Effect of the Ethanol Extract of Mimosa Leaves on the Blood Glucose, Malondialdehyde, and Histopathological Characteristics of Wistar Rats

S. Wahjuni; I. A. Raka Astiti Asih; Desmon Tutu Bili; Ni Made Puspawati; Ahmad Fudholi

doi:10.3889/oamjms.2021.7641

Authors

S. Wahjuni Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Jimbaran, Bali, Indonesia
I. A. Raka Astiti Asih Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Jimbaran, Bali, Indonesia
Desmon Tutu Bili Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Jimbaran, Bali, Indonesia
Ni Made Puspawati Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Jimbaran, Bali, Indonesia
Ahmad Fudholi Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Selangor, Malaysia; National Research and Innovation Agency Republic of Indonesia (BRIN), Bandung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7641

Keywords:

Mimosa pudica leaves, Antihyperglycemia test, Histopathology

Abstract

BACKGROUND: Hyperglycemia, a condition characterized by high blood glucose levels, is one of the hallmarks of type 2 diabetes mellitus.

AIM: This study aimed to (1) determine if the ethanol extract of Mimosa leaves could lower blood glucose levels in Wistar rats with hyperglycemia and (2) identify the components of active compounds with antihyperglycemic properties.

METHODS: In the blood glucose level reduction test, Wistar rats were used as an animal model of diabetes induced by streptozotocin. Blood glucose levels were measured using a glucometer, and active compounds were identified through Liquid Chromatography with tandem mass spectrometry (LCMS/MS).

RESULTS: Results showed that the ethanol extract of Mimosa pudica leaves might significantly lower the blood glucose, and malondialdehyde levels of rats with hyperglycemia at 150 mg/kg BB (p < 0.05). LCMS/MS analysis revealed that the ethanol extract of Mimosa leaves likely contained seven compounds that could decrease blood glucose and malondialdehyde levels: DL-valine, indoline, 2-quinolinol, trans-anrthole, luteolin, apigenin, linoliamide, and luteolinin.

CONCLUSION: Histophatological determination with HE staining for pancreas.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Erguder BI, Avci A, Devrim E, Durak I. Effects of cooking techniques on antioxidant enzyme activities of some fruits and vegetables. Turk J Med Sci. 2007;37(3):151-6.

Banik G, Bawari M, Dutta CM, Choudhury S, Sharma GD. Some antidiabetic plants of Southern Assam, India. J Sci Technol. 2010;5:114-9.

Azmi L, Singh MK, Akhtar AK. Pharmacological and biological overview on Mimosa pudica Linn. Int J Pharm Life Sci. 2011;2(11):1226-34.

Tamilarasi T, Ananthi T. Phytochemical analysis and anti microbial activity of Mimosa pudica Linn. Res J Chem Sci. 2012;2(2):72-4.

Pocock SJ. Clinical Trial a Practical Aproach. New York: A Willey Medical Publication; 2008.

Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: An overview. Indian J Med Res. 2007;125:451-472. PMid:17496368

Gabriel EI, Uneojo OV, Chukwudi E. Evaluation of methanol extract of Gongronema latifolium leaves singly and in combination with glibenclamide for anti-hyperglycemic effects in alloxan-induced hyperglycemic rats. J Intercult Ethnopharmacol. 2014;3(3):119-22. https://doi.org/10.5455/jice.20140610054950 PMid:26401359 DOI: https://doi.org/10.5455/jice.20140610054950

Malik B. Pengaruh Frekuensi Pemberian Jus Sargassum Sp. Terhadap Penurunan Kadar Glukosa Darah Dan Peningkatan Tnf-α Pada Mata Dan Otak Tikus Diabetes Mellitus Tipe 2. Doctoral Dissertation. Malang, Indonesia: Universitas Brawijaya; 2018.

Ghorbani A, Rashidi R, Shafiee-Nick R. Flavonoids for preserving pancreatic beta cell survival and function: A mechanistic review. Biomed Pharmacother. 2019;111:947-57. https://doi.org/10.1016/j.biopha.2018.12.127 PMid:30841474 DOI: https://doi.org/10.1016/j.biopha.2018.12.127

Karim F, Susilawati S, Oswari LD, Fadiya F, Nadya N. Uji aktivitas penghambatan Enzim-glucosidase ekstrak air dan ekstrak etanol kayu kuning (Arcangelisia flava). J Kedokteran dan Kesehatan. 2021;8(1):53-60. DOI: https://doi.org/10.32539/V8I1.13118

Mustapa K, Rizky A, Jura MR. Pengaruh ekstrak tanaman putri malu (Mimosa pudica Linn) terhadap penurunan kadar glukosa darah pada mencit (Mus musculus). J Akademika Kimia. 2017;6(1):7-14. DOI: https://doi.org/10.22487/j24775185.2017.v6.i1.9222

Rosmiati M, Nurjanah KA, Suantika G, Putra RE. Application of compost produced by bioconversion of coffee husk by black soldier fly larvae (Hermetia illucens) as solid fertilizer to lettuce (Lactuca sativa var. crispa): Impact to growth. Proc Int Conf Green Technol. 2017;8(1):38-44.

Prameswari OM, Widjanarko SB. Uji efek ekstrak air daun pandan wangi terhadap penurunan kadar glukosa darah dan histopatologi tikus diabetes mellitus. J Pangan dan Agroindustri. 2013;2(2):16-27.

Kurniasari FT. Pengaruh Ekstrak Metanol Akar Putri Malu (Mimosa pudica L) Pada Kadar Malondialdehidea (MDA) Serum Tikus Wistar (Rattus Norvegicus) Jantan Diabetik Induksi Streptozotocin. Indonesia: Universitas Brawijaya; 2013

Wulan W, Yudistira A, Rotinsulu H. Uji aktivitas antioksidan dari ekstrak etanol daun Mimosa pudica Linn. menggunakan metode DPPH. Pharmacon. 2019;8(1):106-13. DOI: https://doi.org/10.35799/pha.8.2019.29243