Vascular Endothelial Growth Factor, Epidermal Growth Factor, and Epithelialization Analysis on Full-Thickness Wound Applied with Topical Erythropoietin

Noi Maya Anggrita Sari; Iswinarno Doso Saputro; Magda Rosalina Hutagalung

doi:10.3889/oamjms.2022.8476

Authors

Noi Maya Anggrita Sari Department of Plastic Reconstructive Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia https://orcid.org/0000-0002-7463-137X
Iswinarno Doso Saputro Department of Plastic Reconstructive Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Magda Rosalina Hutagalung Department of Plastic Reconstructive Aesthetic Surgery, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.8476

Keywords:

Epidermal growth factor, Epithelialization rate, Medical care, Vascular endothelial growth factor, Wound healing

Abstract

BACKGROUND: Skin wounds are a major challenge, such as full-thickness wounds that need a long time to heal. However, the addition of erythropoietin can accelerate the wound healing process.

AIM: This study was aimed to determine the effect of topical erythropoietin administration on vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and epithelialization in the wound healing process.

METHODS: This was randomized controlled trial. Full-thickness wounds were created on the back of each white rat. No treatment was administered in the control group (n=16), and topical erythropoietin was applied in the treatment group (n=16). The rats were euthanized on day 3 and day 6 post-surgery, respectively. The expression levels of VEGF, EGF, and microscopic epithelialization rate were examined.

RESULTS: The level of EGF expression in the treatment group increased significantly on day 3 by 2.84 times compared to the control group and on day 6 increased to 4.89 times compared to the control group (p < 0.001). The level of VEGF expression in the treatment group on day 3 increased 2.18 times compared to the control group and on day 6 increased to 2.65 times compared to the control group (p < 0.001). Meanwhile, epithelialization in the treatment group on day 3 increased 1.3 times compared to the control group and on day 6 increased up to 7.62 times compared to the control group (p < 0.001).

CONLUSION: Topical administration of erythropoietin could increase the expression of VEGF, EGF, and epithelialization in both early inflammatory and proliferative phases.

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