Identification of Sarcoptes scabiei by Clinical Examination and Follow-up Examination in Medan City, Indonesia

Afrida Aryani Nasution; Imam Budi Putra; Mutiara Indah Sari

doi:10.3889/oamjms.2021.7692

Authors

Afrida Aryani Nasution Master Program in Tropical Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0003-3803-7311
Imam Budi Putra Department of Dermatology and Venereology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Mutiara Indah Sari Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7692

Keywords:

Dermoscopy, Microscopy, Polymerase chain reaction, Sarcoptes scabiei

Abstract

Background: Scabies is a disease caused by the mite Sarcoptes scabiei var. hominis. In Indonesia, scabies ranks third out of the 12 most common skin diseases. In terms of disease screening, direct visualization of dermatitis from mites and microscopy of skin scrapings is less sensitive. PCR and dermoscopy examinations have a high sensitivity value to Sarcoptes mites.

Aims: This study aims to identify Sarcoptes scabiei mites between clinical symptoms and supporting examinations, namely PCR and dermoscopy methods.

Methods: This research is a cross-sectional study, with descriptive analytics. The number of samples of 50 people who met the inclusion criteria was examined by microscopic examination, dermoscopy, and PCR. We state it to be positive if we found scabies mites or their eggs on microscopy, delta wing sign, or jet wet contrail on dermoscopy and there is a 135bp DNA band on PCR.

Results: 50 samples diagnosed with scabies based on cardinal sign of scabies, gender were 80% male and 20% female with an average age of 14 years. Based on the location of the rash, the most rashes were between the fingers and toes, each 26% and the least on the head as much as 2%. Based examination tools, no Sarcoptes scabiei mites were found through microscopic and dermoscopic examination, while the PCR examination found 12 positive samples of scabies.

Conclusion: PCR examination is very sensitive and specific even in very small quantities, with the fore primer SSUDF and the reverse primer SSUDR. Further research is needed to assess the sensitivity and specificity of dermoscopy and PCR in diagnosing scabies.

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