Cell-free DNA in Human Follicular Fluid as Biomarker for Intracytoplasmic Sperm Injection Procedure Outcome

Maanee Azzam; Adeela Hamood; Hind Abdulkadim

doi:10.3889/oamjms.2021.7810

Authors

Maanee Azzam Department of Gynecology, Kamal Al-Samari hospital, Ministry of health of Iraq, Baghdad, Iraq
Adeela Hamood Department of Gynecology, Ministry of Health, Local Arab Council for Health Specializations, Baghdad, Iraq
Hind Abdulkadim Department of Urosurgery and Infertility, College of Medicine, University of Kufa, Kufa, Iraq

DOI:

https://doi.org/10.3889/oamjms.2021.7810

Keywords:

Cell-free DNA, Human follicular fluid, Intracytoplasmic sperm injection, Procedure outcome

Abstract

Background: Follicular fluid considered as an important microenvironment for oocyte development, cell free-DNA (cfDNA) fragments that are found in this fluid and are released from cell apoptosis and/or necrosis, aimed to quantified the level of cf-DNA, in the follicular fluid and to assess any relation between the level of cf-DNA in this fluid with women’s age, duration of infertility, cause of infertility, her ovarian reserve values. Methods: Eighty-nine women were prospectively included in this study FF cf-DNA which was determined by conventional real time PCR-syber green detection approach which quantified by ALU-specific primers. Results: cell-free DNA (cfDNA) level in Follicular fluid samples of Iraqi women level was; cfDNA (Mean±SD, 0.916±0.106 ng/μl). there was no significant relation between cfDNA and pregnancy outcome, but very low level and very high level cf DNA were related to negative pregnancy outcome, cfDNA was second most important predictive factor of pregnancy outcome after fertilization rate, but both not statistically significant p value was (0.622 and 0.241) respectively. Conclusion: current study notice that cfDNA in the follicular fluid may mainly reflect the cellular activity and the balance between programed apoptosis and cell necrosis.

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