Antioxidant Activity of Laportea decumana (Roxb) Wedd Ethanol and n-Hexane Extracts

Authors

  • Abdul Thalib Doctoral Student, Program of Medicine, Medical Faculty, Hasanuddin University, Makassar, Indonesia; Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia https://orcid.org/0000-0002-4009-6284
  • Rina Masadah Department of Pathology Anatomy, Hasanuddin University, Makassar, Indonesia
  • Prihartono Prihartono Department of Surgical Science, Hasanuddin University, Makassar, Indonesia
  • Firdaus Hamid Department of Microbiology, Hasanuddin University, Makassar, Indonesia
  • Muhammad Haidir Department of Pharmacy, Makassar Islamic University, Makassar, Indonesia
  • Hulan Hasan Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia
  • Sudin Keliwawa Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia
  • Irman Labulawa Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8399

Keywords:

Laportea decumana (Robx) wedd, Antioxidant, Phytochemical test

Abstract

Background: Laportea decumana (robx.) wedd. is a traditional medicine used by Indonesian people, especially in eastern Indonesia, Maluku Ambon. But its utilization has not been based on evidence-based research, so it still needs to be developed to be used as complementary nursing therapy in the future.

Aim: This study aims to determine the phytochemical content and antioxidant activity of Laportea decumana (robx.) wedd. from Ambon Maluku.

Method: Extraction is done by the maceration method with 96% ethanol solvent. Phytochemical tests are performed with thin-layer chromatography (TLC) to look at flavonoids, tannins and triterpenoids, then alkaloids and saponins using tube tests. The antioxidant activity test was conducted using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method.

Results: Laportea decumana (robx.) wedd. phytochemical test positively contains alkaloids, flavonoids, tannins, triterpenoids and saponins. The antioxidant activity of Laportea Decumana (Robx) Wedd Ethanol Extract (LDrW-EE) 22.81 ppm, Laportea Decumana (Robx) Wedd n-Hexane Extract (LDrW-NHE) 44.89 ppm and vitamin C 6.03 ppm.

Conclusion: LDrW-EE antioxidant activity is better when compared to LDrW-NHE but no better than vitamin C

 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Kusmana C, Hikmat A. Keanekaragaman hayati flora di Indonesia. J Pengelolaan Sumberd. 2015;5(2):187-98. https://doi.org/10.19081/jpsl.5.2.187 DOI: https://doi.org/10.19081/jpsl.5.2.187

Lindquist R, Snyder M, Tracy MF. Complementary and Alternative Therapies in Nursing. 8th ed. United States of America: Springer Publishing Company; 2018. DOI: https://doi.org/10.1891/9780826144348

Thalib A, Masadah R, Prihartono P, Hamid F, Hasan H, Keliwawa S, et al. Laportea decumana (Robx) Wedd. Herbal endemic potential from Indonesia: A literature review. Maced J Med Sci. 2021;9:639-43. https://doi.org/10.3889/oamjms.2021.7759 DOI: https://doi.org/10.3889/oamjms.2021.7759

Yadav A, Kumari R, Yadav A, Mishra JP, Seweta S, Prabha S. Antioxidants and its functions in human body-a review. Res Environ Life Sci. 2016;9(11):1328-31.

Salehi B, Martorell M, Arbiser JL, Sureda A, Martins N, Maurya PK, et al. Antioxidants: Positive or negative actors? Biomolecules. 2018;8(4):124. https://doi.org/10.3390/biom8040124 PMid:30366441 DOI: https://doi.org/10.3390/biom8040124

Ozyurt D, Demirata B, Apak R. Determination of total antioxidant capacity by a new spectrofluorometric method based on Ce(IV) reduction: Ce(III) fluorescence probe for CERAC assay. J Fluoresc. 2011;21(6):2069-76. https://doi.org/10.1007/s10895-011-0905-4 PMid:21617997 DOI: https://doi.org/10.1007/s10895-011-0905-4

Astuti E, Sunarminingsih R, Jenie UA, Mubarika S, Sismindari S. Pengaruh lokasi tumbuh, umur tanaman dan variasi jenis destilasi terhadap komposisi senyawa minyak atsiri rimpang curcuma mangga produksi beberapa sentra di Yogyakarta. J Mns dan Tumbuh. 2014;21(3):2-7.

Simaremare ES, Holle E, Budi IM, Yabansabra Y. Analisis perbandingan efektivitas antinyeri salep daun gatal dari simplisia Laportea decumana dan Laportea sp. J Farm Indones. 2015;12(1):1-10.

Cepeda GN, Murtiningrum, Orisore DL. Kapasitas donasi atom hidrogen dan kelasi metal ektrak daun gatal (Laportea decumana (Roxb.) Wedd.). J Sains dan Teknol Pangan. 2021;6(1):3603-12.

Hestiningtyas B, Siallagan J, Holle E. Uji aktivitas ekstrak daun gatal (Laportea decumanum (Roxb.) kuntze) sebagai antioksidan. AVOGARDRO. 2019;3(1):1-5.

