Evaluation of the Antibacterial Activity and the Remineralizing Potentiality of Two Natural Extracts and Their Mixture (In Vitro Study)

Reham Saleh; Shaymaa Nagi; Hanaa Elgamily; Shahinaz Hassan; Hadeer El-Hashemy; Mohamed Zaazou

doi:10.3889/oamjms.2022.8228

Authors

Reham Saleh Department of Restorative and Dental Materials, Oral and Dental Research Institute, National Research Centre, Giza, Egypt
Shaymaa Nagi Department of Restorative and Dental Materials, Oral and Dental Research Institute, National Research Centre, Giza, Egypt
Hanaa Elgamily Department of Restorative and Dental Materials, Oral and Dental Research Institute, National Research Centre, Giza, Egypt
Shahinaz Hassan Department of Restorative and Dental Materials, Oral and Dental Research Institute, National Research Centre, Giza, Egypt
Hadeer El-Hashemy Department of Pharmaceutical Technology, National Research Centre, Giza, Egypt
Mohamed Zaazou Department of Restorative and Dental Materials, Oral and Dental Research Institute, National Research Centre, Giza, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.8228

Keywords:

Remineralization, Antibacterial, Eggshell, Ginger, Enamel, White spot lesions

Abstract

AIM: This study aimed to assess the antibacterial activity against the cariogenic Streptococcus mutans (ATCC 25175) and the commensal Actinomyces viscous (1300 EMCCN) oral bacteria and to determine the remineralizing effect of the previously demineralized bovine enamel lesion of ginger extract, eggshell extract as well as their mixture.

METHODS: The two different extracts; eggshell extract and ginger extract were prepared as well as their mixture in an equal volume. Their antibacterial activity against S. mutans and A. viscous was assessed by well diffusion test and clear zones diameters measurement (mm). Chlorhexidine and Dimethyl sulfoxide solution were utilized as positive and negative control, respectively. Thirty-six anterior bovine teeth were subjected to artificial caries pH cycling regimen including alternate demineralization and remineralization for 5 days. Then they were randomly divided into three groups (G) (n = 12): G1: Ginger, G2: Eggshell, and G3: Mixture. Specimens of each group were separately immersed in 50 ml of the tested extract for 12 h seven successive days. Vickers Microhardness and Energy Dispersive X-ray (EDX) Analysis were assessed at baseline, after demineralization and remineralization.

RESULTS: Both extracts had antibacterial effect against the profile growth of S. mutants. However, the mixture showed the highest statistically significant mean inhibition zone diameters (mm), followed by ginger and eggshell extracts with no statistically significant difference between them. Regarding the remineralizing efficacy; G1:(Ginger) group showed the highest statistically significant mean microhardness followed by G3:(Mixture) group, then eggshell treated specimens; which showed the least statistically significant mean microhardness values. Regarding EDIX analysis results, a statistically significant increase in F (wt%) after remineralization in both eggshell and ginger treated specimens.

CONCLUSION: Ginger as well as mixture extracts not only have antibacterial effect against cariogenic bacteria but also have a strong remineralizing ability of demineralized enamel.

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References

Mohamed EO, Sharaf AA, Talaat DM, Nagui DA. Remineralization effect of eggshell powder and novamine on initial caries-like lesions in young permanent teeth (An in vitro study). Alexandria Dent J. 2020;45:52-9. DOI: https://doi.org/10.21608/adjalexu.2020.86761

Abdel Tawab HA, Niazy MA, El Sharkakawy DA. Antibacterial effect of ginger, green tea and pomegranate versus chlorhexidine using stevia and sucrose sugar. Al-Azhar Dent J. 2020;7(1):329-36. http://doi.org/10.21608/adjg.2020.7838.1105 DOI: https://doi.org/10.21608/adjg.2020.7838.1105

Gocmen GB, Yanikoglu F, Tagtekin D, Stookey GY, Schemehom BR, Hayran O. Education effectiveness of some herbals on initial enamel caries lesion. Asian Pac J Trop Biomed 2016;6:846-50. DOI: https://doi.org/10.1016/j.apjtb.2016.08.005

Bilgin G, Yanıkoğlu F, Tagtekin D. Remineralization potential of herbal mixtures: An in situ study. Paripex Indian J Res. 2016;5(2):264-8.

Anushri M, Yashoda R, Manjunath P. Herbs: A good alternatives to current treatments for oral health problems. Int J Adv Health Sci. 2015;1(12):26-32.

Kiristiogglu ZB, Yanikoglu F, Alkan E, Tagtekin D, Gocmen GB, Ilgin C. The effect of dental paste with herbal content on remineralization and the imaging with fluorescent technique in teeth with white spot lesion. Clin Exp Health Sci. 2021;11:348-53. DOI: https://doi.org/10.33808/clinexphealthsci.848455

Taher HM, Bayoumi R. Remineralization of initial enamel like lesions with chicken eggshell powder solution versus amorphous calcium phosphate. Egypt Dent J. 2018;64:3703-12. DOI: https://doi.org/10.21608/edj.2018.79237

Feroz S, Moeen F, Haq SN: Protective effect of two different remineralizing agents on artificially induced dental erosion in primary and permanent teeth: An in vitro analysis. Pak Oral Dent J. 2017;37(4):657-66.

