The Activity of Combination of Ethanol Extract of Artocarpus lacucha Buch.-Ham and Anredera cordifolia Steenis Leaves to Increase Wound Healing Process on NIH-3T3 Cell Line

Nazliniwaty Nazliniwaty; Olivia Avriyanti Hanafiah; Dewi Pertiwi; Mahatir Muhammad; Denny Satria

doi:10.3889/oamjms.2022.8006

Authors

Nazliniwaty Nazliniwaty Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Olivia Avriyanti Hanafiah Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, Indonesia
Dewi Pertiwi Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Mahatir Muhammad Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8006

Keywords:

Cell proliferation, VEGFR-2, Ethanol extract, NIH-3T3, Anredera cordifolia, Artocarpus lacucha

Abstract

AIM: This study aims to determine the effect of the combination of ethanol extract of Artocarpus lacucha Buch.-Ham and Anredera cordifolia Steenis leaves to increase cell proliferation and increase VEGFR-2 expression of NIH-3T3.

METHODS: The samples used were Artocarpus lacucha Buch.-Ham and Anredera cordifilia Steenis leaves. The powder of simples was extracted using ethanol 80% with maceration method. The effect of extract combination on proliferation was evaluated using the MTT method. Wound healing assay was established by a cell migration method, and VEGFR-2 expression was determined using RT-PCR.

RESULTS: The effect of combination of ethanol extract of Artocarpus lacucha leaves (EEALL) and ethanol extract of Anredera cordifolia leaves (EEACL) on cell proliferation after 24h, 48h and 72h incubation found as viable cells were showed (124.33 ± 0.32%; 128.52 ± 0.41%; 118.35 ± 0.22%). Percent of wound closed after 24 h and 48 h incubation are 64.88 ± 0.90% and 100.00 ± 0.00%, and expression of VEGFR-2 increased from 1 (control) to 1.58 ± 0.02.

CONCLUSION: The results suggest that a combination of EEALL and EEACL (37.5 μg/mL−37.5 μg/mL) is effective in increasing cells proliferation and hence wound healing process.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Stamm A, Reimers K, Strauß S, Vogt P, Scheper T, Pepelanova I. In vitro wound healing assays-state of the art. BioNanoMat. 2016.17(1-2):79-87. https://doi.org/10.1515/bnm-2016-0002 DOI: https://doi.org/10.1515/bnm-2016-0002

Rodrigues HG, Vinolo MA, Sato FT, Magdalon J, Kuhl CM. Oral administration of linoleic acid induces new vessel formation and improves skin wound healing in diabetic rats. PLoS One. 2016;11(10):1-19. https://doi.org/10.1371/journal.pone.0165115 PMid:27764229 DOI: https://doi.org/10.1371/journal.pone.0165115

Ridiandries A, Bursill C, Tan J. Broad-spectrum inhibition of the CC-chemokine class improves wound healing and wound angiogenesis. Int J Mol Sci. 2017;18(1):155. https://doi.org/10.3390/ijms18010155 PMid:28098795 DOI: https://doi.org/10.3390/ijms18010155

Futagami A, Ishizaki M, Fukuda Y, Kawana S, Yamanaka N. Wound healing involves induction of cyclooxygenase-2 expression in rat skin. Lab Investig. 2002;82(11):1503-13. https://doi.org/10.1097/01.lab.0000035024.75914.39 PMid:12429810 DOI: https://doi.org/10.1097/01.LAB.0000035024.75914.39

Ariffin NH, Hasham R. Potential dermatological application on Asian plants. Biotechnol Bioproc Eng. 2016;21:337-54. https://doi.org/10.1007/s12257-015-0750-4 DOI: https://doi.org/10.1007/s12257-015-0750-4

Jagtap UB, Bapat VA. Artocarpus: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2010;129(2):142-66. https://doi.org/10.1016/j.jep.2010.03.031 PMid:20380874 DOI: https://doi.org/10.1016/j.jep.2010.03.031

Gautam P, Patel R. Artocarpus lakoocha Roxb: An overview. Eur J Complement Alternat Med. 2007;1(1):10-1.

