Lemon Pepper Extract Improves Diabetic Nephropathy in Diabetic Rats

Authors

  • Linda Chiuman Department of Histology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia https://orcid.org/0000-0003-4822-0099
  • Chrismis Novalinda Ginting Department of Histology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia
  • OK Yulizal Department of Histology, Faculty of Medicine, Universitas Prima Indonesia, Medan, North Sumatera, Indonesia https://orcid.org/0000-0001-7916-9658

DOI:

https://doi.org/10.3889/oamjms.2022.8226

Keywords:

BUN, Creatinine, Lemon Pepper, Diabetic, Nephropathy

Abstract

BACKGROUND: Prolonged uncontrol non-insulin dependent diabetes mellitus (NIDDM) leads to various complications. One of these complications is diabetic nephropathy. Around 24% of the US population suffered from diabetes between 2009 and 2014, and 25% suffered from chronic kidney disease. Lemon pepper fruit may become an alternative treatment from a natural product to prevent kidney damage in diabetic conditions.

AIM: The aim of the study was to investigate the kidney protection effect of lemon pepper extract in streptozotocin-induced Wistar rats with diabetic nephropathy.

METHODS: This experimental study used 25 male Wistar rats grouped into five different groups. These groups were standard, control, lemon pepper ethanol extract (LPEE)-I, II, and III that were received Metformin, 0.5% Na-CMC, and lemon pepper ethanol extract at the dose of 500, 750, and 1000 mg/kg BW, respectively.

RESULTS: The intervention was performed for 14 days. The result showed that the standard, control, lemon pepper ethanol extract (LPEE)-I, II, and III significantly decreased the BUN (Blood urea nitrogen) and reatinine level (p < 0.05). Moreover, the histology study also showed some improvement of like preserved tubular and glomerular from necrosis.

CONCLUSION: The highest lemon pepper ethanol extract dose significantly decreased the BUN (23.08%) and creatinine (59.49%) levels.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Budiharsana MP. Risk differences between elderly men and women toward doctor-diagnosed diabetes mellitus in urban areas in Indonesia: 2013 National Basic Health research data. Kesmas. 2017;12(1):15-21. DOI: https://doi.org/10.21109/kesmas.v12i1.1436

American Diabetes Association. Standards of Medical Care in Diabetes-2020. New York: American Diabetes Association; 2019.

Masruroh E. Hubungan umur dan status gizi dengan kadar gula darah penderita diabetes melitus Tipe II [Relationship between age and nutritional status against blood glucose levels in Type II diabetes mellitus patients]. Journal of Health Sciences. 2018;6(2):153. DOI: https://doi.org/10.32831/jik.v6i2.172

Soelistijo S, Novida H, Rudijanto A, Soewondo P, Suastika K, Manaf A, et al. Konsesus Pengelolaan Dan Pencegahan Diabetes Melitus Tipe 2 Di Indonesia 2015 [Consensus on Management and Prevention of Type 2 Diabetes Mellitus in Indonesia 2015]. Indonesia: Perkeni; 2015. p. 82.

Ningrum AM. Gambaran tingkat pengetahuan penderita diabetes melitus terhadap komplikasi diabetes melitus di kota pontianak [Description of knowledge level in diabetes mellitus patients on complications of diabetes mellitus in Pontianak city]. J MHS PSPD FK Univ Tanjungpura. 2018;3(1):1-13.

Powers AC. Diabetes mellitus: Complications. In: Harrison’s Endocrinology. 4th ed. New York: Mc Graw Hill; 2017. p. 327-38.

Worotikan RV, Tuju EA, Kawuwung F. Analisa efektivitas antidiabetes ekstrak etanol buah andaliman (Zanthoxylum acanthopodium DC) pada histopatologi ginjal tikus putih (Rattus norvergicus) yang diinduksi alloksan [Analysis of anti-diabetic effectiveness of andaliman fruit (Zanthoxylum acanthopodium DC) ethanol extract on alloxan-induced white rat (Rattus norvergicus) kidney histopathology]. Journal of Science, Mathematics and Education. 2017;5(1):29-37.

