Effect of Different Solvent on Phytochemical Content, Tyrosinase Inhibition and Antioxidant Activities of Campolay (Pouteria campechiana kunth. [Baehni.])

Authors

  • Sani Nurlaela Fitriansyah Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia; Department of Pharmaceutical Biology, Indonesian School of Pharmacy (Sekolah Tinggi Farmasi Indonesia), Bandung, Indonesia https://orcid.org/0000-0003-4613-5616
  • Rika Hartati Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
  • Irda Fidrianny Department of Pharmaceutical Biology, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8204

Keywords:

Pouteria campechiana, Tyrosinase, Antioxidant, Quercitrin

Abstract

BACKGROUND: Pouteria campechiana leaves are reported to have phenol and flavonoid compounds. Phenolic and flavonoid compounds can act as tyrosinase inhibitor and antioxidant.

AIM: The purpose of this study was to compare phytochemical content, tyrosinase inhibition, antioxidant activities, and determine of marker compound from P. campechiana leaves extract with different polarities solvent.

METHODS: In addition, the content of marker compound from P. campechaina leaves extract was determined by HPLC.

RESULTS: The highest total phenolic content (TPC) 7.83 GAE/100 g extract, IC50 of tyrosinase 171.512 ± 1.352 and IC50 of DPPH 0.968 ± 0.008 was given by ethanolic extract (DE). Meanwhile ethyl acetate extract (DEA) had the highest of total flavonoid content 2.544 ± 0.554 QEA/100 g extract. The TPC correlated with tyrosinase inhibitory activity and antioxidant activity.

CONCLUSION: Quercitrin was as marker compound from P. campechiana leaves extract, and quercitrin content in the DEA of P. campechiana leaves was 3.539%, while in the DE was 0.153%.

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Published

2022-01-18

How to Cite

1.
Fitriansyah SN, Hartati R, Fidrianny I. Effect of Different Solvent on Phytochemical Content, Tyrosinase Inhibition and Antioxidant Activities of Campolay (Pouteria campechiana kunth. [Baehni.]). Open Access Maced J Med Sci [Internet]. 2022 Jan. 18 [cited 2024 Mar. 5];10(A):158-63. Available from: https://oamjms.eu/index.php/mjms/article/view/8204