The Potential of Centella asiatica Herb and Cyperus rotundus L. Rhizomes Extract as a Chemoprevention Agent for Lung Cancer


  • Indrayanti Indrayanti Department of Pathology Anatomy, Faculty of Medicine and Health Science, Universitas Muhammadiyah, Yogyakarta, Indonesia
  • Titiek Hidayati Department of Public Health and Family Medicine, Faculty of Medicine and Health Science, Universitas Muhammadiyah, Yogyakarta, Indonesia image/svg+xml
  • Arif B. Setyanto Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia image/svg+xml



Lung cancer, Chemopreventive, Centella asiatica herb, Cyperus rotundus rhizome


BACKGROUND: The number of smokers is increasing in Indonesia. Cigarette smoke can cause many diseases, such as lung cancer. Centella asiatica (CAS) and Cyperus rotundus L. rhizomes (CRR) exhibit anti-cancer effects.

AIM: The study examined the cytotoxic effects of CRR and CAS extracts in lung cancer cells.

METHODOLOGY: This research used the maceration method to extract the CAS and CRR powder and methanol solvent. Extraction was tested at 600μl, 400μl, 200 μl, 100 μl, and 50 μl to determine the effect of compound cytotoxicity causing 50% cell death or IC50. Cytotoxicity tests used the MTT method to obtain purple formation crystals and used an ELISA reader to obtain absorbance values.

RESULTS: CRR and CAS extract shows a low cytotoxicity effect. IC50 of CRR and CAS methanol extracts was 235 μg/ml and 279 μg/ml, respectively.

CONCLUSION: CRR and CAS extracts were proven to show chemopreventive activities against lung cancer cells.


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IAKMI T. Atlas Tembakau Indonesia Tahun; 2020. p. 33. Available from: [Last accessed on 2021 Aug 20].

Yang GH, Li Q, Wang C, Hsia J, Yang Y, Xiao L, et al. Findings from 2010 global adult tobacco survey: Implementation of MPOWER policy in China. Biomed Environ Sci. 2010;23(6):422-9. PMid:21315239 DOI:

Hardesty JJ, Kaplan B, Martini S, Megatsari H, Kennedy RD, Cohen JE. Smoking among female daily smokers in Surabaya, Indonesia. Public Health. 2019;172:40-2. PMid:31158567 DOI:

Hidayati T, Akrom A, Apriani L. The effect of physical activity on lymphocyte count in smokers who consume black cumin seed (Nigella sativa L.) oil. Int J Public Health Sci. 2020;9(1):8-14. DOI:

Hidayati T, Pramono A, Jenie IM, Soesatyo MH. Evaluation of black cumin seeds hexane extract as reactive oxygen intermediates (ROI) and phagocytic activity modulator in DMBA inducedrats. Biomed Res. 2017;28(4):1755-60.

Kementerian Kesehatan RI, Panduan Penatalaksanaan Kanker Paru. Jakarta: Pusat Data dan Informasi Kementerian Kesehatan RI; 2017.

Hidayati T, Akrom A, Indrayanti, Sagiran. Chemopreventive effect of black cumin seed oil (BCSO) by increasing p53 expression in dimethylbenzanthracene (DMBA)-induced Sprague Dawley rats. Res J Chem Environ. 2019;23(8):24-32. DOI:

Menteri Kesehatan Republik Indonesia. Keputusan Menteri Kesehatan Republik Indonesia Nomor 796/Menkes/SK/VII/2010 tentang Pedoman Teknis Pengendalian Kanker Payudara dan Kanker Leher Rahim; 2010. p. 1-69. Available from: [Last accessed on 2021 Aug 20].

Crawford J, Dale DC, Lyman GH. Chemotherapy-induced neutropenia: Risks, consequences, and new directions for its management. Cancer. 2004;100(2):228-37. PMid:14716755 DOI:

Fajar DR, Akrom, Darmawan E. The Influence of Black Cumin Seed Oil Therapy with Dosage of 1.5 mL/day and 3 mL/day to Interleukin-21 (IL-21) Expression of the Patients with Metabolic Syndrome Risk. Vol. 259. IOP Conference Series: Materials Science and Engineering, 2017. DOI:

Akrom A, Kes M, Prasetyawan N. Chewable tablets of ethanol extract of gotu kola herb (Centella asiatica (L.), Urban) reduced creatinine levels of male white rats (Rattus norvegicus L.) wistar strain fed a high fat diet. Pharmaciana. 2016;6(2):123-30. DOI:

Nie X, Zhang H, Shi X, Zhao J, Chen Y, Wu F, et al. Asiaticoside nitric oxide gel accelerates diabetic cutaneous ulcers healing by activating Wnt/β-catenin signaling pathway. Int Immunopharmacol. 2019;79:106109. PMid:31865242 DOI:

Hidayati T, Akrom, Indrayanti, Sagiran. Evaluation of the black cumin seed oil role (BCSO) on a decline in eNOS expression and plasma NO levels: Initial studies kemopreventive BCSO for lung cancer. Int J Biosci Biochem Bioinforma. 2017;7(3):162-8. DOI:

Akrom A, Mustofa M. Black cumin seed oil increases phagocytic activity and secretion of IL-12 by macrophages. Biomed Res. 2017;28(12):5241-6.

