von Willebrand Factor Gene Polymorphism in Preeclampsia Pregnant at Medan, Indonesia
DOI:
https://doi.org/10.3889/oamjms.2021.7732Keywords:
von Willebrand factor, Polymorphism, Preeclampsia, PregnantAbstract
BACKGROUND: von Willebrand Factor (vWF) is a large glycoprotein mediating hemostasis and thrombosis. The roles of vWF are platelets adhesion to sites of vascular damage and stabilization of coagulation factor VIII.
AIM: This study aimed to analyze the polymorphism of the vWF gene on preeclampsia (PE) in pregnancy in Medan, Indonesia.
MATERIALS AND METHODS: DNA was amplified using the polymerase chain reaction and was electrophoresed in agarose 2%. Electrophoresis results were detected using Gel Doc 1000 (Biorad, USA). The sequencing method was used to identify polymorphism from vWF gene.
RESULTS: From 50 samples of PE patients, the g.93308C>T vWF gene polymorphism was found with the percentage of TT, CT, and CC genotypes as 50%, 42%, and 8%, respectively.
CONCLUSION: The c.93308C>T vWF gene polymorphism was found in the genotype percentage of homozygous TT, and heterozygote CT was greater than wild-type CC.Downloads
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Copyright (c) 2021 Dewi Indah Sari Siregar, Muhammad Fidel Ganis Siregar, Gontar Alamsyah Siregar, Syah Mirsya Warli (Author)
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