Effect of SHBG Polymorphism on the Levels of Bioavailable Testosterone and Lipid Metabolism in Older Men of the Kazakh Population

Merkhat Akkaliyev; Nurlan Aukenov; Meruyert Massabayeva; Bakytbek Apsalikov; Saule Rakhyzhanova; Muratkhan Kuderbaev; Nariman Sadykov

doi:10.3889/oamjms.2022.8145

Authors

Merkhat Akkaliyev Department of Surgical Disciplines, Semey Medical University, Semey, Kazakhstan https://orcid.org/0000-0003-3122-7411
Nurlan Aukenov Department of Health and Human Resources, Ministry of Health of the Republic of Kazakhstan
Meruyert Massabayeva Department of Surgical Disciplines, Semey Medical University, Semey, Kazakhstan https://orcid.org/0000-0001-8240-361X
Bakytbek Apsalikov Department of Surgical Disciplines, Semey Medical University, Semey, Kazakhstan https://orcid.org/0000-0001-6983-9224
Saule Rakhyzhanova Department of Surgical Disciplines, Semey Medical University, Semey, Kazakhstan
Muratkhan Kuderbaev Department of Surgical Disciplines, Semey Medical University, Semey, Kazakhstan
Nariman Sadykov Department of Surgery, Pavlodar Branch of the Semey Medical University, Pavlodar, Kazakhstan

DOI:

https://doi.org/10.3889/oamjms.2022.8145

Keywords:

SHBG, Bioavailable testosterone, Hypogonadism, Lipid metabolism disorders in men

Abstract

This study is aimed at investigating the effect of SHBG (rs727428; rs10822184) and LPL (rs754493647) single nucleotide polymorphisms on the concentration of the bioavailable fraction of testosterone in older men.

Materials and methods To study gene mutations, 417 residents of the East Kazakhstan region of Kazakh nationality were examined. The main group included 135 men with signs of hypogonadism (AMS 37-49), and the control group consisted of 282 healthy men (AMS 17-26) of the corresponding age (p = 0.5). Single nucleotide polymorphisms rs 727428 [C / T]; rs10822184 [T / C]; rs754493647 [T / C], was determined by the TaqMan method.

Results Analysis of the rs727428 polymorphism has revealed that the TT allele (rs727428) has a lower level of albumin (p = 0.03), bioavailable testosterone (p = 0.04), and free testosterone (p = 0.6) than in carriers of the CC and CT genotypes. Also, it has shown a decrease in total testosterone (p = 0.001) and an increase in SHBG levels (p = 0.07) in men with the TT genotype of the rs727428 gene polymorphism. The rs10822184 polymorphism demonstrated an increase in triglyceride and LDL levels in TT genotype (p ≤ 0.04), in comparison with CC and CT genotypes.

Conclusion It has been proven that rs727428 (p = 0.001) is associated with testosterone levels and therefore can determine the concentration of bioavailable testosterone. Decreased levels of bioavailable testosterone are a sign of male hypogonadism. This study confirms the effect of rs10822184 on LDL (p = 0.01) and triglyceride (p = 0.04) levels, but its association with androgen levels has not been proven. Our results may be of interest for understanding the etiology of early development of hypogonadism and lipid metabolism disorders in men. To confirm the conclusions, a more detailed study with a large sample of men from the Kazakh population may be required.

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