2015:873157. DOI: https://doi.org/10.1155/2015/873157
McMahon SB, Malcangio M. Current challenges in glia-pain biology. Neuron. 2009;64(1):46-54. https://doi.org/10.1016/j.neuron.2009.09.033 PMid:19840548 DOI: https://doi.org/10.1016/j.neuron.2009.09.033
McCarberg BH, Billington R. Consequences of neuropathic pain: Quality-of-life issues and associated costs. Am J Manag Care. 2006;12 Suppl 9:S263-8. PMid:16774458
Childers WE Jr., Baudy RB. N-methyl-D-aspartate antagonists and neuropathic pain: The search for relief. J Med Chem. 2007;50(11):2557-62. https://doi.org/10.10a/jm060728b PMid:17489572 DOI: https://doi.org/10.1021/jm060728b
Inoue K, Tsuda M. Microglia and neuropathic pain. Glia. 2009;57(14):1469-79. https://doi.org/10.1002/glia.20871 PMid:19306358 DOI: https://doi.org/10.1002/glia.20871
Milligan ED, Watkins LR. Pathological and protective roles of glia in chronic pain. Nat Rev Neurosci. 2009;10(1):23-36. https://doi.org/10.1038/nrn2533 PMid:19096368 DOI: https://doi.org/10.1038/nrn2533
Zhuo M. A synaptic model for pain: Long-term potentiation in the anterior cingulate cortex. Mol Cells. 2007;23(3):259-71. PMid:17646700
Kandel ER. The molecular biology of memory storage: A dialogue between genes and synapses. Science. 2001;294(5544):1030-8. https://doi.org/10.1126/science.1067020 PMid:11691980 DOI: https://doi.org/10.1126/science.1067020
Vallance P, Leiper J. Blocking no synthesis: How, where and why? Nat Rev Drug Discov. 2002;1(12):939-50. https://doi.org/10.1038/nrd960 PMid:12461516 DOI: https://doi.org/10.1038/nrd960
Zhang W, Liu LY, Xu TL. Reduced potassium-chloride co-transporter expression in spinal cord dorsal horn neurons contributes to inflammatory pain hypersensitivity in rats. Neuroscience. 2008;152(2):502-10. https://doi.org/10.1016/j.neuroscience.2007.12.037 PMid:18262726 DOI: https://doi.org/10.1016/j.neuroscience.2007.12.037
Li P, Wilding TJ, Kim SJ, Calejesan AA, Huettner JE, Zhuo M. Kainate-receptor-mediated sensory synaptic transmission in mammalian spinal cord. Nature. 1999;397(6715):161-4. https://doi.org/10.1038/16469 PMid:9923678 DOI: https://doi.org/10.1038/16469
Wu LJ, Zhuo M. Targeting the NMDA receptor subunit NR2B for the treatment of neuropathic pain. Neurotherapeutics. 2009;6(4):693-702. https://doi.org/10.1016/j.nurt.2009.07.008 PMid:19789073 DOI: https://doi.org/10.1016/j.nurt.2009.07.008
Ji RR, Kohno T, Moore KA, Woolf CJ. Central sensitization and LTP: Do pain and memory share similar mechanisms? Trends Neurosci. 2003;26(12):696-705. https://doi.org/10.1016/j.tins.2003.09.017 PMid:14624855 DOI: https://doi.org/10.1016/j.tins.2003.09.017
Ikeda H, Heinke B, Ruscheweyh R, Sandkuhler J. Synaptic plasticity in spinal lamina I projection neurons that mediate hyperalgesia. Science. 2003;299(5610):1237-40. https://doi.org/10.1126/science.1080659 PMid:12595694 DOI: https://doi.org/10.1126/science.1080659
Sandkuhler J. Understanding LTP in pain pathways. Mol Pain. 2007;3:9. https://doi.org/10.1186/1744-8069-3-9. PMid:17407590 DOI: https://doi.org/10.1186/1744-8069-3-9
Murakami A, Kanchiku T. Anti-interleukin-6 receptor antibody reduces neuropathic pain following spinal cord injury in mice. Exp Ther Med. 2013;6(5):1194-8. https://doi.org/10.3892/etm.2013.1296 PMid:24223643 DOI: https://doi.org/10.3892/etm.2013.1296
Kolios G, Brown Z, Robson RL, Robertson DA, Westwick J. Inducible nitric oxide synthase activity and expression in a human colonic epithelial cell line, HT-29. Br J Pharmacol. 1995;116(7):2866-72. https://doi.org/10.1111/j.1476-5381.1995.tb15938.x PMid:8680718 DOI: https://doi.org/10.1111/j.1476-5381.1995.tb15938.x
Amin B, Hosseinzadeh H. Evaluation of aqueous and ethanolic extracts of saffron, Crocus sativus L., and its constituents, safranal and crocin in allodynia and hyperalgesia induced by chronic constriction injury model of neuropathic pain in rats. Fitoterapia. 2012;83(5):888-95. https://doi.org/10.1016/j.fitote.2012.03.022 PMid:22484092 DOI: https://doi.org/10.1016/j.fitote.2012.03.022
Bhardwaj HC, Muthuraman A, Hari KS, Navis S. Antioxidative and anti-inflammatory potentials of ambroxol in ameliorating vincristine induced peripheral neuropathic pain in rats. J Neuroinfect Dis. 2016;7:202.
Fregnan F, Muratori L, Simões AR, Giacobini-Robecchi MG, Raimondo S. Role of inflammatory cytokines in peripheral nerve injury. Neural Regen Res. 2012;7(29):2259-66. https://doi.org/10.3969/j.issn.1673-5374.2012.29.003 PMid:25538747
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