CK19 and OV6 Expressions in the Liver of 2-AAF/CCl4 Rat Model

Romy Arwinda; Isabella Kurnia Liem; Ayu Eka Fatril; Fanny Oktorina; Firda Asma'ul Husna; Ria Kodariah; Puspita Eka Wuyung

doi:10.3889/oamjms.2022.7923

Authors

Romy Arwinda Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Isabella Kurnia Liem Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Integrated Laboratory, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Ayu Eka Fatril Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Fanny Oktorina Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Firda Asma'ul Husna Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Ria Kodariah Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Puspita Eka Wuyung Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; Animal Research Facilities, Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.7923

Keywords:

Rat model, Liver injury, 2-AAF/CCl4 OV6, CK19

Abstract

BACKGROUND: Liver regeneration on a chronic liver injury with rat model research is urgent to study the pathology of human chronic diseases. 2-Acetylaminofluorene (2-AAF) and Carbon Tetrachloride (CCl₄) can be applied to study about liver regeneration by liver stem cells, known as oval cells. The use of 2-AAF and CCl₄ (2-AAF/CCl₄) to induce chronic liver injury can lead severe hepatocyte damages and extensive fibrosis.

AIM: to observed the OV6 and CK19 expression in the liver of 2AAF/CCl₄ rat model.

METHODS: In this research, a high dose of 2-AAF (10mg/kg) was applied and combined repeatedly with CCl₄ (2ml/kg) for 12 weeks. An immunohistochemistry (IHC) procedure by using OV6 and CK19 antibodies was also applied to examine the regeneration of oval cells. Both antibodies expressions were then examined semi-quantitatively according to the expressions percentage of each sample based on the liver zone. We have observed the OV6 and CK19 expressions in every zone, including in portae hepatis area.

RESULTS: The results showed that OV6 and CK19 with the highest expression in the zone I. A significant difference between OV6 and CK19 expressions was revealed in healthy (control group) and 2-AAF/CCl₄ groups (both indicating p=0.045). Moreover, a ductular reaction was also found in Zone I and II of the 2-AAF/CCl₄.

CONCLUSIONS: We conclude that 2-AAF/CCl₄ induced chronic rat liver injury model, could be utilized to investigate the oval cells with OV6 and CK19 expression.

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