Gambaran Penggunaan Ramuan Herbal Antihipertensi Di Ru-Mah Riset Jamu Hortus Medicus Tawangmangu. Seminar Nasional Tumbuhan Obat Indonesia ke-55, 17-18 Oktober 2018. Jawa Tengah, Indonesia: Magelang Universitas Tidar dan Kelompok Kerja Nasional Tumbuhan Obat IndonesiaAt: Magelang; 2018.

Agustiarini V, Darmawan E. The effects of dosage variation in black cumin seed oil (Nigella sativa L.) use on HbA1c levels and interleukin-17A expression in patients at risks of metabolic syndrome. Pharmaciana 2019;9(2):261-70. DOI:

Kang YJ, Park KK, Chung WY, Hwang JK, Lee SK. Xanthorrhizol, a natural sesquiterpenoid, induces apoptosis and growth arrest in HCT116 human colon cancer cells. J Pharmacol Sci. 2009;111(3):276-84. PMid:19926935 DOI:

Wu T, Geng J, Guo W, Gao J, Zhu X. Asiatic acid inhibits lung cancer cell growth in vitro and in vivo by destroying mitochondria. Acta Pharm Sin B. 2017;7(1):65-72. PMid:28119810 DOI:

Akrom, Hastanto FA, Nurani LH. Hepatoprotective effect of chewable tablet of Centella asiatica (L.) Urb extractin Wistar rats induced by high fat diets. Indones J Pharmacol Ther. 2021;2(1):1-6. DOI:

Wang X, Cai X, Wang W, Jin Y, Chen M, Huang X, et al. Effect of asiaticoside on endothelial cells inhypoxia-induced pulmonary hypertension. Mol Med Rep. 2018;17(2):2893-900. PMid:29257311 DOI:

de Andrade Luz L, Rossato FA, Costa RA, Napoleão TH, Paiva PM, Coelho LC. Cytotoxicity of the coagulant Moringa oleifera lectin (cMoL) to B16-F10 melanoma cells. Toxicol Vitr. 2017;44:94-9. DOI:

Anas Y, Imron A, Ningtyas I. Ekstrak daun kelor (Moringa oleifera Lam.) sebagai peluruh kalsium batu ginjal secara in vitro.

Baldisserotto A, Buso P, Radice M, Dissette V, Lampronti I, Gambari R, et al. Moringa oleifera leaf extracts as multifunctional ingredients for “natural and organic” sunscreens and photoprotective preparations. Molecules, 2018;23(3):664. PMid:29543741 DOI:

Ha JH, Jayaraman M, Radhakrishnan R, Gomathinayagam R, Yan M, Song YS, et al. Differential effects of thymoquinone on lysophosphatidic acid-induced oncogenic pathways in ovarian cancer cells. J Tradit Complement Med. 2020;10(3):207-16. DOI:

de Siqueira Leite KC, Garcia LF, Lobón GS, Thomaz DV, Moreno EK, de Carvalho MF, et al. Antioxidant activity evaluation of dried herbal extracts: An electroanalytical approach. Rev Bras Farmacogn. 2018;28(3):325-32. DOI:

Lv J, Sharma A, Zhang T, Wu Y, Ding X. Pharmacological review on asiatic acid and its derivatives: A potential compound. SLAS Technol. 2018;23(2):111-27. PMid:29361877 DOI:

Kakarla L, Katragadda SB, Tiwari AK, Kotamraju KS, Madhusudana K, Kumar DA, et al. Free radical scavenging, α-glucosidase inhibitory and anti-inflammatory constituents from Indian sedges, Cyperus scariosus R.Br and Cyperus rotundus L. Pharmacogn Mag. 2016;12(47):S488-96. PMid:27761080 DOI:

Kamala A, Middha SK, Karigar CS. Plants in traditional medicine with special reference to Cyperus rotundus L.: A review. 3 Biotech. 2018;8(7):309. PMid:30002998 DOI:

Ghannadi A, RAbbani M, Ghaemmaghami L, Malekian N. Phytochemical screening and essential oil analysis of one of the Persian sedges, Cyperus rotundus L. Int J Pharm Sci 2012;3:424-7.

Soumaya KJ, Zied G, Nouha N, Mounira K, Kamel G, Genviève FD, et al. Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus. Asian Pac J Trop Med. 2014;7(2):105-12. PMid:24461522 DOI:




How to Cite

Indrayanti I, Hidayati T, Setyanto AB. The Potential of Centella asiatica Herb and Cyperus rotundus L. Rhizomes Extract as a Chemoprevention Agent for Lung Cancer. Open Access Maced J Med Sci [Internet]. 2022 Mar. 13 [cited 2023 Mar. 28];10(A):1132-8. Available from: