Olive Polyphenol as Neuroprotective in Chronic Cervical Myelopathy Rabbit Model

Sabri Ibrahim; Iqbal Fahlevi Adeputra Nasution; Mahyu Danil; Wismaji Sadewo; Tri Widyawati; Putri Chairani Eyanoer; Ridha Dharmajaya; Kiking Ritarwan; Wibi Riawan; Michael Lumintang Loe; Tommy Rizky Hutagalung

doi:10.3889/oamjms.2022.8009

Authors

Sabri Ibrahim Philosophy Doctor in Medicine Programme, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Iqbal Fahlevi Adeputra Nasution Department of General Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Mahyu Danil Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Wismaji Sadewo Department of Neurosurgery, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia
Tri Widyawati Department of Pharmacology and Therapeutic, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Putri Chairani Eyanoer Department of Community Health Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Ridha Dharmajaya Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Kiking Ritarwan Department of Neurology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Wibi Riawan Department of Biochemistry and Biology Molecular, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Michael Lumintang Loe Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Tommy Rizky Hutagalung Department of Neurosurgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8009

Keywords:

Olive leaf extract, Oleuropein, Olive polyphenol, Neurofilaments, S-100, Brain derived neurotrophic facto, Chronic cervical myelopathy, Neuroprotective

Abstract

BACKGROUND: Olive polyphenols are known to be an anti-oxidants and anti-inflammatory agents.

AIM: The purpose of this study was to determine the potential neuroprotective effect on chronic cervical myelopathy rabbit model.

METHODS: This study was divided into six groups; control negative (Sham-Operated) group, control positive 1 and 2, treatment groups 1, 2, and 3. Olive leaf extract (OLE) gives 350 mg/kg BW and spinal cord sample was taken at the compression level C5. Histopathological assessment and immunohistochemistry of neurofilaments (NF), S-100, brain derived neurotrophic factor (BDNF), and evaluation of functional motoric outcome were done before animals were terminated.

RESULTS: Chronic cervical myelopathy in rabbit model causes decreased expression of NF, S-100, BDNF, and decreased motor function. Oral administration of OLE increased the expression of these biomarkers and improved motor function outcomes.

DISCUSSION: These findings indicate that OLE may be effective in protecting chronic cervical myelopathy in rabbit model.

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