Antioxidant Activity of Laportea decumana (Roxb) Wedd Ethanol and n-Hexane Extracts

Abdul Thalib; Rina Masadah; Prihartono Prihartono; Firdaus Hamid; Muhammad Haidir; Hulan Hasan; Sudin Keliwawa; Irman Labulawa

doi:10.3889/oamjms.2022.8399

Authors

Abdul Thalib Doctoral Student, Program of Medicine, Medical Faculty, Hasanuddin University, Makassar, Indonesia; Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia https://orcid.org/0000-0002-4009-6284
Rina Masadah Department of Pathology Anatomy, Hasanuddin University, Makassar, Indonesia
Prihartono Prihartono Department of Surgical Science, Hasanuddin University, Makassar, Indonesia
Firdaus Hamid Department of Microbiology, Hasanuddin University, Makassar, Indonesia
Muhammad Haidir Department of Pharmacy, Makassar Islamic University, Makassar, Indonesia
Hulan Hasan Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia
Sudin Keliwawa Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia
Irman Labulawa Department of Nursing, High School of Health Science Pasapua Ambon, Ambon, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8399

Keywords:

Laportea decumana (Robx) wedd, Antioxidant, Phytochemical test

Abstract

Background: Laportea decumana (robx.) wedd. is a traditional medicine used by Indonesian people, especially in eastern Indonesia, Maluku Ambon. But its utilization has not been based on evidence-based research, so it still needs to be developed to be used as complementary nursing therapy in the future.

Aim: This study aims to determine the phytochemical content and antioxidant activity of Laportea decumana (robx.) wedd. from Ambon Maluku.

Method: Extraction is done by the maceration method with 96% ethanol solvent. Phytochemical tests are performed with thin-layer chromatography (TLC) to look at flavonoids, tannins and triterpenoids, then alkaloids and saponins using tube tests. The antioxidant activity test was conducted using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) method.

Results: Laportea decumana (robx.) wedd. phytochemical test positively contains alkaloids, flavonoids, tannins, triterpenoids and saponins. The antioxidant activity of Laportea Decumana (Robx) Wedd Ethanol Extract (LDrW-EE) 22.81 ppm, Laportea Decumana (Robx) Wedd n-Hexane Extract (LDrW-NHE) 44.89 ppm and vitamin C 6.03 ppm.

Conclusion: LDrW-EE antioxidant activity is better when compared to LDrW-NHE but no better than vitamin C

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