Astuti SM, Sakinah AM, Andayani BM, Risch A. Deter-mination of saponin compound from Anredera Cordifolia (Ten) stennis plant (binahong) to potential treatment for several diseases. J Agric Sci. 2011;3(4):224-32. https://doi.org/10.5539/jas.v3n4p224 DOI: https://doi.org/10.5539/jas.v3n4p224

Kaur G, Utami NV, Usman HA. Effect of topical application of binahong (Anredera cordifolia (Ten.) steenis leaf paste in wound healing process in mice. Althea Med J. 2014;1(1):6-11. https://doi.org/10.15850/amj.v1n1.289 DOI: https://doi.org/10.15850/amj.v1n1.289

Yuliani SH, Fudholi A, Pramono S, Marchaban. The effect of formula to physical properties of wound healing gel of etha- nolic extract of binahong (Anredera cordifolia (Ten.) steenis). Int J Pharm Sci Res. 2012;3(11):4254-9. https://doi.org/10.1063/1.4958594 DOI: https://doi.org/10.1063/1.4958594

Dalimunthe A, Hasibuan PA, Silalahi J, Sinaga SF, Satria D. Antioxidant activity of alkaloid compounds from Litsea cubeba Lour. Orient J Chem. 2018;34(2):1149-52. https://doi.org/10.13005/ojc/340270 DOI: https://doi.org/10.13005/ojc/340270

Satria D, Silalahi J, Haro G, Ilyas S. Hasibuan PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of Picria fel-terrae Lour. herbs. Asian Pac J Cancer Prev. 2017;18(2):399-403. https://doi.org/10.5220/0008359701900193 PMid:28345821 DOI: https://doi.org/10.5220/0008359701900193

Handayani S, Ratna AS, Riris IJ, Meiyanto E. Two active compounds from Caesalpinia sappan L. in combination with cisplatin synergistically induce apoptosis and cell cycle arrest on WiDr cells. Adv Pharm Bull. 2017;7(3):375-80. https://doi.org/10.15171/apb.2017.045 PMid:29071219 DOI: https://doi.org/10.15171/apb.2017.045

Hasibuan PA, Harahap U, Sitorus P, Satria D. Ethylacetate extract of Zanthoxylum acanthopodium DC. fruit against doxorubicin-resistanced T47D cells. Der Pharma Chem. 2016;8(20):172-174. https://doi.org/10.3889/oamjms.2019.178 DOI: https://doi.org/10.3889/oamjms.2019.178

Mao P, Ershao Z, Yang C, Likun L, Daqing R. Pinus massoniana bark extract inhibits migration of the lung cancer A549 cell line. Oncol Lett. 2017;13:1019-23. https://doi.org/10.3892/ol.2016.5509 PMid:28356994 DOI: https://doi.org/10.3892/ol.2016.5509

Harahap U, Hasibuan PA, Sitorus P, Arfian N, Satria D. Antimigration activity of an ethylacetate fraction of Zanthoxylum acanthopodium DC. fruits in 4T1 breast cancer cells. Asian Pac J Cancer Prev. 2018;19(2):565-9. https://doi.org/10.32734/idjpcr.v2i2.3220 PMid:29481017 DOI: https://doi.org/10.32734/idjpcr.v2i2.3220

Harahap U, Hasibuan PA, Sitorus P, Satria D. Cytotoxicity activity of Picria Fel-Terrae Lour. Herbs against 4T1 and MCF-7 breast cancer cells. Asian J Pharm Clin Res. 2018;11(1):194-5. https://doi.org/10.22159/ajpcr.2018.v11s1.26608 DOI: https://doi.org/10.22159/ajpcr.2018.v11s1.26608

Hasibuan PA, Harahap U, Sitorus P, Satria D. The anticancer activities of Vernonia amygdalina Delile. Leaves on 4T1 breast cancer cells through phosphoinositide-3-kinase (PI3K pathway). Heliyon. 2020;6:e04449. https://doi.org/10.1016/j.heliyon.2020.e04449 DOI: https://doi.org/10.1016/j.heliyon.2020.e04449

Freiesleben SH, Soelberg J, Nyberg NT, Jäger AK. Determination of the wound healing potentials of medicinal plants historically used in Ghana. Evid Based Complement Alternat Med. 2017;2017:9480791. https://doi.org/10.1155/2017/9480791 DOI: https://doi.org/10.1155/2017/9480791

Ebeling S, Naumann K, Pollok S, Wardecki T, Vidal S, Nascimento JM, et al. From a traditional medicinal plant to a rational drug: Understanding the clinically proven wound healing efficacy of birch bark extract. PLoS One. 2014;9(1):e86147. https://doi.org/10.1371/journal.pone.0086147 PMid:24465925 DOI: https://doi.org/10.1371/journal.pone.0086147

Sofyanti E, Siregar DI, Siregar O, Boel T, Satria D, Tann G. Identification of single nucleotide polymorphism insulin-like growth factor type 1 gene in vertical mandibular asymmetry patients with idiopathic scoliosis symptom. Asian J Pharm Clin Res. 2018;11(1):100-3 https://doi.org/10.22159/ajpcr.2018.v11s1.26686 DOI: https://doi.org/10.22159/ajpcr.2018.v11s1.26686