Dharma MA, Nocianitri KA, Yusasrini NL. Pengaruh metode pengeringan simplisia terhadap kapasitas antioksidan Wedang Uwuh. J Ilmu Teknol Pangan. 2020;9(1):88. https://doi.org/10.24843/itepa.2020.v09.i01.p11 DOI: https://doi.org/10.24843/itepa.2020.v09.i01.p11

Thanh TB, Duc LV, Thanh HN, Tien VN. In vitro antioxidant and anti-inflammatory activities of isolated compounds of ethanol extract from Sanchezia speciosa Leonard’s leaves. J Basic Clin Physiol Pharmacol. 2016;28(1):79-84. https://doi.org/10.1515/ jbcpp-2016-0086 PMid:27861139 DOI: https://doi.org/10.1515/jbcpp-2016-0086

Noerhartati E, Rizal MF, Joeniarti E. Phytochemical screening of sorghum (Sorghum Sp) with thin layer chromatography method. Spirit Soc J. 2020;3(2):8-12. https://doi.org/10.29138/scj.v3i2.1087 DOI: https://doi.org/10.29138/scj.v3i2.1087

Chaves N, Santiago A, Alías JC. Quantification of the antioxidant activity of plant extracts: Analysis of sensitivity and hierarchization based on the method used. Antioxidants. 2020;9(1):76. https://doi.org/10.3390/antiox9010076 PMid:31952329 DOI: https://doi.org/10.3390/antiox9010076

Lo SF, Mulabagal V, Chen CL, Kuo CL, Tsay HS. Bioguided fractionation and isolation of free radical scavenging components from in vitro propagated chinese medicinal plants Dendrobium tosaense Makino and Dendrobium moniliforme SW. J. Agric Food Chem. 2004;52(23):6916-9. https://doi.org/10.1021/jf040017r PMid:15537296 DOI: https://doi.org/10.1021/jf040017r

Sukandar D, Nurbayti S, Rudiana T, Husna TW. Isolation and structure determination of antioxidants active compounds from ethyl acetate extract of heartwood namnam (Cynometra cauliflora L.). J Kim Terap Indones. 2017;19(1):11-7. https://doi.org/10.14203/jkti.v19i1.325 DOI: https://doi.org/10.14203/jkti.v19i1.325

Rivero-Cruz JF, Granados-Pineda J, Pedraza-Chaverri J, Pérez-Rojas JM, Kumar-Passari A, Diaz-Ruiz G, et al. Phytochemical constituents, antioxidant, cytotoxic, and antimicrobial activities of the ethanolic extract of mexican brown propolis. Antioxidants. 2020;9(1):70. https://doi.org/10.3390/antiox9010070 PMid:31940981 DOI: https://doi.org/10.3390/antiox9010070

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Int Pharm Sci. 2011;1:98-106.

Simaremare ES. Skrining fitokimia ekstrak etanol daun gatal (Laportea decumana (Roxb.) Wedd). Pharmacy. 2014;11(1):98-107.

Ariastuti R, Anam K, Pamungkas IY. Efektifitas analgesik daun gatal (Laportea decumana) pada penderita myalgia di kampung ATSJ Distrik ATSJ kabupaten asmat provinsi papua. J Kebidanan. 2018;10(1):50-61. DOI: https://doi.org/10.35872/jurkeb.v10i01.298

Simaremare ES, Gunawan E, Yarangga I, Satya MD, Yabansabra YR. Antibacterial and toxicity activities itchy leaves (Laportea decumana, Roxb. Wedd) extract. J Phys Conf Ser. 2020;1503(1):012041. https://doi.org/10.1088/1742-6596/1503/1/012041 DOI: https://doi.org/10.1088/1742-6596/1503/1/012041

Moure A, Cruz JM, Franco D, Domı́ nguez JM, Sineiro J, Domı́ nguez H, et al. Natural antioxidants from residual sources. Food Chem. 2001;72(2):145-71. https://doi.org/10.1016/S0308-8146(00)00223-5 DOI: https://doi.org/10.1016/S0308-8146(00)00223-5

El Houda Lezoul N, Belkadi M, Habibi F, Guillén F. Extraction processes with several solvents on total bioactive compounds in different organs of three medicinal plants. Molecules. 2020;25(20):4672. https://doi.org/10.3390/molecules25204672 PMid:33066273 DOI: https://doi.org/10.3390/molecules25204672

Haerani A, Chaerunisa AY, Subarnas A. Antioxidant activities of Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa using DPPH Method. Indones J Pharm. 2019;1(2):57-61. https://doi.org/10.24198/idjp.v1i2.21531 DOI: https://doi.org/10.24198/idjp.v1i2.21531

Chicco D, Warrens MJ, Jurman G. The coefficient of determination R-squared is more informative than SMAPE, MAE, MAPE, MSE and RMSE in regression analysis evaluation. PeerJ Comput Sci. 2021;7:e623. https://doi.org/10.7717/PEERJ-CS.623 PMid:34307865 DOI: https://doi.org/10.7717/peerj-cs.623

Downloads

Published

2022-03-14

How to Cite

1.
Thalib A, Masadah R, Prihartono P, Hamid F, Haidir M, Hasan H, Keliwawa S, Labulawa I. Antioxidant Activity of Laportea decumana (Roxb) Wedd Ethanol and n-Hexane Extracts. Open Access Maced J Med Sci [Internet]. 2022 Mar. 14 [cited 2024 Mar. 28];10(A):590-4. Available from: https://oamjms.eu/index.php/mjms/article/view/8399

Most read articles by the same author(s)