Identification of Sarcoptes scabiei by Clinical Examination and Follow-up Examination in Medan City, Indonesia


  • Afrida Aryani Nasution Master Program in Tropical Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Imam Budi Putra Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
  • Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia



Dermoscopy, Microscopy, Polymerase chain reaction, Sarcoptes scabiei


Background: Scabies is a disease caused by the mite Sarcoptes scabiei var. hominis. In Indonesia, scabies ranks third out of the 12 most common skin diseases. In terms of disease screening, direct visualization of dermatitis from mites and microscopy of skin scrapings is less sensitive. PCR and dermoscopy examinations have a high sensitivity value to Sarcoptes mites.

Aims: This study aims to identify Sarcoptes scabiei mites between clinical symptoms and supporting examinations, namely PCR and dermoscopy methods.

Methods: This research is a cross-sectional study, with descriptive analytics. The number of samples of 50 people who met the inclusion criteria was examined by microscopic examination, dermoscopy, and PCR. We state it to be positive if we found scabies mites or their eggs on microscopy, delta wing sign, or jet wet contrail on dermoscopy and there is a 135bp DNA band on PCR.

Results: 50 samples diagnosed with scabies based on cardinal sign of scabies, gender were 80% male and 20% female with an average age of 14 years. Based on the location of the rash, the most rashes were between the fingers and toes, each 26% and the least on the head as much as 2%. Based examination tools, no Sarcoptes scabiei mites were found through microscopic and dermoscopic examination, while the PCR examination found 12 positive samples of scabies.

Conclusion: PCR examination is very sensitive and specific even in very small quantities, with the fore primer SSUDF and the reverse primer SSUDR. Further research is needed to assess the sensitivity and specificity of dermoscopy and PCR in diagnosing scabies.


Download data is not yet available.


Metrics Loading ...

Plum Analytics Artifact Widget Block


Patterson JW. Arthropod-induced disease. In: Weedon’s Skin Pathology. 4th ed. United States: Elsevier; 2016. p. 767-80.

World Health Organization. Neglected Tropical Disease. Geneva: World Health Organization; 2015.

Hay RJ, Steer AC, Engelman D, Walton S. Scabies in the developing world-its prevalence, complications, and management. Clin Microbiol Infect. 2012;18(4):313-23. PMid:22429456 DOI:

Mading M, Sopi IP. Kajian aspek epidemiologi skabies pada manusia. J Penyakit Bersumber Binatang. 2015;2(2):9-18. DOI:

Ratnasari AF, Sungkar S. Prevalensi skabies dan faktor-faktor yang berhubungan di pesantren x jakarta timur. J UI. 2014;2(1):3177. DOI:

Sutejo IR, Rosyidi VA, Zaelany AI. Prevalensi, karakteristik dan faktor-faktor yang berhubungan dengan penyakit skabies di pesantren nurul qarnain kabupaten jember (the prevalence, characteristic and factors of scabies in pesantren nurul qarnain jember). J Pustaka Kesehatan. 2017;5(1):30-4. DOI:

Engelman D, Steer AC. Diagnosis, treatment, and control of scabies: Can we do better? Lancet Infect Dis. 2018;18(8):822-3. PMid:30068496 DOI:

Wong SS, Poon RW, Chau S, Wong SC, To KK, Cheng VC, et al. Development of conventional and real-time quantitative PCR assays for diagnosis and monitoring of scabies. J Clin Microbiol. 2015;53(7):2095-102. PMid:25903566 DOI:

Dupuy A, Dehen L, Bourrat E, Lacroix C, Benderdouche M, Dubertret L, et al. Accuracy of standard dermoscopy for diagnosing scabies. J Am Acad Dermatol. 2007;56(1):53-62. PMid:17190621 DOI:

Kurniati K, Zulkarnain I, Listiawan MY. Kesesuaian gambaran klinis patognomonis infestasi skabies dengan kepositifan pemeriksaan dermoskop dan kerokan kulit (the concordance of clinical pathognomonic manifestation of scabies with positivity of dermoscop and skin scraping). Berkala Ilmu Kesehatan Kulit Kelamin. 2014;26(1):14-21. DOI:

Walter B, Heukelbach J, Fengler G, Worth C, Hengge U, Feldmeier H. Comparison of dermoscopy, skin scraping, and the adhesive tape test for the diagnosis of scabies in a resource-poor setting. Arch Dermatol. 2011;147(4):468-73. PMid:21482897 DOI:

Alasaad SA, Min AM, Paquetti M, Alagaili AN, Amelio S, Berilli F, et al. Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei. Parasites Vectors. 2015;8(1):1-7. PMid:26568063 DOI:

Marghoob AA, Usatine RP. Dermoscopy. In: Dermatologic and Cosmetic Procedures in Office Practice. Philadelphia, PA: Elsevier; 2012. p. 384-403.




How to Cite

Nasution AA, Putra IB, Sari MI. Identification of Sarcoptes scabiei by Clinical Examination and Follow-up Examination in Medan City, Indonesia. Open Access Maced J Med Sci [Internet]. 2021 Dec. 6 [cited 2024 Jul. 21];9(B):1633-6. Available from:

Most read articles by the same author(s)

1 2 3 > >>