Microhardness and Fracture Resistance of Radicular Dentin Treated with Different Concentrations of Calcium Hydroxide in Endodontic Regeneration Procedures

Sara N. Hashem; Maha Adel Elhousiny

doi:10.3889/oamjms.2021.7803

Authors

Sara N. Hashem Department of Pedodontics and Oral Health, Alazhar University for Girls, Cairo, Egypt https://orcid.org/0000-0002-8611-7650
Maha Adel Elhousiny Department of Endodontics, Alazhar University for Girls, Cairo, Egypt

DOI:

https://doi.org/10.3889/oamjms.2021.7803

Keywords:

Revascularization, Calcium hydroxide, Microhardness, Fracture resistance, Radicular dentin

Abstract

Purpose: to reveal the effect of different concentrations of prepared calcium hydroxide pastes (70%, 50%, and 30%) used in regenerative endodontic on microhardness and fracture resistance of radicular dentin. Material and methods: Different concentrations of calcium hydroxide (Ca(OH)₂) were prepared with measured pH, then forty eight single rooted teeth were prepared and randomized into three groups according to Ca(OH)₂ paste concentrations (12 samples each) and 12 samples were availed as control group. Group I: root canal contained 30% Ca(OH)₂ paste. Group II: root canal contained 50% Ca(OH)₂ paste. Group III: root canal contained 70% Ca(OH)₂ paste. Samples were stored at 37 ⁰C with 100% humidity for four weeks for subsequent microhardness and fracture resistance tests. Results: There was a statistically significant reduction of microhardness and fracture resistance between test groups and control group (P≤ 0.05), group III showed a significant reduction in both microhardness and fracture resistance compared to group I. However, there was no significant difference in pH between different concentration of Ca(OH)₂. Conclusion: Increasing concentration of Ca(OH)₂ paste can negatively affect microhardness and fracture resistance of radicular dentin in revascularization procedure.

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References

Abd El Bary MK, El Shafei JM, Roshdy NN. Comparison of the effect of calcium hydroxide nanoparticles paste versus double antibiotic paste used in regenerative endodontic procedure on the chemical structure of radicular dentin (an in vitro study). Acta Sci Dent Sci. 2019;3(8):1-9. https://doi.org/10.31080/asds.2019.03.0608 DOI: https://doi.org/10.31080/ASDS.2019.03.0608

Dominguez Reyes A, Munoz LM, Martın TA. Study of calcium hydroxide apexification in 26 young permanent incisors. Dent Traumatol. 2005;21:141-5. https://doi.org/10.1111/j.1600-9657.2005.00289.x DOI: https://doi.org/10.1111/j.1600-9657.2005.00289.x

Torabinejad M, Nosrat A, Verma P, Udochukwu O. Regenerative endodontic treatment, or mineral trioxide aggregate apical plug in teeth with necrotic pulps and open apices: A systematic review and meta-analysis. J Endod. 2017;43(11):1806-20. https://doi.org/10.1016/j.joen.2017.06.029 PMid:28822564 DOI: https://doi.org/10.1016/j.joen.2017.06.029

Antunes LS, Salles AG, Gomes CC, Andrade TB, Delmindo MP, Antunes LA. The effectiveness of pulp revascularization in root formation of necrotic immature permanent teeth: A systematic review. Acta Odontol Scand. 2016;74(3):161-9. https://doi.org/10.3109/00016357.2015.1069394 PMid:26179397 DOI: https://doi.org/10.3109/00016357.2015.1069394

American Association of Endodontists: Considerations for Regenerative Procedures; 2018. Available from: http://www.aae.org/clinical-resources/regenerative-endodontics/considerations-for-regenerative-procedures.aspx [Last accessed on 2018 Jan 04].

