The Evidence-based Effect of Platelet-rich Fibrin in Osteogenesis: A Systematic Review and Meta-analysis

Mujaddid Idulhaq; Ambar Mudigdo; Pamudji Utomo; Brian Wasita

doi:10.3889/oamjms.2022.8309

Authors

Mujaddid Idulhaq Doctoral Program, Faculty of Medicine, Universitas Sebelas Maret, Solo, Indonesia https://orcid.org/0000-0003-0895-248X
Ambar Mudigdo Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Solo, Indonesia
Pamudji Utomo Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Sebelas Maret, Prof. Dr. R. Soeharso Orthopedic Hospital, Solo, Indonesia https://orcid.org/0000-0001-6701-9521
Brian Wasita Department of Anatomical Pathology, Faculty of Medicine, Universitas Sebelas Maret, Solo, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8309

Keywords:

PRF, Platelet-Rich Fibrin, Osteogenesis

Abstract

ABSTRACT

Background: Platelet Rich Fibrin (PRF) is a growth factor concentration functioning as an initiator of wound healing and bone regeneration, and it is mainly reported in dentistry rehabilitation and rejuvenation.

Objective: summarize and analyze the evidence based on the effect of PRF on the bone osteogenesis

Methods: A comprehensive search was conducted on the bibliographic databases or Medical Subject Headings in the PubMed. The search was conducted among articles that were published between 2010 and 2021. Required article information was extracted from each article by abstract and full paper availability that focuses on the Platelet-Rich Fibrin (PRF) And Osteogenesis. We recruited studies with the design was employed clinical trials with in vitro and in vivo approaches. The only study that provided osteogenesis outcome proceeded to the quantitative analysis.

Results: Regarding literature search and screening processes, it yielded 24 studies for qualitative assessment and eleven studies for quantitative analysis. Most of the studies performed a combination of PRF with other materials such as Mg ring, BMSCs, Gold nanoparticles, and PDLSCs. It showed that PRF combined with other materials enhanced the osteogenic ability. The assessment of PRF only showed the various result in multiple outcome markers. For the ALP, the mean difference is 1.40 [1.14-1.67] p-value 0.001. It indicates there is a significant effect of PRF application with the increase of ALP. For the RUNX2, there is a significant effect of PRF application with the increase of RUNX2 1.10 [0.93, 1.26]. For OCN, the mean difference of PRF in OCN is 0.77 [0.43, 1.12] p=0.001. It showed a significant effect of PRF application with the increase of OCN. There is also a significant effect of PRF application for TRAP with the declining number of TRAP is -1.59 [-2.96, -0.22] p=0.001.

Conclusion: PRF combined with other materials showed more promising results rather than PRF only. Moreover, in the assessment of PRF only, it was found that PRF has a significant effect in accelerating bone osteogenesis.

Keywords:

PRF, Platelet-Rich Fibrin, Osteogenesis

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Kiernan C, Knuth C, Farrell E. Endochondral Ossification: Recapitulating Bone Development for Bone Defect Repair. Amsterdam, Netherlands: Elsevier Inc.; 2018. DOI: https://doi.org/10.1016/B978-0-12-811467-4.00006-1

Agrawal M, Agrawal V. Platelet rich fibrin and its applications in dentistry-a review article. Natl J Med Dent Res. 2014;2(3):51-8.

Calori GM, Mazza E, Colombo M, Ripamonti C. The use of bone-graft substitutes in large bone defects: Any specific needs? Injury. 2011;42(2):S56-63. https://doi.org/10.1016/j.injury.2011.06.011 PMid:21752369 DOI: https://doi.org/10.1016/j.injury.2011.06.011

Kanakamedala A, Ari G, Sudhakar U, Vijayalakshmi R, Ramakrishnan T, Emmadi P. Treatment of a furcation defect with a combination of platelet-rich fibrin (PRF) and bone graft-a case report. ENDO. 2009;3(2):127-35.

Najeeb S, Khurshid Z, Agwan MA, Ansari SA, Zafar MS, Matinlinna JP. Regenerative potential of platelet rich fibrin (PRF) for curing intrabony periodontal defects: A systematic review of clinical studies. Tissue Eng Regen Med. 2017;14(6):735-42. https://doi.org/10.1007/s13770-017-0079-5 PMid:30603523 DOI: https://doi.org/10.1007/s13770-017-0079-5

Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gøtzsche PC, Ioannidis JP, et al. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate healthcare interventions: Explanation and elaboration. BMJ. 2009;339:b2700. https://doi.org/10.1136/bmj.b2700 PMid:19622552 DOI: https://doi.org/10.1136/bmj.b2700

Sumida R, Maeda T, Kawahara I, Yusa J, Kato Y. Platele-trich fibrin increases the osteoprotegerin/receptor activator of nuclear factorκB ligand ratio in osteoblasts. Exp Ther Med. 2019;18(1):358-65. https://doi.org/10.3892/etm.2019.7560 PMid:31258673 DOI: https://doi.org/10.3892/etm.2019.7560

