Hepatoprotective Activity of Pirdot Leaves (Saurauia vulcani Korth) Ethanol Extract in Laboratory Rats (Rattus norvegicus) and Characterization of Bioactive Compounds Using a Molecular Docking Approach
DOI:
https://doi.org/10.3889/oamjms.2021.7624Keywords:
Pirdot leaves, Serum glutamic pyruvic transaminase, Serum glutamic oxaloacetic transaminase, Cyclooxygenase-2 and tumour necrosis factor alphaAbstract
AIM: The hepatoprotective activities of bioactive compounds Pirdot were investigated in vivo and in silico.
METHODS: In this study, the completely randomized design non-factorial was experimentally to assess the value of SGPT and SGOT and twenty four adult male rats were divided into four groups : group G0, control group; group G1, a treated group received 0.1 ml sheep red blood cell; group G2, a treated group received 500 mg ethanol extract Pirdot; group G3, a group treated received 500 mg ethanol extract Pirdot and 0,1 ml sheep red blood cell. On thirty one days of treatment, the blood of all rats group were taken to value SGPT and SGOT using DiaLab kit. Furthermore, the molecular docking study was done to analyse molecular interaction that COX-2 and TNF-α were the primary target protein of bioactive compounds of Pirdot associated with hepatoprotective activities. In addition, it tends to be the target of non-steroidal anti-inflammatory drugs such as Ibuprofen.
RESULTS: The results show SGOT and SGPT value significantly [p<0.05] decreased on Group G2 and G3. Moreover, the bioactive compounds of Pirdot, such as Pomolic acid and Ursolic acid tend to be the potential compound on liver protection. Moreover, Pomolic acid has a good binding affinity -14.6 kcal mol-1 with COX-2 Protein and the binding affinity of cis-3-O-p-hydroxycinnamoyl Ursolic acid was -15.1 kcal mol-1 associated with TNF-α Protein.
CONLUSION: Pirdot Leaves (Saurauia vulcani Korth.) Ethanol Extract showed Hepatoprotective activity in rats (Rattus norvegicus). Molecular docking approach showed that pomolic acid has a good binding affinity with COX-2 Protein and TNF-α Protein.
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Ilyas U, Katare DP, Aeri V, Naseef PP. A Review on hepatoprotective and immunomodulatory herbal plants. Pharmacogn Rev. 2016;10(19):66-70. https://doi.org/10.4103/0973-7847.176544 PMid:27041876 DOI: https://doi.org/10.4103/0973-7847.176544
Ju C, Phillips E, Holt MP, Gao YR, Lammert C.The adaptive immune system and liver toxicity. In: Third E, editor. McQueen CABT-CT. Oxford: Elsevier; 2018. p. 346-67. https://doi.org/10.1016/b978-0-12-801238-3.95663-0 DOI: https://doi.org/10.1016/B978-0-12-801238-3.95663-0
Villanueva-Toledo JR, Chale-Dzul J, Castillo-Bautista C, Olivera-Castillo L, Rangel-Méndez JA, Graniel-Sabido MJ, et al. Hepatoprotective effect of an ethanol extract of Tradescantia pallida against CCl4-induced liver damage in rats. S Afr J Bot. 2020;135:444-50. https://doi.org/10.1016/j.sajb.2020.09.031 DOI: https://doi.org/10.1016/j.sajb.2020.09.031
Tarasenko TN, McGuire PJ. The liver is a metabolic and immunologic organ: A reconsideration of metabolic decompensation due to infection in inborn errors of metabolism (IEM). Mol Genet Metab. 2017;121(4):283-8. https://doi.org/10.1016/j.ymgme.2017.06.010 PMid:28666653 DOI: https://doi.org/10.1016/j.ymgme.2017.06.010
Horst AK, Neumann K, Diehl L, Tiegs G. Modulation of liver tolerance by conventional and nonconventional antigen-presenting cells and regulatory immune cells. Cell Mol Immunol. 2016;13(3):277-92. https://doi.org/10.1038/cmi.2015.112 PMid:27041638 DOI: https://doi.org/10.1038/cmi.2015.112
Shah VV, Shah VK, Sheth NR, Patel MM. Hepatoprotective and Anti-inflammatory activities of hydroalcoholic extract of bark of Erythrina indica. Res J Pharmacol Pharmacodyn 2017;9(4):189-94. https://doi.org/10.5958/2321-5836.2017.00033.7 DOI: https://doi.org/10.5958/2321-5836.2017.00033.7
Babu G, Shalima NK, Divya TA. Evaluation of hepatoprotective activity of rhizomes of Curculigo orchioides Gaertn. Res J Pharm Technol. 2013;6(10):1127-30.
Sitorus P. Characterization simplisia and ethanolic extract of pirdot (Saurauia vulcani, Korth) leaves and study of antidiabetic effect in alloxan induced diabetic mice. Int J Chemtech Res. 2015;8:789-94.
