Hepatoprotective Activity of Pirdot Leaves (Saurauia vulcani Korth) Ethanol Extract in Laboratory Rats (Rattus norvegicus) and Characterization of Bioactive Compounds Using a Molecular Docking Approach

Erlintan Sinaga; Syafruddin Ilyas; Salomo Hutahaean; Panal Sitorus

doi:10.3889/oamjms.2021.7624

Authors

Erlintan Sinaga Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
Syafruddin Ilyas Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
Salomo Hutahaean Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
Panal Sitorus Department of Pharmacy, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7624

Keywords:

Pirdot leaves, Serum glutamic pyruvic transaminase, Serum glutamic oxaloacetic transaminase, Cyclooxygenase-2 and tumour necrosis factor alpha

Abstract

AIM: The hepatoprotective activities of bioactive compounds Pirdot were investigated in vivo and in silico.

METHODS: In this study, the completely randomized design non-factorial was experimentally to assess the value of SGPT and SGOT and twenty four adult male rats were divided into four groups : group G₀, control group; group G₁, a treated group received 0.1 ml sheep red blood cell; group G₂, a treated group received 500 mg ethanol extract Pirdot; group G₃, a group treated received 500 mg ethanol extract Pirdot and 0,1 ml sheep red blood cell. On thirty one days of treatment, the blood of all rats group were taken to value SGPT and SGOT using DiaLab kit. Furthermore, the molecular docking study was done to analyse molecular interaction that COX-2 and TNF-α were the primary target protein of bioactive compounds of Pirdot associated with hepatoprotective activities. In addition, it tends to be the target of non-steroidal anti-inflammatory drugs such as Ibuprofen.

RESULTS: The results show SGOT and SGPT value significantly [p<0.05] decreased on Group G₂ and G₃. Moreover, the bioactive compounds of Pirdot, such as Pomolic acid and Ursolic acid tend to be the potential compound on liver protection. Moreover, Pomolic acid has a good binding affinity -14.6 kcal mol^-1 with COX-2 Protein and the binding affinity of cis-3-O-p-hydroxycinnamoyl Ursolic acid was -15.1 kcal mol^-1 associated with TNF-α Protein.

CONLUSION: Pirdot Leaves (Saurauia vulcani Korth.) Ethanol Extract showed Hepatoprotective activity in rats (Rattus norvegicus). Molecular docking approach showed that pomolic acid has a good binding affinity with COX-2 Protein and TNF-α Protein.

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