DPP-IV Inhibitory Activity of the Ethanolic Extract of Red Gedi Leaves Abelmoschus manihot L. Medic


  • Juliet Tangka Doctoral Program of Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Pharmacy, Manado Health Polytechnic, Ministry of Health, Manado, Indonesia
  • Elisabeth Natalia Barung Department of Pharmacy, Manado Health Polytechnic, Ministry of Health, Manado, Indonesia
  • Diana Lyrawati Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia https://orcid.org/0000-0001-5931-3245
  • Djoko Soeatmadji Department of Internal Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Nurdiana Nurdiana Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia




Abelmoschus manihot L. Medic, DPP-IV inhibitor, Molecular docking, In vitro


BACKGROUND: At present, there are many drugs used to manage diabetes including dipeptidyl peptidase-4 (DPP-IV) inhibitors which target insulin secretion. Abelmoschus manihot L. Medic, an endemic species of Minahasa, Indonesia, has been used as an antidiabetic herbal medicine.

AIM: In this study, we studied its metabolites activities, in silico and in vitro, as inhibitor for DPP-IV, thus regulating insulin secretion.

RESULTS: Of 38 identified metabolites, when docked into the catalytic site DPP-IV, 10 showed good binding energy within range of the standard gliptin drugs, that is, hibiscetin, gossypentin, gossypetin - 3-glucoside, myricetin, myricetin 3-glucoside, alpha spinasterol, quercetin, syringaresinol, stigmasterol, and isoquercetin. Three of those ten metabolites showed Ki within standard drugs values, that is, gossypetin, alpha spinasterol, and stigmasterol. The profile of molecular dynamic simulation, total energy and root mean square deviation of those metabolites were all similar with the standard gliptin drugs and predicted good stability of the complexes. The subsequent in vitro assay determining DPP-IV activity of the red Gedi leaves extract demonstrated that indeed the extract inhibited DPP-IV activity with IC50 860.67 μg/mL. Further studies are ongoing to prove the antidiabetic properties of the whole as well as isolated single compounds of the extract in particular gossypetin, alpha spinasterol, and stigmasterol as DPP-IV inhibitors.

CONCLUSION: Our in silico studies showed that the compounds of ethanolic extract of red Gedi leaves potentially serve as DPP-IV inhibitors. Based on computed binding affinity, Ki, total energy, RMSD, and stability, the most potent compounds of the extract to inhibit DPP-IV activity are probably gossypetin, alpha spinasterol, and stigmasterol.


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How to Cite

Tangka J, Barung EN, Lyrawati D, Soeatmadji D, Nurdiana N. DPP-IV Inhibitory Activity of the Ethanolic Extract of Red Gedi Leaves Abelmoschus manihot L. Medic. Open Access Maced J Med Sci [Internet]. 2022 Jan. 26 [cited 2023 Mar. 22];10(A):207-13. Available from: https://oamjms.eu/index.php/mjms/article/view/8356

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