Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases


  • Sunarti Sunarti Department of Medical Science, Doctoral Program of Medicine, Prima Indonesia University, Medan, Indonesia
  • Chrismis Novalinda Ginting Department of Biomolecular, Faculty of Medicine, Prima Indonesia University, Medan, Indonesia
  • Sahna Ferdinand Ginting Department of Clinical Pathology, Faculty of Medicine, Prima Indonesia University, Medan, Indonesia



Inulinase, Mold isolation, Fructose, Red dahlia tuber


BACKGROUND: Dahlia tuber as the source of isolates in endophytic mold produces Inulinases. Inulinase is used in industry to produce inulin to become fructose through an enzymatic reaction. Fructose from natural substances constitutes low calories used as diet in diabetes mellitus type 2 patients. An important phase is done obtain the inulinase enzyme through mold isolation.

AIM: The objective of this research was to isolate and identify the molecular of endophytic mold from red dahlia tuber which had the optimum inulinase activity.

METHODS: Mold isolation was done through the stage of surface sterilization and purification. Mold isolation which had the highest inulinase activity was identified in a molecular using the polymerase chain reaction technique with the Internal transcribed spacers ITS region. DNA amplification used primer ITS 1 and ITS 4.

RESULTS: The result of the study showed that there was five-mold isolation with the result of screening high inulinase activity found in UD3 (++), UD4 (++), and UD5 (+++), the value of inulinase enzyme activity of UD3 was 0.582716049, UD4 was 0.330864198, and UD5 was 0.685185185. The result of identifying UD5 molecular in eight DNA tapes was successfully amplified with 660 pb. The result of identifying molecular based on BLAST analysis found Schizophillum commune species

CONCLUSION: It was found that the highest inulinase activity was the species S. commune, which added new variants of inulinase enzyme from red dahlia tuber which could be used by industry to yield fructose through an enzymatic reaction.


Download data is not yet available.


Metrics Loading ...

Plum Analytics Artifact Widget Block


Singh RS, Singh T, Larroche C. Biotechnological applications of inulin-rich feedstocks. Bioresour Technol. 2018;273:641-53. PMid:30503580 DOI:

Ilgın M, Germec M, Turha T. Inulinase production and mathematical modeling from carobextract by using Aspergillus niger. Biotehnol Prog. 2020;36(1):e2919. PMid:31581350 DOI:

Surti A, Mhatre S. Optimization of inulinase production by a fungal species isolated from rotten garlic samples. J Appl Biotechnol. 2021;8(2):164-217.

Singh RS, Chauhan K, Pandey A, Larroch C. Review biocatalytic strategies for the production of high fructose syrup from inulin. Bioresour Tehnol. 2018;260:395-403. PMid:29636277 DOI:

Mohan A, Flora B, Girhar M. Inulinase: An Important Microbial Enzyme in Food Industry. Berlin, Heidelberg: Springer Nature Singapore Pvt Ltd.; 2018. p. 237-48. DOI:

Wan X, Guo H, Liang Y, Zhou C, Liu Z, Li K, et al. The physiological functions and pharmaceutical applications of inulin: A review. Carbohydr Polym. 2020;246:116589. DOI:

Singh RS, Chauhan K, Kaur K, Pandey A. Statistical optimization of solid-state fermentation for the production of fungal inulinase from apple pomace. Bioresour Technol Rep. 2020;9:100364. DOI:

Sing RS, Singh T, Pandey A. Fungal endoinulinase production from raw Asparagus inulin for the production of fructooligosaccharides. Bioresour Technol Rep. 2020;10:100417. DOI:

Paul I, Kumar G. Fungal biofactories as potential inulinase sources for production of fructooligosaccharides. Ch. 15. In: New and Future Developments in Microbial Biotechnology and Bioengineering. Amsterdam, Netherlands: Elsevier; 2020. p. 183-210.

Othman Z, Selim A, Bayoumy S, Saber W. Inulinase production from plant materials by some local yeast strains. J Agric Chem Biotechnol. 2020;11:71-7. DOI:

Taleb KA, Amin SA, Ahmed AI. Production of exo-inulinase from Aspergillus niger and Candida oleophila for degradation of chicory root inulin and ethanol production Middle East J Agric Res. 2019;8(3):855-67.