Elshik AE, Etman WM, Genaid TM. Resistance of chicken eggshell powder treated with demineralized enamel to further acidic challenges. Tanta Dent J. 2020;17:38-44. DOI: https://doi.org/10.4103/tdj.tdj_14_19

Alkhalidi EF, Alsalman TH, Taqa AA. Antibacterial properties of new calcium based cement prepared from egg shell. Edorium J Dent. 2015;2:21-7.

Park M, Bae J, Lee D. Antibacterial activity of [10] gingerol and [12]-gingerol isolated from ginger rhizome against periodontal bacteria. Phytother Res J. 2008;22:1446-9. DOI: https://doi.org/10.1002/ptr.2473

Mony B, Ebenezar AV, Ghani MF, Narayanan A, Anands S, Mohan AG. Effect of chicken egg shell powder solution on early enamel carious lesions: An in vitro preliminary study. J Clin Diagn Res. 2015;9(3):ZC30-2. http://doi.org/10.7860/JCDR/2015/11404.5656 PMid:25954701 DOI: https://doi.org/10.7860/JCDR/2015/11404.5656

Feroz S, Moeen F, Haq SN. Protective effect of chicken egg shell powder solution (CESP) on artificially induced dental erosion: An in vitro atomic force microscope study. Int J Dent Sci Res. 2017;5(3):49-55. DOI: https://doi.org/10.12691/ijdsr-5-3-2

Nada M. Mostafa: Antibacterial activity of ginger (Zingiber officinale) leaves essential oil nanoemulsion against the cariogenic Streptococcus mutans. J Appl Pharm Sci. 2018;8(9):34-41. http://doi.org/10.7324/JAPS.2018.8906 DOI: https://doi.org/10.7324/JAPS.2018.8906

El-Sayed SM, El-Sayed HS. Antimicrobial nanoemulsion formulation based on thyme (Thymus vulgaris) essential oil for UF labneh preservation. J Mater Res Technol. 2021;10:1029-41. DOI: https://doi.org/10.1016/j.jmrt.2020.12.073

Espinosa L, Ruiz N, Tarín DC, López SY, Contreras AZ, Cortéz DC, et al. Antiadherence and antimicrobial properties of silver nanoparticles against Streptococcus mutans on brackets and wires used for orthodontic treatments. J Nanomater. 2018;2018:9248527. DOI: https://doi.org/10.1155/2018/9248527

Beatriz BS, Marília FP, Márcio GH, Ricardo SC, Felipe AJ, Ricardo DG, et al. In vitro evaluation of the remineralizing potential and antimicrobial activity of a cariostatic agent with silver nanoparticles. Braz Dent J. 2017;28(6):738-43. DOI: https://doi.org/10.1590/0103-6440201701365

Elgamily H, Ghallab O, El-Sayed H, Nasr M. Antibacterial potency and fluoride release of a glass ionomer restorative material containing different concentrations of natural and chemical products: An in-vitro comparative study. J Clin Exp Dent 2018;10:e312-20. DOI: https://doi.org/10.4317/jced.54606

Zaheer AS, Tanikonda R, Girija S, Madhu V, Kalyan S, Vijayalakshmi BR, et al. Quantitative analysis of remineralization of artificial carious lesions with commercially available newer remineralizing agents using SEM-EDX-in vitro study. J Clin Diagn Res. 2017;11(4):20-3.

Ali N, Shabnam A, Azad R, Elmira N. Impact of Nano hydroxyapatite, Nano silver fluoride and sodium fluoride varnish on primary teeth enamel remineralization: An in vitro study. J Clin Diagn Res. 2017;11(9):97-100.

Rao R, Jain A, Verma M, Langade D, Patil A. Comparative evaluation of remineralizing potential of fluoride using three different remineralizing protocols: An in vitro study. J Conserv Dent. 2017;20(6):463-6. http://doi.org/10.4103/JCD. JCD_203_17 PMid:29430102 DOI: https://doi.org/10.4103/JCD.JCD_203_17

Ambarkova V, Gorseta K, Glavina D, Skrinjaric I. The effect of fluoridated dentifrice formulations on enamel remineralisation and microhardness after in vitro demineralization. Acta Stomatol Croat. 2011;45(3):159-65.