Situmeang BR, Suparman AR, Kadarusman M, Parumbak AS, Herlina T. Isolasi senyawa triterpenoid dari ekstrak etil asetat pirdot (Saurauia vulcani. Kurth). J Kim Valensi. 2018;4(2):93-7. DOI: https://doi.org/10.15408/jkv.v4i2.7272
Lovena TN, Harahap U, Sitorus P, Satria D. Antioxidant activity and α-glucosidase inhibition effect of water extract Saurauia vulcani Korth leaves. J Innov Pharm Biol Sci 2018;5(3):47-51.
Sinaga E, Ilyas S, Hutahaean S, Sitorus P. Immunostimulatory activity from pirdot leaves ethanolic extract (Saurauia vulcani Korth.) in Rats (Rattus norvegicus). IOP Conf Ser Earth Environ Sci. 2019;305(1):012082. https://doi.org/10.1088/1755-1315/305/1/012082 DOI: https://doi.org/10.1088/1755-1315/305/1/012082
Kumar Gupta R, Swain SR, Sahoo J, Gupta A, Chaudhary S. Hepatoprotective potential of Trichosanthes dioica Roxb in hepatotoxicity induced by simvastatin and its consequences on biochemical and haematological indices. Pharmacogn J 2018;10(4):720-4. https://doi.org/10.5530/pj.2018.4.120 DOI: https://doi.org/10.5530/pj.2018.4.120
Araya EM, Adamu BA, Periasamy G, Sintayehu B, Gebrelibanos Hiben M. In vivo hepatoprotective and In vitro radical scavenging activities of Cucumis ficifolius A. rich root extract. J Ethnopharmacol. 2019;242:112031. https://doi.org/10.1016/j.jep.2019.112031 PMid:31220599 DOI: https://doi.org/10.1016/j.jep.2019.112031
Vasanthkumar T, Hanumanthappa M, Hanumanthappa SK. Hepatoprotective effect of curcumin and capsaicin against lipopolysaccharide induced liver damage in mice. Pharmacogn J. 2017;9(6):947-51. https://doi.org/10.5530/pj.2017.6.148 DOI: https://doi.org/10.5530/pj.2017.6.148
Rongngern P, Chularojanamontri L, Wongpraparut C, Silpa-Archa N, Chotiyaputta W, Pongpaibul A, et al. Diagnostic performance of transient elastography for detection of methotrexate-induced liver injury using Roenigk classification in Asian patients with psoriasis: A retrospective study. Arch Dermatol. 2017;309(5):403-8. https://doi.org/10.1007/s00403-017-1733-4 PMid:28303329 DOI: https://doi.org/10.1007/s00403-017-1733-4
Pasaribu G, Budianto E, Herry C, Saepudin E. A review on genus saurauia: Chemical compounds and their biological activity. Pharmacogn J. 2020;12:657-66. https://doi.org/10.5530/pj.2020.12.97 DOI: https://doi.org/10.5530/pj.2020.12.97
Orlando BJ, Lucido MJ, Malkowski MG. The structure of ibuprofen bound to cyclooxygenase-2. J Struct Biol. 2015;189(1):62-6. https://doi.org/10.1016/j.jsb.2014.11.005 PMid:25463020 DOI: https://doi.org/10.1016/j.jsb.2014.11.005
Khanal P, Patil BM. In vitro and in silico anti-oxidant, cytotoxicity and biological activities of Ficus benghalensis and Duranta repens. Chin Herb Med. 2020;12(4):406-13. http://doi.org/10.1016/j.chmed.2020.02.004 DOI: https://doi.org/10.1016/j.chmed.2020.02.004
Kleiner DE. Histopathological challenges in suspected drug-induced liver injury. Liver Int. 2018;38(2):198-209. https://doi.org/10.1111/liv.13584 PMid:28865179 DOI: https://doi.org/10.1111/liv.13584
Roth NC, Qin J. Histopathology of alcohol-related liver diseases. Clin Liver Dis. 2019;23(1):11-23. https://doi.org/10.1016/j.cld.2018.09.001 PMid:30454825 DOI: https://doi.org/10.1016/j.cld.2018.09.001
Ma X, Zhang M, Fang G, Cheng C, Wang M, Han Y, et al. Ursolic acid reduces hepatocellular apoptosis and alleviates alcohol-induced liver injury via irreversible inhibition of CASP3 in vivo. Acta Pharmacol Sin. 2020;42(7):1101-10. http://doi.org/10.1038/s41401-020-00534-y PMid:33028983 DOI: https://doi.org/10.1038/s41401-020-00534-y
Dzoyem JP, Nganteng DN, Melong R, Wafo P, Ngadjui B, Allémann E, et al. Bioguided identification of pentacyclic triterpenoids as anti-inflammatory bioactive constituents of Ocimum gratissimum extract. J Ethnopharmacol. 2021;268:113637. https://doi.org/10.1016/j.jep.2020.113637 DOI: https://doi.org/10.1016/j.jep.2020.113637