Dohle E, El Bagdadi K, Sader R, Choukroun J, Kirkpatrick CJ, Ghanaati S. Platelet-rich fibrin-based matrices to improve angiogenesis in an in vitro co-culture model for bone tissue engineering. J Tissue Eng Regen Med. 2018;12(3):598-610. https://doi.org/10.1002/term.2475 PMid:28509340 DOI: https://doi.org/10.1002/term.2475

Kargarpour Z, Nasirzade J, Strauss FJ, Di Summa F, Hasannia S, Müller HD, et al. Platelet-rich fibrin suppresses in vitro osteoclastogenesis. J Periodontol. 2020;91(3):413-21. https://doi.org/10.1002/JPER.19-0109 PMid:31381154 DOI: https://doi.org/10.1002/JPER.19-0109

Zhang K, Barragan-Adjemian C, Ye L, Kotha S, Dallas M, Lu Y, et al. E11/gp38 selective expression in osteocytes: Regulation by mechanical strain and role in dendrite elongation. Mol Cell Biol. 2006;26(12):4539-52. https://doi.org/10.1128/MCB.02120-05 PMid:16738320 DOI: https://doi.org/10.1128/MCB.02120-05

Wong PC, Wang CY, Jang JS, Lee CH, Wu JL. Large-pore platelet-rich fibrin with a mg ring to allow mc3t3-e1 preosteoblast migration and to improve osteogenic ability for bone defect repair. Int J Mol Sci. 2021;22(8):4022. https://doi.org/10.3390/ijms22084022 PMid:33919677 DOI: https://doi.org/10.3390/ijms22084022

Thanasrisuebwong P, Kiattavorncharoen S, Surarit R, Phruksaniyom C, Ruangsawasdi N. Red and yellow injectable platelet-rich fibrin demonstrated differential effects on periodontal ligament stem cell proliferation, migration, and osteogenic differentiation. Int J Mol Sci. 2020;21(14):5153. https://doi.org/10.3390/ijms21145153 PMid:32708242 DOI: https://doi.org/10.3390/ijms21145153

Wong CC, Liao JH, Sheu SY, Lin PY, Chen CH, Kuo TF. Novel transplant of combined platelet-rich fibrin Releasate and bone marrow stem cells prevent bone loss in Ovariectomized osteoporotic mice. BMC Musculoskelet Disord. 2020;21(1):527. https://doi.org/10.1186/s12891-020-03549-y PMid:32770974 DOI: https://doi.org/10.1186/s12891-020-03549-y

Ghaznavi D, Babaloo A, Shirmohammadi A, Zamani AR, Azizi M, Rahbarghazi R, et al. Advanced platelet-rich fibrin plus gold nanoparticles enhanced the osteogenic capacity of human mesenchymal stem cells. BMC Res Notes. 2019;12(1):721. https://doi.org/10.1186/s13104-019-4750-x PMid:3168501 DOI: https://doi.org/10.1186/s13104-019-4750-x

Yilmaz D, Dogan N, Ozkan A, Sencimen M, Ora BE, Mutlu I. Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs. Acta Cir Bras. 2014;29(1):59-65. https://doi.org/10.1590/S0102-86502014000100009 PMid:24474179 DOI: https://doi.org/10.1590/S0102-86502014000100009

Golub E, Battaglia K. The role of alkaline phosphatase in osteogenesis. J Exp Med. 1951;93(5):415-26. https://doi.org/10.1084/jem.93.5.415 DOI: https://doi.org/10.1084/jem.93.5.415

Kawane T, Qin X, Jiang Q, Miyazaki T, Komori H, Yoshida CA, et al. Runx2 is required for the proliferation of osteoblast progenitors and induces proliferation by regulating Fgfr2 and Fgfr3. Sci Rep. 2018;8(1):13551. https://doi.org/10.1038/s41598-018-31853-0 PMid:30202094 DOI: https://doi.org/10.1038/s41598-018-31853-0

Zhang Y, Gong H, Sun Y, Huang Y, Fan Y. Enhanced osteogenic differentiation of MC3T3-E1 cells on grid-topographic surface and evidence for involvement of YAP mediator. J Biomed Mater Res A. 2016;104(5):1143-52. https://doi.org/10.1002/jbm.a.35648 PMid:26748630 DOI: https://doi.org/10.1002/jbm.a.35648

Gradin P, Hollberg K, Cassady AI, Lång P, Andersson G. Transgenic overexpression of tartrate-resistant acid phosphatase is associated with induction of osteoblast gene expression and increased cortical bone mineral content and density. Cells Tissues Organs. 2012;196(1):68-81. https://doi.org/10.1159/000330806 PMid:22248481 DOI: https://doi.org/10.1159/000330806