Ambarkova V, Gorseta K, Jankulovska M, Glavina D, Skrinjaric I. Effect of the fluoride gels and varnishes comparing to CPP-ACP complex on human enamel demineralization/remineralization. Acta Stomatol Croat. 2013;47(2):99-110. DOI: https://doi.org/10.15644/asc47/2/1

Mony S, Rao A, Shenoy R, Supraabha BS. Comparative evaluation of the remineralizing efficacy of calcium sodium phosphosilicate agent and fluoride based on quantitative and qualitative analysis. J Indian Soc Pedod Prev Dent. 2015;33(4):291-5. http://doi.org/10.4103/0970-4388.165667 PMid:26381630 DOI: https://doi.org/10.4103/0970-4388.165667

Haleh H, Maryam HG, Yasaman M, Mohamad JK. The effect of two remineralizing agents and natural saliva on bleached enamel hardness. Dent Res J (Isfahan). 2016;13(1):52-7. http://doi.org/10.4103/1735-3327.174713 PMid:26962316 DOI: https://doi.org/10.4103/1735-3327.174713

Chris SI, Timothy LH, Frankling GG. Microhardness recovery of demineralized enamel after treatment with fluoride gel or CPP-ACP paste applied topically or with dielectrophoresis. Am J Dent. 2012;25(2):109-13. PMid:22779285

Chaudhary I, Tripathi AM, Yadav G, Sonali S. Effect of casein phosphopeptide amorphous calcium phosphate and calcium sodium phosphosilicate on artificial carious lesions: An in vitro study. Int J Clin Pediatr Dent. 2017;10(3):261-6. http://doi.org/10.5005/jp-journals-10005-1447 PMid:29104386 DOI: https://doi.org/10.5005/jp-journals-10005-1447

Liang K, Wang S, Tao1 S, Xiao S, Zhou H, Wang P, et al. Dental remineralization via poly (amido amine) and restorative materials containing calcium phosphate nanoparticles. International Journal of Oral Science, 2019, 11-15. DOI: https://doi.org/10.1038/s41368-019-0048-z

Zhang H, Yang J, Liang K, Li J, He L, Yang AX, et al. Effective dentin restorative material based on phosphate-eterminated dendrimer as artificial protein. Colloids Surf B Biointerfaces. 2015;128:304-14. http://doi.org/10.1016/j.colsurfb.2015.01.058 PMid:25703791 DOI: https://doi.org/10.1016/j.colsurfb.2015.01.058

Liang K, Yuan H, Li J, Yang J, Zahu X, He L, et al. Remineralization of demineralized dentin induced by amineterminated PAMAM dendrimer. Macromol Mater Eng. 2015;300(1):107-17. http://doi.org/10.1002/mame.201400207 DOI: https://doi.org/10.1002/mame.201400207

GaoY, Li K, Li J, Yuan H, Liu H, Duan Z, et al. Effect and stability of poly (amido amine) induced biomineralization on dentinal tubule occlusion. Materials (Basel). 2017;10(4):384-99. http://doi.org/10.3390/ma10040384 PMid:28772744 DOI: https://doi.org/10.3390/ma10040384

Muzzalupo R., Tavano L, Cassano R, Trombino S, Ferrarelli T, Picci N. A new approach for the evaluation of niosomes as effective transdermal drug delivery systems. Eur J Pharm Biopharm. 2011;79(1):28-35. http://doi.org/10.1016/j.ejpb.2011.01.020 PMid:21303691 DOI: https://doi.org/10.1016/j.ejpb.2011.01.020

Philip N, Suneja B, Walsh LJ. Ecological approaches to dental caries prevention: Paradigm shift or shibboleth? Caries Res J. 2018;52(1-2):153-65. http://doi.org/10.1159/000484985 PMid:29320767 DOI: https://doi.org/10.1159/000484985

Duggal MS, Curson ME. Dental disease: Etiology of dental caries. In: Encyclopedia of Food Sciences and Nutrition; 2003. p. 1746-9. DOI: https://doi.org/10.1016/B0-12-227055-X/00325-4

Abdo RY, Niazy MA, Gad NA. The remineralizaing potential of some natural materials alone or combined with zamzam water on demineralized enamel. Al Azhar Dent J. 2021;8(1):27-33. DOI: https://doi.org/10.21608/adjg.2020.14199.1184

Haghoo R, Mehran M, Ahmadvand M, Ahmadvand MJ. Remineralization effect of eggshell versus Nano-hydroxyapatite on caries-like lesions in permanent teeth (in vitro). J Int Oral Health. 2016;8(4):435-9. DOI: https://doi.org/10.5005/jp-journals-10024-1905

Onwubu SC, Mduli PS, Singh S, Bharuth V. An in vitro examination on the effectiveness of commercial toothpastes in the prevention of tooth decay, using eggshell as a substitute for human tooth material. South Afr Dent J. 2018;73(7):446-51. DOI: https://doi.org/10.17159/2519-0105/2018/v73no7a3

Klaric E, Rakic M, Sever I, Milat O, Par M, Tarle Z. Enamel and dentin microhardness and chemical composition after experimental light-activated bleaching. J Oper Dent. 2015;40(4):E132-41. http://doi.org/10.2341/14-148-L PMid:25748206 DOI: https://doi.org/10.2341/14-148-L