Study of Lipid Peroxidation-antioxidant Defense Systems in Rats under Radiation Exposure
DOI:
https://doi.org/10.3889/oamjms.2022.8352Keywords:
Radiation, Lipid peroxidation products, Catalase, Antioxidant systemAbstract
Abstract
BACKGROUND: Experimental data on the effect of a high dose of gamma radiation 6 Gy on the activity of antioxidant defense enzymes and lipid peroxidation products (LPO) are presented immunocompetent organs in the body of animals in the experiment. It was found that the effect of ionizing radiation led to an increase in the level of diene conjugates (DC) and malondialdehyde (MDA), inhibition of the activity of catalase (CАT) and glutathione peroxidase (GlP), glutathione reductase (GlR) enzymes in almost all the studied objects, as a result of which the development of oxidative stress was observed in them. The results of the study indicate serious changes in the lipid peroxidation and antioxidant system (AOS) under radiation stress. An imbalance of POL-AOS creates prerequisites for the occurrence of immunopathological conditions, contributing to the development of radiation-related tumor infection. pathologies. Violations of functional relationships of the catalytic redox system.
AIM: The aim of the study was to study the effect of radiation at a dose of 6 Gy on the parameters of lipoperoxidation and the antioxidant system in experimental rats.
MATERIAL AND METHODS: The work was carried out on 20 male Wistar rats weighing 240 ± 20 g. Experimental animals are divided into two groups: I - normal group; II - group exposed to γ-rays at a dose of 6 Gy.
RESULTS: After radiation exposure, all objects showed a tendency to increase the product of lipid peroxidation. As is known, the activation of lipid peroxidation is based on excessive generation of reactive oxygen species, which exceeds the physiological capabilities of antioxidant systems that occur after the depletion of enzyme systems.
CONCLUSION: Based on the results obtained, it was found, that irradiation increases the intensity of the formation of POL products and their accumulation, reduces the activity of enzymes of the antioxidant system in immunocompetent organs in irradiated animals, as a result, the lack of the antioxidant system causes oxidative stress in the body.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Yarmonenko SP. Chernobl we look back to go forward. In: Medical Radiology and Radiation Safety. Vol. 5. Atomizdat Publishing House, Moscow; 2005. p. 77-80.
Chulenbayeva L, Ilderbayev O, Taldykbayev Z, Ilderbayeva G, Argynbekova A. Phytocorrection of immunological and biochemical changes in the combined impact of coal dust and high dose of radiation. Georgian Med News. 2018;2(275):141-50. PMid:29578440
Wilson PF. Magnification of inter-individual variation in biological responses after low doses and dose-rates of ionizing radiation. Health Phys. 2016;110(3):296-8. https://doi.org/10.1097/HP.0000000000000453 PMid:26808888 DOI: https://doi.org/10.1097/HP.0000000000000453
Tapbergenov SO, Zhetpisbaev BA, Ilderbayev OZ, Usenova OA, Ilderbayeva GO. Free radical oxidation in rats in the delayed period after combined exposure to dust and radiation. Bull Exp Biol Med. 2013;154(6):747-9. https://doi.org/10.1007/s10517-013-2046-z PMid:23658914 DOI: https://doi.org/10.1007/s10517-013-2046-z
Ilderbayeva G, Zhetpisbaev B, Ilderbayev O, Taldykbayev Z, Bekeeva S. Metabolic processes of organism in remote period after the combined effects of radiation and emotional stress. Georgian Med News. 2016;250:76-82. PMid:26870980
Datta K, Suman S, Trani D, Doiron K, Rotolo JA, Kallakury BV, et al. Accelerated hematopoietic toxicity by high-energy (56) Fe radiation. Int J Radiat Biol. 2012;88(3):213-22. https://doi.org/10.3109/09553002.2012.639434 PMid:22077279 DOI: https://doi.org/10.3109/09553002.2012.639434
Ivanov VK, Evstratov EV, Chekin SY, Kazakov SV, Menyailo AN. Optimization of radiation protection of personnel taking into account the limitations of the generalized risk of potential exposure. Radiat Risk. 2010;19(3):59-70.