Mallishery S, Shah T. Regenerative endodontics-looking inward. J Adv Med Med Res. 2020;32(7):83-98. https://doi.org/10.9734/jammr/2020/v32i730454 DOI: https://doi.org/10.9734/jammr/2020/v32i730454

Yassen GH, Eckert GJ, Platt JA. Effect of intracanal medicaments used in endodontic regeneration procedures on microhardness and chemical structure of dentin. Restor Dent Endod. 2015;40(2):104-12. https://doi.org/10.5395/rde.2015.40.2.104 PMid:25984471 DOI: https://doi.org/10.5395/rde.2015.40.2.104

de Rossi A. Effect of rotary or manual instrumentation, with or without a calcium hydroxide/1% chlorhexidine intracanal dressing, on the healing of experimentally induced chronic periapical lesions. Oral Surg Oral Med Oral Pathol Oral Radiol Endodontol. 2005;5:628-36. https://doi.org/10.1016/j.tripleo.2004.07.018 PMid:15829889 DOI: https://doi.org/10.1016/j.tripleo.2004.07.018

Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod. 2012;38(10):1372-5. https://doi.org/10.1016/j.joen.2012.06.018 PMid:22980180 DOI: https://doi.org/10.1016/j.joen.2012.06.018

Yassen G, Platt J. The effect of non setting calcium hydroxide on root fracture and mechanical properties of radicular dentine: A systematic review. Int Endod J. 2013;46(2):112-8. https://doi.org/10.1111/j.1365-2591.2012.02121.x PMid:22970899 DOI: https://doi.org/10.1111/j.1365-2591.2012.02121.x

Eymirli S, Eymirli A. The effect of intracanal medication variations on microhardness of simulated immature root dentin. Aust Endod J. 2021 Dec;47(3):616-623. https://doi.org/10.1111/aej.12532 PMid:34062622 DOI: https://doi.org/10.1111/aej.12532

Kahler W, Lin L. A review of regenerative endodontics: Current protocols and future directions. J Istanb Univ Fac Dent. 2017;51 Suppl 3:S41-51. https://doi.org/10.17096/jiufd.53911. PMid:29354308 DOI: https://doi.org/10.17096/jiufd.53911

Hawkins JJ, Torabinejad M, Li Y, Retamozo B. Effect of three calcium hydroxide formulations on fracture resistance of dentin over time. Dent Traumatol. 2015;31(5):380-4. https://doi.org/10.1111/edt.12175 PMid:25891936 DOI: https://doi.org/10.1111/edt.12175

Kahler SL, Shetty S, Andreasen FM, Kahler B. The effect of long-term dressing with calcium hydroxide on the fracture susceptibility of teeth. J Endod. 2018;44(3):464-9. https://doi.org/10.1016/j.joen.2017.09.018 PMid:29254817 DOI: https://doi.org/10.1016/j.joen.2017.09.018

Salem-Milani A, Zand V, Asghari-Jafarabadi M, Zakeri- Milani P, Banifatemeh A. The effect of protocol for disinfection of extracted teeth recommended by center for disease control (CDC) on microhardness of enamel and dentin. J Clin Exp Dent. 2015;7(5):e552-6. https://doi.org/10.4317/jced.52280 PMid:26644828 DOI: https://doi.org/10.4317/jced.52280

Athanassiadis B, Walsh L. Aspects of solvent chemistry for calcium hydroxide medicaments. Materials. 2017;10(10):1219. DOI: https://doi.org/10.3390/ma10101219

Prather BT, Ehrlich Y, Spolnik K, Platt JA, Yassen GH. Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentine. J Oral Sci. 2014;56(4):245-51. https://doi.org/10.2334/josnusd.56.245 PMid:25500921 DOI: https://doi.org/10.2334/josnusd.56.245

Topçuoğlu HS, Kesİm B, Düzgün S, Tuncay Ö, Demİrbuga S, Topçuoğlu G. The effect of various backfilling techniques on the fracture resistance of simulated immature teeth performed apical plug with biodentine. Int J Paediatr Dent. 2015;25(4):248-54. https://doi.org/10.1111/ipd.12137 PMid:25263283 DOI: https://doi.org/10.1111/ipd.12137

Yassen GH, Vail MM, Chu TG, Platt JA. The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentine. Int Endod J. 2013;46(7):688-95. https://doi.org/10.1111/iej.12046 PMid:23331240 DOI: https://doi.org/10.1111/iej.12046

Teoh YY, Athanassiadis B, Walsh LJ. The influence of aqueous and PEG 400 solvent vehicles on hydroxyl ion release from calcium hydroxide medicaments. Int Dent. 2016;11:42-50.

Shahdad SA, McCabe JF, Bull S, Rusby S, Wassell RW. Hardness measured with traditional vickers and martens hardness methods. Dent Mater. 2007;23(9):1079-85. https://doi.org/10.1016/j.dental.2006.10.001 PMid:17141860 DOI: https://doi.org/10.1016/j.dental.2006.10.001