Kusmiyati N, Sunarti S, Wahyuningsih TD, Widodo W. Inulinase activity of extracellular protein of Lactobacillus casei AP in different growth conditions. Key Eng Mater. 2020;840:101-6. DOI:

Silvera D, Luthfin I, Aulia A, Wahyu PN, Saryono S. Optimization of process parameters for inulinase production from endophytic fungi Fusarium solani LBKURCC67, Humicola fuscoatra LBKURCC68 and Fusarium oxysporum LBKURCC69. Res J Chem Environ. 2018;22(2):70-8.

Ali S, Khalid SW. Kinetic and parametric optimization for the enhanced production of a novel fungal exo-inulinase under liquid cultur. Pak J Zool. 2020;52(5):1657-64. DOI:

Ruswandi, Oktavia B, Azhar M. Determination of fructose levels resulting from inulin hydrolysis with DNS as an oxidizer. J Eksakta. 2018;19(1):14-23. DOI:

Romadhoni RP, Purbaningtias TE, Muhaimin, Fauzi’ah L. Determination of Reduction Sugar form Banana (Musa acuminata Musa balbisiana Colla) with Different Cooking Process by UV-Visible Spectrophotometer. Proceeding ISCE; 2017. p. 403-9.

Hasyyati SN, Suprihadi A, Raharjo B, Dwiatmi K. Isolation and characterization of endophytic fungi from pegagan (Centella asiatica (L.) Urban). J Biol. 2017;6(2):66-74.

Suraya N, Piska F, Pratiwi NW, Ardhi A, Saryono A. Molecular Reidentification of Endophytic Fungus LBKURCC40 Isolated from Dahlia’s Tuber (Dahlia variabilis) Using the Sequence of ITS rDNA. IOP Conference Series: Materials Science and Engineering; 2019. p. 1-8. DOI:

Saryono S, Rakhmana S, Rahayu F, Ardhi A, Pratiwi WN, Titania T. Molecular identification of endophytic fungi isolated from the tuber of Dahlia variabilis and exploration of their ability in producing ß galactosidase. Biodiversitas. 2017;18(1):145-52. DOI:

National Center for Biotechnology Information. BLAST: Basic Local Alignment Search Tool. National Center for Biotechnology Information, US National Library of Medicine; 2021.

Singh RS, Singh RP, Inulinases. Current Developments in Biotechnology and Bioengineering. Amsterdam, Netherlands: Punjabi University: Elsevier Science and Technology; 2017. p. 423-46. DOI:

Risky FU, Wijanarka, Pujiyanto S. Isolation of the yeast producing enzyme inulinase from cherry fruits (Muntingia calabura) and the effect of manganese micronutrients (Mn) on enzyme production. NICHE J Trop Biol. 2019;2(2):27-37.

Saryono, Fitriani, Ukun R, Soedjanaatmadja. Several microorganisms that produce inulinase enzymes, isolation and characterization of enzymes from Aspergillus flavus Gmn11.2 local strains. Chim Nat Acta. 2016;4(3):165-74. DOI:

Debnath S, Saha AK, Panna D. Biological activities of Schizophyllum commune Fr: A wild edible mushroom of Tripura, North East India. J Mycopathol Res. 2017;54(4):469-75.

Ma’riffattullah A, Azhar, M, Iryani. Determination of inulinase activity on inulin substrate A1-Kg from mesophilic bacteria rhizosphere Dahlia tuber (Dahlia sp). MENARA Ilmu. 2019;13(2):153-61.

Rawat HK, Soni H, Suryawanshi RK, Choukade R, Prajapati BP, Kango N. Exo-inulinase production from Aspergillus fumigatus NFCCI 2426: Purification, characterization, and immobilization for continuous fructose production. J Food Sci. 2021;86(5):1778-90. PMid:33884619 DOI:

Paul I, Kumar CG. Fungal biofactories as potential inulinase sources for production of fructooligosaccharides. In: New and Future Developments in Microbial Biotechnology and Bioengineering. India: Elsevier Science and Technology; 2020. p. 183-210. DOI:




How to Cite

Sunarti S, Ginting CN, Ginting SF. Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases. Open Access Maced J Med Sci [Internet]. 2022 Jan. 3 [cited 2023 Dec. 1];10(A):49-55. Available from:

Most read articles by the same author(s)

1 2 > >>