Correlation between Gut Microbiota, its Metabolic Products, and their Association with Liver Enzymes among Sample of Egyptian Females

Nayera E. Hassan; Sahar A. El-Masry; Ayat Nageeb; Mohamed S. El Hussieny; Aya Khalil; Manal Mouhamed Aly; Mahmoud A. S.  Afify; Ahmed Ismail; Gamila El-Saeed; Adel Hashish; Mohamed Selim

doi:10.3889/oamjms.2022.7909

Authors

Nayera E. Hassan Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Sahar A. El-Masry Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Ayat Nageeb Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt https://orcid.org/0000-0003-0260-0395
Mohamed S. El Hussieny Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Aya Khalil Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt https://orcid.org/0000-0003-2727-0126
Manal Mouhamed Aly Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Mahmoud A. S. Afify Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Ahmed Ismail Department of Biological Anthropology, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Gamila El-Saeed Department of Medical Biochemistry, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt
Adel Hashish Department of Children with Special Needs, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt https://orcid.org/0000-0001-9549-2973
Mohamed Selim Department Researches and Applications of Complementary Medicine, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.7909

Keywords:

Gut microbiota, Obesity, Liver enzymes, Egyptian females

Abstract

BACKGROUND: Gut microbiota plays critical role in pathogenesis of obesity, liver metabolism and associated diseases.

AIM: The present study aimed to identify existing gut microbiota enterotypes and their metabolic products profiles, and investigate correlation between gut microbiota; body mass index (BMI); and liver enzymes among sample of normal weight and obese Egyptian females.

METHODS: A case-control cross-sectional study included 112 Egyptian females; 82 obese and 30 normal weights; aged 25–60 years. For each participant, anthropometric measurements (weight, height, and BMI), laboratory investigations (Aspartate amino transferase [AST], Alanine amino transferase [ALT], Short-chain fatty acids [SCFA], and C-reactive protein [CRP]) and microbiota analysis were done.

RESULTS: Obese females had higher significant values of CRP, AST, ALT, SCFA, log Bacteroidetes, Firmicutes, Firmicutes/Bacteroidetes ratio, and Lactobacillus. Among normal weight group, Lactobacillus had significant positive correlations with SCFA, Bifidobacteria, and Firmicutes, and significant negative correlations with AST, ALT, and CRP. While Bifidobacteria had significant negative correlations with height and ALT, Bacteroidetes bacteria had significant positive correlations with SCFA, and significant negative correlations with age and height, Firmicutes bacteria had significant negative correlations with AST and ALT, and Firmicutes/Bacteroidetes ratio had significant negative correlations with AST, ALT, and SCFA. Among obese group, Lactobacillus and Bifidobacteria had significant negative correlations with Firmicutes/Bacteroidetes ratio, and Firmicutes bacteria had significant negative correlations with ALT.

CONCLUSION: The beneficial Lactobacillus and Bifidobacteria have their good impact on improving obesity status and liver functions.

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