Effect of Different Solvent on Phytochemical Content, Tyrosinase Inhibition and Antioxidant Activities of Campolay (Pouteria campechiana kunth. [Baehni.])

Sani Nurlaela Fitriansyah; Rika Hartati; Irda Fidrianny

doi:10.3889/oamjms.2022.8204

Authors

Sani Nurlaela Fitriansyah Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia; Department of Pharmaceutical Biology, Indonesian School of Pharmacy (Sekolah Tinggi Farmasi Indonesia), Bandung, Indonesia https://orcid.org/0000-0003-4613-5616
Rika Hartati Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
Irda Fidrianny Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8204

Keywords:

Pouteria campechiana, Tyrosinase, Antioxidant, Quercitrin

Abstract

BACKGROUND: Pouteria campechiana leaves are reported to have phenol and flavonoid compounds. Phenolic and flavonoid compounds can act as tyrosinase inhibitor and antioxidant.

AIM: The purpose of this study was to compare phytochemical content, tyrosinase inhibition, antioxidant activities, and determine of marker compound from P. campechiana leaves extract with different polarities solvent.

METHODS: In addition, the content of marker compound from P. campechaina leaves extract was determined by HPLC.

RESULTS: The highest total phenolic content (TPC) 7.83 GAE/100 g extract, IC50 of tyrosinase 171.512 ± 1.352 and IC50 of DPPH 0.968 ± 0.008 was given by ethanolic extract (DE). Meanwhile ethyl acetate extract (DEA) had the highest of total flavonoid content 2.544 ± 0.554 QEA/100 g extract. The TPC correlated with tyrosinase inhibitory activity and antioxidant activity.

CONCLUSION: Quercitrin was as marker compound from P. campechiana leaves extract, and quercitrin content in the DEA of P. campechiana leaves was 3.539%, while in the DE was 0.153%.

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