Suryaningsih NM, Dewi IA, Suksmawati NK, Putri NP, Febrianti NM, Warditiani NK. Pengaruh kadar SGOT SGPT dan morfologi hepar tikus putih betina wistar pada pemberian isolat andrografolid. J Farm Udayana. 2017;6(1):34-8. DOI: https://doi.org/10.24843/JFU.2017.v06.i01.p06
Cai J, Zhang XJ, Li H. Role of innate immune signaling in non-alcoholic fatty liver disease. Trends Endocrinol Metab. 2018;29(10):712-22. https://doi.org/10.1016/j.tem.2018.08.003 DOI: https://doi.org/10.1016/j.tem.2018.08.003
Vinken M. Primary hepatocyte cultures for liver disease modeling. Curr Opin Toxicol. 2021;25:1-5. https://doi.org/10.1016/j.cotox.2020.08.003 DOI: https://doi.org/10.1016/j.cotox.2020.08.003
Goodla L, Manubolu M, Pathakoti K, Poondamalli PR. Preventive and curative effects of Cocculus hirsutus (Linn.) Diels leaves extract on CCl4 provoked hepatic injury in rats. Egypt J Basic Appl Sci. 2017;4(4):264-9. https://doi.org/10.1016/j.ejbas.2017.10.004 DOI: https://doi.org/10.1016/j.ejbas.2017.10.004
Oke GO, Abiodun AA, Imafidon CE, Monsi BF. Zingiber officinale (Roscoe) mitigates CCl4-induced liver histopathology and biochemical derangements through antioxidant, membrane-stabilizing and tissue-regenerating potentials. Toxicol Rep. 2019;6:416-25. https://doi.org/10.1016/j.toxrep.2019.05.001 PMid:31193041 DOI: https://doi.org/10.1016/j.toxrep.2019.05.001
Yamakawa Y, Doi T, Naitou Y, Kawai H, Mitsumoto A, Kudo N, et al. A single pretreatment with clofibric acid attenuates carbon tetrachloride-induced necrosis, but not steatosis, in rat liver. Food Chem Toxicol. 2020;145:111591. https://doi.org/10.1016/j.fct.2020.111591 DOI: https://doi.org/10.1016/j.fct.2020.111591
Rashidi N, Khatibjoo A, Taherpour K, Mohammad AG, Shirzadi H. Effects of licorice extract, probiotic, toxin binder and poultry litter biochar on performance, immune function, blood indices and liver histopathology of broilers exposed to aflatoxin-B1. Poult Sci. 2020;99(11):5896-906. https://doi.org/10.1016/j.psj.2020.08.034 DOI: https://doi.org/10.1016/j.psj.2020.08.034
Li M, Zhou Y, Zuo L, Nie D, Li X. Dietary fiber regulates intestinal flora and suppresses liver and systemic inflammation to alleviate liver fibrosis in mice. Nutrition. 2021;81:110959. https://doi.org/10.1016/j.nut.2020.110959 PMid:33059126 DOI: https://doi.org/10.1016/j.nut.2020.110959
Galani BR, Owona BA, Chuisseu DP, Machewere E, Ngantchouko CB, Moundipa PF. Hepatoprotective activity of Leptadenia hastata (Asclepiadaceae) on acetaminophen-induced toxicity in mice: In vivo study and characterization of bioactive compounds through molecular docking approaches. Biomed Res Int. 2020;2020:3807234. https://doi.org/10.1155/2020/3807234 PMid:32953880 DOI: https://doi.org/10.1155/2020/3807234
Youssef FS, Ashour ML, Sobeh M, El-Beshbishy HA, Singab AN, Wink M. Eremophila maculata-isolation of a rare naturally-occurring lignan glycoside and the hepatoprotective activity of the leaf extract. Phytomedicine. 2016;23(12):1484-93. https://doi.org/10.1016/j.phymed.2016.08.006 PMid:27765369 DOI: https://doi.org/10.1016/j.phymed.2016.08.006
Kumar PP, Krishnaswamy G, Desai NR, Sreenivasa S, Kumar DB. Design, synthesis, PASS prediction, in-silico ADME and molecular docking studies of substituted-(Z)-3-benzylidine-5-aza-2-oxindole derivatives (Part-1). Chem Data Collect. 2021;31:100617. https://doi.org/10.1016/j.cdc.2020.100617 DOI: https://doi.org/10.1016/j.cdc.2020.100617
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Copyright (c) 2021 Erlintan Sinaga, Syafruddin Ilyas, Salomo Hutahaean, Panal Sitorus (Author)
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