Dayak Onions (Eleutherine americana L Merr) Reduced Mesothelial Cell Detachment After Laparoscopy in Rats

Authors

  • Hery Poerwosusanta Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
  • Alfi Yasmina Department of Pharmacology, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
  • Firli Rahmah Primula Dewi Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia
  • Krist Nathania Benita Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Angga Setya Budi Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Nanda Setya Putri Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Nur Ainun Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Ghyna Fakhriah Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Anisa Fitri Medical Profession Program, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesi
  • Adam Rahardiyan Poerwosusanta Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
  • Elvira Esmeralda Poerwosusanta Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
  • Donny Aditia Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
  • Zairin Noor Department of Surgery, Faculty of Medicine, Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8297

Keywords:

Eleutherine americana, Inflammation, Oxidative stress, Laparoscopy, Mesothelial cell

Abstract

Background: Laparoscopy induces changes and detachment of mesothelial structure. Studies on the prevention of mesothelial cell detachment are rarely found. The Dayak tribe uses the Dayak onion (Eleutherine americana L. Merr) as a wound-healing agent due to its anti-inflammatory and antioxidant activities. This study aimed to prove the anti-inflammatory and antioxidant activities of Dayak onions in preventing mesothelial cell damage after laparoscopy.

Materials and methods: Thirty male Sprague-Dawley rats were classified into five groups (n = 6 per group), namely: (a) control, (b) Mediclore, (c) Dayak onion, 30-, (d) 60-, and (e) 90 mg/kg body weight, respectively. The transforming growth factor-beta (TGF-β) and total oxidant status in the peritoneal fluid were determined 24 hours after laparoscopy. Histopathological analysis of mesothelial cell numbers and the protein Zone Occludin-1 (ZO-1) expression in the peritoneum, small intestines, greater omentum, and liver were performed 7 days after the procedure. An in-silico study was conducted to analyze the anti-inflammatory effects of the components of Dayak onions.

Results: The in-silico study showed that one of the Dayak onion active compounds, eleutherine, had a potential anti-inflammatory effect and acted as a modulator of TGF-β. Following Dayak onion administration, the TGF- level, the number of mesothelial cell detachments, and ZO-1 expression were all significantly reduced (p<0.05), whereas the total oxidant status (TOS) level was not (p>0.05).

Conclusions: Our study showed that Dayak onion administration reduced TGF-β level, number of mesothelial cell detachment, and ZO-1 expression following laparoscopy.

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2022-01-26

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Poerwosusanta H, Yasmina A, Dewi FRP, Benita KN, Budi AS, Putri NS, Ainun N, Fakhriah G, Fitri A, Poerwosusanta AR, Poerwosusanta EE, Aditia D, Noor Z. Dayak Onions (Eleutherine americana L Merr) Reduced Mesothelial Cell Detachment After Laparoscopy in Rats. Open Access Maced J Med Sci [Internet]. 2022 Jan. 26 [cited 2024 May 1];10(A):1321-9. Available from: https://oamjms.eu/index.php/mjms/article/view/8297

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Vol. 10 No. A (2022): A - Basic Sciences

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Pharmacology

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Copyright (c) 2022 Hery Poerwosusanta, Alfi Yasmina, Firli Rahmah Primula Dewi, Krist Nathania Benita, Angga Setya Budi, Nanda Setya Putri, Nur Ainun, Ghyna Fakhriah, Anisa Fitri, Adam Rahardiyan Poerwosusanta, Elvira Esmeralda Poerwosusanta, Donny Aditia, Zairin Noor (Author)

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