Isolation and Molecular Identification of Endophytic Mold Schizophillum commune in Red Dahlia (Dahlia sp. L) Tuber as Producing Inulinases

Sunarti Sunarti; Chrismis Novalinda Ginting; Sahna Ferdinand Ginting

doi:10.3889/oamjms.2022.7906

Authors

Sunarti Sunarti Department of Medical Science, Doctoral Program of Medicine, Prima Indonesia University, Medan, Indonesia https://orcid.org/0000-0001-8138-4533
Chrismis Novalinda Ginting Department of Biomolecular, Faculty of Medicine, Prima Indonesia University, Medan, Indonesia
Sahna Ferdinand Ginting Department of Clinical Pathology, Faculty of Medicine, Prima Indonesia University, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.7906

Keywords:

Inulinase, Mold isolation, Fructose, Red dahlia tuber

Abstract

BACKGROUND: Dahlia tuber as the source of isolates in endophytic mold produces Inulinases. Inulinase is used in industry to produce inulin to become fructose through an enzymatic reaction. Fructose from natural substances constitutes low calories used as diet in diabetes mellitus type 2 patients. An important phase is done obtain the inulinase enzyme through mold isolation.

AIM: The objective of this research was to isolate and identify the molecular of endophytic mold from red dahlia tuber which had the optimum inulinase activity.

METHODS: Mold isolation was done through the stage of surface sterilization and purification. Mold isolation which had the highest inulinase activity was identified in a molecular using the polymerase chain reaction technique with the Internal transcribed spacers ITS region. DNA amplification used primer ITS 1 and ITS 4.

RESULTS: The result of the study showed that there was five-mold isolation with the result of screening high inulinase activity found in UD3 (++), UD4 (++), and UD5 (+++), the value of inulinase enzyme activity of UD3 was 0.582716049, UD4 was 0.330864198, and UD5 was 0.685185185. The result of identifying UD5 molecular in eight DNA tapes was successfully amplified with 660 pb. The result of identifying molecular based on BLAST analysis found Schizophillum commune species

CONCLUSION: It was found that the highest inulinase activity was the species S. commune, which added new variants of inulinase enzyme from red dahlia tuber which could be used by industry to yield fructose through an enzymatic reaction.

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