Kuonen R, Weissenstein U, Urech K, Kunz M, Hostanska K, Estko M, et al. Effects of lipophilic extract of Viscum album L. and oleanolic acid on migratory activity of NIH/3T3 fibroblasts and on hacat keratinocytes. Evid Based Complement Alternat Med. 2013;2013:718105. https://doi.org/10.1155/2013/718105 PMid:24379890 DOI: https://doi.org/10.1155/2013/718105

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in wound healing: A comprehensive review. Adv Wound Care. 2014;3(7):445-64. https://doi.org/10.1089/wound.2013.0473 PMid:25032064 DOI: https://doi.org/10.1089/wound.2013.0473

Liang CC, Park AY, Guan JL. In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007;2:329. https://doi.org/10.1038/nprot.2007.30 DOI: https://doi.org/10.1038/nprot.2007.30

Bryan N, Ahswin H, Smart N, Bayon Y, Wohlert S, Hunt JA. Reactive oxygen species (ROS)-a family of fate deciding molecules pivotal in constructive inflammation and wound healing. Eur Cell Mater. 2012;24:e65. https://doi.org/10.22203/ecm.v024a18 DOI: https://doi.org/10.22203/eCM.v024a18

Chandrasekharan NV, Dai H, Roos KL, Evanson NK, Tomsik J, Elton TS, et al. COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: Cloning, structure, and expression. PNAS. 2002;99(21):13926-31. https://doi.org/10.1073/pnas.162468699 PMid:12242329 DOI: https://doi.org/10.1073/pnas.162468699

Jamuna S, Pulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J App Pharm Sci. 2012;2:149-54. https://doi.org/10.7324/japs.2012.2722 DOI: https://doi.org/10.7324/JAPS.2012.2722

Nagmoti DM, Khatri DK, Juvekar PR, Juvekar AR. Antioxidant activity free radical-scavenging potential of Pithecellobium dulce Benth seed extracts. Free Radic Antioxid. 2012;2(2):37-43. https://doi.org/10.5530/ax.2012.2.2.7 DOI: https://doi.org/10.5530/ax.2012.2.2.7

Rosidah, Yam MF, Sadikun A, Asmawi MZ. Antioxidant potential of Gyunura procumbens. Pharm Bio. 2008;46:616-25. https://doi.org/10.1080/13880200802179642 DOI: https://doi.org/10.1080/13880200802179642

Silalahi J, Rosidah, Yuandani, Satria D. Virgin coconut oil modulates TCD4+ and TCD8+ cell profile of doxorubicin-induced immune-suppressed rats. Asian J Pharm Clin Res. 2018;11(1):37-8. https://doi.org/10.22159/ajpcr.2018.v11s1.25562 DOI: https://doi.org/10.22159/ajpcr.2018.v11s1.25562

Koleva II, Van Beek TA, Linssen JP, de Groot A, Evstatieva LN. Screening of plant extracts for antioxidant activity: A comparative study on three testing methods. Phytochem Anal. 2002;13(1):8-17. https://doi.org/10.1002/pca.611 PMid:11899609 DOI: https://doi.org/10.1002/pca.611

Li X, Kong L, Liao S, Lu J, Ma L, Long X. The expression and significance of feces cyclooxygensae-2 mRNA in colorectal cancer and colorectal adenomas. Saudi J Gastroenterol. 2017;23(1):28-34. https://doi.org/10.4103/1319-3767.199112 PMid:28139497 DOI: https://doi.org/10.4103/1319-3767.199112

Auliafendri N, Rosidah, Yuandani, Suryani S, Satria D. The immunomodulatory activities of picria fel-terrae lour herbs towards RAW 264.7 Cells. Open Access Maced J Med Sci. 2019;7(1):24-8. https://doi.org/10.3889/oamjms.2019.017 PMid:30740154 DOI: https://doi.org/10.3889/oamjms.2019.017

Silalahi J, Surbakti C. Burn wound healing activity of hydrolyzed virgin coconut oil. Int J PharmTech Res. 2015;8(1):67-73.

Khuanekkapham M, Chanal N, Warachate K. Anti-aging potential and phytochemicals of Centella asiatica, Nelumbo nucifera, and Hibiscus sabdariffa extracts. J Adv Pharm Technol Res. 2020;11(4):174-8. https://doi.org/10.4103/japtr.japtr_79_20 PMid:33425700 DOI: https://doi.org/10.4103/japtr.JAPTR_79_20