Antidiabetic Effect Test of Insulin Stem Extract (Tithonia diversifolia) Toward Streptozotocin-Induced Diabetic Rats (Rattus Norvegicus)

Suherman Suherman; Baharuddin Hamzah; Sri Hastuti V. Pulukadang; Sitti Rahmawati; Muhammad Fakhrul Hardani; Ririen Hardani; Andi Saifah

doi:10.3889/oamjms.2022.8411

Authors

Suherman Suherman Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Baharuddin Hamzah Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Sri Hastuti V. Pulukadang Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Sitti Rahmawati Chemistry Study Program, Mathematics and Sciences Division, Faculty of Teacher Training and Education, Tadulako University, Palu, Indonesia
Muhammad Fakhrul Hardani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Indonesia
Ririen Hardani Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu, Indonesia
Andi Saifah Nursing Study Program, Faculty of Medicine, Tadulako University, Palu, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8411

Keywords:

Antidiabetic, Insulin stem extract, Tithonia diversifolia, Streptozotocin

Abstract

Insulin Plant is a plant that has potency to be used as an antidiabetic drug. This is due to the presence of the chemical content of alkaloids, flavonoids, saponins, tannins and polyphenols. A research on the effects of insulin antidiabetic extract stem test (Tithonia diversifolia [Hemsl] A. Gray) in the which stem insulin was extracted by maceration using 96% ethanol, antidiabetic tests conducted using test animals mice induced with streptozotocin 40 mg/kg intraperitoneally. Tested animals were Divided into 5 groups, call now was given a different treatment. The treatment I was given a suspension of 1% Na CMC as a negative control. Treatment II, III and IV administered insulin stem extract per call now with a dose of 125 mg / kg, 250 mg / kg and 500 mg / kg. Treatment of V given glibenclamide suspension as a positive control. Blood glucose levels were measured using glucometer mice. Data were Analyzed using ANOVA statistical tests at 95% confidence level. The analysis shows that the stem extract of insulin (Tithonia diversifolia) is proven to lower blood glucose levels Significantly for rats, after treatment for 14 days and the dose of insulin stem extract are effective in lowering the blood glucose level is 250 mg / kg.

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