Reisz JA, Bansal N, Qian J, Zhao W, Furdui CM. Effects of ionizing radiation on biological molecules--mechanisms of damage and emerging methods of detection. Antioxid Redox Signal. 2014;21(2):260-92. https://doi.org/10.1089/ars.2013.5489 PMid:24382094 DOI: https://doi.org/10.1089/ars.2013.5489
Little DB. Unmarked effects of ionizing radiation: Conclusions applied to low-dose effects. Radiat Biol Radioecol. 2007;47(3):262-72.
Tom KH. Response of biological systems to low doses of ionizing radiation. Health Phys. 2016;110(3):281-2. https://doi.org/10.1097/HP.0000000000000452 PMid:26808883 DOI: https://doi.org/10.1097/HP.0000000000000452
Zentov NK. Oxidative stress. In: Zentov NK, Lankin VZ, Menshchikova EB, editors. Biochemical and Pathophysiological Aspects. Moscow: Science; 2001. p. 340.
Lukyanova LD. Modern problems of adaptation to hypoxia. Signaling mechanisms and their role in systemic regulation. Patol Physiol Exp Ther. 2011;1:3-19.
Abdikadirova HR, Amreeva KE, Kalishev MG, Zhautikova SB. Evaluation of the ef ectiveness of alimentary correction of pathological changes in hepatic tissue under the inf uence of industrial copper-containing dust in the experiment. Russ J Occup Health Ind Ecol. 2019;1(7):438-43. https://doi.org/10.31089/1026-9428-2019-59-7-438-443 DOI: https://doi.org/10.31089/1026-9428-2019-59-7-438-443
Surinov BP, Karpova NA. Combined effect of ionizing radiation and stress on antibody formation in mice. Radiat Biol Radioecol. 1996;36(3):359-64. PMid:3628739
Melnik SN, Naumov AD, Barry LG. Influence of stress and radiation effects on biochemical parameters of rat blood serum. Health Ecol Probl. 2007;2: 132-37.
Madieva MR, Musainova AK, Zhetpisbayev BA, Saidakhmetova AS, Zhetpisbayeva KS. Adaptation of the Humoral Link of Immunity, in the Long-term Period, after Exposure to Fractionated Gamma Radiation; 2014;2:25-7.
Abdikadirova KR, Amreyeva KT, Zhautikova SB, Kostyleva OA, Abikenova FS, Chergizova BT, et al. Morphological changes in the hepatic tissue at the impact of industrial copper-bearing dust in the experiment. Open Access Maced J Med Sci. 2020;8(E):1-4. https://doi.org/10.3889/oamjms.2020.3473 DOI: https://doi.org/10.3889/oamjms.2020.3473
Abikenova F, Meyramov G, Zhautikova S, Abdikadirova H, Zhienbayeva C, Talaspekova Y, et al. Investigation of antidiabetogenic effect of the iodine-selenium concentrate in animals with chronic alloxan diabetes of varying severity. Open Access Maced J Med Sci. 2021;9(A):535-40. https://doi.org/10.3889/oamjms.2021.5873 DOI: https://doi.org/10.3889/oamjms.2021.5873
Okassova AK, Ilderbayev OZ, Nursafina AZ, Zharmakhanova GM, Rakhimova BB, Yessilbaeva BT, et al. Evaluation of lipid peroxidation under immobilization stress in irradiated animals in experiment. Open Access Maced J Med Sci. 2021;9(A): 119-22. https://doi.org/10.3889/oamjms.2021.5781. DOI: https://doi.org/10.3889/oamjms.2021.5781
Korolyuk MA, Ivanova LI, Mayorova NO, Tokarev VE. Method for determining catalase activity. Lab Delo. 1988;1:16-9. PMid:2451064
Koichubekov BK. Biostatistics: A Training Manual. Almaty: Evero; 2015. p. 152.
Downloads
Published
How to Cite
License
Copyright (c) 2022 Assem K. Okassova, Valeriy Britko, Didar B. Okassov, Yelena S. Tatina, Akerke I. Tolegenova, Kuttykyz N. Kuvatbaeva, Gulzhan T. Kaliyeva, Madina Zhunussova, Nazira Duzbaeva (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
