von Willebrand Factor Gene Polymorphism in Preeclampsia Pregnant at Medan, Indonesia

Dewi Indah Sari Siregar; Muhammad Fidel Ganis Siregar; Gontar Alamsyah  Siregar; Syah Mirsya Warli

doi:10.3889/oamjms.2021.7732

Authors

Dewi Indah Sari Siregar Departement of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Muhammad Fidel Ganis Siregar Departement of Obstetrics and Gynecology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia https://orcid.org/0000-0002-2702-4091
Gontar Alamsyah Siregar Departement of Internal Medicine, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Syah Mirsya Warli Departement of Urology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2021.7732

Keywords:

von Willebrand factor, Polymorphism, Preeclampsia, Pregnant

Abstract

BACKGROUND: von Willebrand Factor (vWF) is a large glycoprotein mediating hemostasis and thrombosis. The roles of vWF are platelets adhesion to sites of vascular damage and stabilization of coagulation factor VIII.

AIM: This study aimed to analyze the polymorphism of the vWF gene on preeclampsia (PE) in pregnancy in Medan, Indonesia.

MATERIALS AND METHODS: DNA was amplified using the polymerase chain reaction and was electrophoresed in agarose 2%. Electrophoresis results were detected using Gel Doc 1000 (Biorad, USA). The sequencing method was used to identify polymorphism from vWF gene.

RESULTS: From 50 samples of PE patients, the g.93308C>T vWF gene polymorphism was found with the percentage of TT, CT, and CC genotypes as 50%, 42%, and 8%, respectively.

CONCLUSION: The c.93308C>T vWF gene polymorphism was found in the genotype percentage of homozygous TT, and heterozygote CT was greater than wild-type CC.

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