Winarti W, Simanjuntak P, Syahidin MF. Identifikasi senyawa kimia aktif antioksidan dari ekstrak etil asetat buah andaliman (Zanthoxylum acanthopodium DC) [Identification of antioxidant active chemical compounds from ethyl acetate extract of andaliman fruit (Zanthoxylum acanthopodium DC)]. In: Talenta Conference Series: Tropical Medicine (TM). Medan: Talenta Publisher; 2018. p. 162-6. DOI: https://doi.org/10.32734/tm.v1i3.283

Sitanggang FM, Duniaji AS, Pratiwi ID. Daya hambat ekstrak buah andaliman (Zanthoxylum acanthopodium DC) dalam etil asetat terhadap pertumbuhan Escherichia coli [Inhibitory ability of lemon pepper fruit (Zanthoxylum acanthopodium DC) ethyl acetate extract on Escherichia coli growth]. Journal of Food Science and Technology. 2019;8(3):257-66. DOI: https://doi.org/10.24843/itepa.2019.v08.i03.p04

Yanti PT, Nuriasari N, Juliana K. Lemon pepper fruit extract (Zanthoxylum acanthopodium DC.) suppresses the expression of inflammatory mediators in lipopolysaccharide-induced macrophages in vitro. Am J Biochem Biotechnol. 2011;7(4):190-5. DOI: https://doi.org/10.3844/ajbbsp.2011.190.195

Saragih DE, Arsita EV. The phytochemical content of Zanthoxylum acanthopodium and its potential as a medicinal plant in the regions of Toba Samosir and North Tapanuli, North Sumatra. Pros Semin Nas Masy Biodiversitas Indones. 2019;5(1):71-6.

Ridho M, Lindarto D. The Effect of Andaliman Fruit Extract to Blood Glucose Levels of Mice with Type 1 Diabetes. In: Adella CA, editor. Stem Cell Oncology. Medan: CRC Press; 2017. p. 95-8. DOI: https://doi.org/10.1201/9781351190152-21

Chiuman L, Ginting CN, Yulizal O, Suhartomi, Chiuman V. Improvement of liver function from lemon pepper fruit ethanol extract in streptozotocin-induced wistar Rats. In: 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences (InHeNce). Medan, Indonesia: IEEE; 2021. DOI: https://doi.org/10.1109/InHeNce52833.2021.9537284

Mutia MS, Chiuman L. Efek hepatoprotektif ekstrak etanol Citrus sinensis L. osbeck pada tikus yang diinduksi parasetamol [hepatoprotective effect of Citrus sinensis L. osbeck ethanol extract in paracetamol-induced rats]. Medicine magazine Bandung. 2019;51(4):189-93. DOI: https://doi.org/10.15395/mkb.v51n4.1794

Mutia MS. Histology study of liver changes paracetamol-induced wistar rats treated with sunkist (Citrus sinensis L. Osbeck) extract. Am Sci Res J Eng Technol Sci. 2019;59(1):1-7.

Mutia MS, Ginting CN, Yulizal O. Sunkist peel ethanol extract ameliorates diabetic nephropathy in streptozotocin-induced diabetic wistar rats. Open Access Maced J Med Sci. 2021;9(A):1086-91. DOI: https://doi.org/10.3889/oamjms.2021.7362

Wardhani FM, Chiuman L, Ginting CN, Ginting SF. Role of cystatin-C as serum biomarkers in predicting glomerular function associated with copper-induced acute kidney injury. Medicine magazine Bandung. 2020;52(1):16-21. DOI: https://doi.org/10.15395/mkb.v52n1.1793

Ongko NX, Chiuman L, Ginting CN. Effect of white turmeric rhizome extract (Curcuma zedoaria) on testis histology of male wistar rat. Am Sci Res J Eng Technol Sci. 2019;55(1):69-74.

Sembiring F, Unitly AJ, Eddy L. Effects of clove syrup therapy on creatinine, urea, and kidney histology of diabetes mellitus rat. J Biothecnol Conserv Wallacea. 2021;1(1):31-41.

Kaur N, Kishore L, Singh R. Dillenia indica L. attenuates diabetic nephropathy via inhibition of advanced glycation end products accumulation in STZ-nicotinamide induced diabetic rats. J Tradit Complement Med. 2017;8(1):226-38. https://doi.org/10.1016/j.jtcme.2017.06.004 PMid:29322013 DOI: https://doi.org/10.1016/j.jtcme.2017.06.004

Vijayaraj R, Kumaran NS, Swarnakala. In vivo and in vitro models for biological screening of anti-diabetic drugs. Int J Pharm Sci. 2019;9(2):294-86.

Downloads

Published

2022-01-18

How to Cite

1.
Chiuman L, Ginting CN, Yulizal O. Lemon Pepper Extract Improves Diabetic Nephropathy in Diabetic Rats. Open Access Maced J Med Sci [Internet]. 2022 Jan. 18 [cited 2024 Feb. 26];10(A):170-5. Available from: https://oamjms.eu/index.php/mjms/article/view/8226

Funding data

Most read articles by the same author(s)

Similar Articles

You may also start an advanced similarity search for this article.