The Superior Outcome of Collagen Nanosilver in Increasing Macrophage and Collagen on Deep Dermal Burn Wound Healing

Anggun Ari Mukti; Awal Prasetyo; Yan Wisnu Prajoko; Najatullah Najatullah; Neni Susilaningsih

doi:10.3889/oamjms.2022.7779

Authors

Anggun Ari Mukti Department of General Surgery, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Awal Prasetyo Department of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Yan Wisnu Prajoko Department of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Najatullah Najatullah Department of Biomedical Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
Neni Susilaningsih Department of Plastic Surgery, Faculty of Medicine, Diponegoro University, Semarang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.7779

Keywords:

Collagen, Macrophages, Wound healing, Silver, Anti-inflammatory agents

Abstract

AIM: This study aims to prove the superior outcome of collagen nanosilver in increasing macrophage and collagen on deep dermal burn wound healing.

METHODS: This study was a randomized control trial design. All animals used were male Sprague Dawley rats aged 12–14 weeks old and weighed about 200–250 g. Each rat gets four site injuries and is given treatments. The treatment group includes: I (negative control using NaCl 0.9%), II (positive control using silver sulfadiazine cream), III (treatment with nanosilver collagen cream 1%), and IV (treatment with collagen nanosilver cream 2%). Macrophages counts were assessed using IHC F40/80 and collagen density was assessed using Masson’s trichrome staining.

RESULTS: Macrophage on day 6 showed a significant difference as seen in group III and group IV. A significant difference was also obtained between macrophage on day 3 and day 6 from group IV, likewise between collagen between day 3 and day 6 from group III and IV. Collagen nanosilver acts as an antimicrobial and anti-inflammatory agent by increasing the mechanism of activation and migration of macrophages to the site of injury. Moreover, this formula modulated the collagen deposition, which forms the tissue fibers which ultimately provide a conducive environment for wound healing.

CONCLUSION: Collagen nanosilver has a superior outcome in the increasing macrophage cell count and collagen density of deep dermal burn wound healing.

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