Effect of Addition of Jelly and Storage Time on the Number of Lactic Acid Bacteria in Yoghurt Processed Products

Authors

  • Retno Martini Widhyasih Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Jakarta III, Bekasi, Indonesia https://orcid.org/0000-0003-0148-8215
  • Jusuf Kristianto Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Jakarta III, Bekasi, Indonesia
  • Lutfi Rahmawati Lubis Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Jakarta III, Bekasi, Indonesia
  • Mega Mirawati Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Jakarta III, Bekasi, Indonesia
  • Atik Khodikoh Department of Nursing, Health Polytechnic Ministry of Health Bandung, Bandung, Indonesia
  • Rahmi Susanti Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Jakarta III, Bekasi, Indonesia
  • Gurid PE Mulyo Department of Nutrition, Health Polytechnic Ministry of Health Bandung, Bandung, Indonesia https://orcid.org/0000-0003-4701-9588

DOI:

https://doi.org/10.3889/oamjms.2021.7916

Keywords:

Yoghurt, Lactic Acid Bacteria, Time of Storage, Jelly Concentrations

Abstract

BACKGROUND: Yoghurt is a milk fermentation product that is one of the highly nutritious food materials involving bacteria. Yoghurt is formed from lactic acid bacteria (LAB), namely, Lactobacillus bulgaricus, Lactobacillus acidophilus and Streptococcus thermophilus. The bacteria are beneficial for health because it creates a balance of the intestinal microflora. Moreover, yoghurt has a distinctive freshness, aroma and taste, namely, sour. In the yoghurt fermentation process, LAB needed a substrate for their growth. The content of natural sugar in milk is minimal; therefore, jelly needs to be added, which the microorganism can directly use for its growth.

AIM: This study aims to determine the effect of adding jelly with various concentrations and storage time on the number of LAB in processed yoghurt products.

METHODS: This research used the measurement method of total LAB with plate count method using completely randomized design factorial pattern 1 × 3 with three repetitions. The factor I is the jelly concentration (0.5%, 0.75% and 1%) and factor II is storage time (7 days, 14 days, 21 days at a temperature of 2–8°C) with data processing using statistical tests, namely, two-way analysis of variance test followed by Duncan’s Multiple Range Test.

RESULTS: The results showed significant differences in the storage time of 7 days, 14 days, and 21 days on agar with concentrations of 0.5%, 0.75%, and 1%. The highest LAB amount was on the 7th day with a concentration of 1% with 2.51 × 109. While on the 21st day, with a concentration of 1%, it had the lowest LAB amount of 3.2 × 108. However, the amount of LAB was still above the Indonesian National Standard, namely >107 CFU/mL.

CONCLUSION: This study concluded that the highest number of LAB was found in the addition of 1% jelly with a storage time of 7 days with a total LAB yield of 2.51 × 109 CFU/mL. Storage time on day 7, day 14 and day 21 and jelly concentrations of 0.5%, 0.75%, and 1% added to processed yoghurt had a significant effect (p < 0.05) on the total number of LAB (p < 0.05) LAB).

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References

Tambunan AR. Characteristics of Probiotics of Various Types of Lactic Acid Bacteria (LAB) in Pineapple Juice Lactate Fermented Drinks. Indonesia: Lampung University; 2016.

Hasfah A. The Effect of Starter Variations on the Quality of Cow’s Milk Yoghurt. Makassar: Alauddin State Islamic University Makassar; 2012.

Widhyasih RM, Lestari E. Effect of Time, Fermentation Temperature, Type of Milk on the Number of Lactic Acid Bacteria and Yoghurt Organoleptic Quality, Final Report of Research Results, Poltekkes Ministry of Health Jakarta III; 2020.

Sandi IM, Bachtiar H, Hidayati. Comparison of the inhibitory effectiveness of curd with yoghurt against the growth of mutant streptococcus bacteria. J B Dent. 2015;2(2):88-94. DOI: https://doi.org/10.33854/JBDjbd.9

Harjantini U, Rustanti N. Total lactic acid bacteria, Ph, and fiber content of srikaya jelly yoghurt functional drink with the addition of carrageenan. J Nutr Coll. 2015;4(2):514-9 Available from: http:// www.ejournal-s1.undip.ac.id/index.php/jnc. [Last accessed on 2021 Oct 20]. https://doi.org/10.14710/jnc.v4i4.10156 DOI: https://doi.org/10.14710/jnc.v4i4.10156

Khaliq A. The Effect of Using Rosella and Adding Different Sugars on Organoleptic Quality and Vitamin C Levels of Rosella (Hibiscus sabdarrifa L.) jelly drink. Semarang: State University of Semarang; 2011.

Widhyasih RM. “Mother Yoghurt” Starter Making Guide Poltekkes Ministry of Health Jakarta III; 2020. Available from: https://www.youtube.com/watch?v=ozx3dnziw3u. [Last accessed on 2021 Oct 21].

National Standardization Body. SNI 2981: 2009 Food and Beverage (Yoghurt Quality Requirements), Jakarta; 2009.

Pranayanti IA, Sutrisno A. Making young coconut water (Cocos nucifera L.) probiotic drink with lactobacillus casei strain shirota as a starter. J Food Agroind. 2015;3(2):763-72.

Wirawati I, Purwani E. Effect of Long Storage at Cold Temperatures On Ph Value, Total Acid and Number of Lactic Acid Bacteria Yoghurt Suweg Flour (Amorphophallus Campanulatus). Indonesia: Muhammadiyah Surakarta University; 2019.

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Published

2021-12-12

How to Cite

1.
Widhyasih RM, Kristianto J, Lubis LR, Mirawati M, Khodikoh A, Susanti R, Mulyo GP. Effect of Addition of Jelly and Storage Time on the Number of Lactic Acid Bacteria in Yoghurt Processed Products. Open Access Maced J Med Sci [Internet]. 2021 Dec. 12 [cited 2024 Mar. 28];9(A):1302-5. Available from: https://oamjms.eu/index.php/mjms/article/view/7916