Effect of Standardised Extract Pegagan Embun (Hydrocotyle sibthorpioides Lam.) toward Natural Killer Cell and CD8 Cell Activities on White Male Mice Exposed to H5N1 Virus Antigen

Afriwardi Afriwardi; Fitratul Wahyuni; Elidahanum Husni; Aditya Alqamal Alianta; Yufri Aldi

doi:10.3889/oamjms.2022.8667

Authors

Afriwardi Afriwardi Department of Physiology, Faculty of Medicine, Universitas Andalas, Padang, West Sumatera, Indonesia
Fitratul Wahyuni Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera, Indonesia
Elidahanum Husni Department of Biology of Pharmacy, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera, Indonesia
Aditya Alqamal Alianta Department of Socio-economics, Faculty of Animal Science, Universitas Andalas, Padang, West Sumatera, Indonesia https://orcid.org/0000-0002-0460-5862
Yufri Aldi Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Padang, West Sumatera, Indonesia https://orcid.org/0000-0002-4822-4543

DOI:

https://doi.org/10.3889/oamjms.2022.8667

Keywords:

Hydrocotyle sibthorpioides Lam., Immunostimulan, Natural killer cell, CD8 cell

Abstract

AIM: Community in Indonesia used pegagan embun (Hydrocotyle sibthorpioides Lam.) to increase endurance. Based on that, the study aimed to determine the effects of pegagan embun extract on natural killer (NK) cells and CD8 cells activities in white male mice exposed to the H5N1 antigen.

MATERIALS AND METHODS: As many as thirty-five experimental white male mice were used, divided into seven groups, by varying the time of administration of the H5N1 antigen. Groups I and II, were given the test extract for 7 days and induced H5N1 antigen on days 1 and 7, then evaluated on day 8. Group III was given a test extract for 3 days, then induced antigen on the 4th day and continued with giving the test extract until day 7th. In Groups V and VII, only H5N1 antigen induction on day 1 and evaluated on day 8. Groups IV and VI were given the test extract for 7 and 4 days. The observations examine the activities of NK cells and CD8 cells. The data were analyzed with a one-way analysis of variation method with p = 0.05, then analyzed with Duncan Multiple Range Test.

RESULTS AND DISCUSSION: After calculating, the NK cell activity for groups 1 to 7, respectively: 2.12; 2.03; 2.07; 1.87; 1.98; 1.91; and 1.95 ng/mL while for CD8 cell activity 22.23; 24.61; 23.69; 21.10; 19,20; 19.87; and 18.13 ng/mL. The results showed that giving pegagan embun extract to white male mice exposed to the H5N1 antigen increase the activities of NK cells and CD8 cells.

CONCLUSION: It concluded that administration of standardized extract of pegagan embun (H. sibthorpioides Lam.) at a dose of 200 mg/kg BW increased NK cells activities and CD8 cells of white male mice exposed to H5N1 virus antigen.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Marbun RA, Situmorang N, Wahyuni S. The effect of immunomodulator by extract ethanol of herba binary (Artemisia vulgaris L.) Toward the response of delayed-type hypersensitivity in rat males. J Penelitian Farm Herb. 2018;1(1):17-21.

Hasdianah, Dewi P, Peristiowati, Imam SS. Imunologi. Diagnosis dan Teknik Biologi Molekuler. Yogyakarta: Nuha Medika; 2014.

Radji M. Imunologi and Virologi. Jakarta Barat: PT ISFI Penerbitan; 2015.

Lestari YE. Analisis Model Matematika Pada Interaksi Sistem Imun Dengan Pertumbuhan sel Tumor (Doctoral Dissertation, Universitas Islam Negeri Maulana Malik Ibrahim); 2011.

Supatmo Y, Susanto H, Sugiharto S. The Effect of exercise on NK Cell as Immune Indicators. Med Keolahragaan Indones. 2015;5(2):30-6.

Meiliana N. The Effect of Ethanol Extract of Eleuthrine palmifolia L. Merr orally administration on BALB/c Mice or to mitigate of NK Cel and CD 8+ Decreasing. J Bios Pascasarjana. 2016;18(1):13-23. DOI: https://doi.org/10.20473/jbp.v18i1.2016.13-23

Kresno S. Imunologi: Diagnosis dan Prosedur Laboratorium. 5th ed. Jakarta: FKUI Press; 2010.

Singh Y, Fuloria NK, Fuloria S, Subramaniyan V, Almalki WH, Gupta G, et al. Disruption of the biological activity of protease-activated receptors2/4 in adults rather than children in SARS CoV-2 virus-mediated mortality in COVID-19 infection. Drug Dev Res. 2021;82(8):1075-8. https://doi.org/10.1002/ddr.21874 PMid:34469011 DOI: https://doi.org/10.1002/ddr.21874

Singh Y, Fuloria NK, Fuloria S, Subramaniyan V, Meenakshi DU, Chakravarthi S, et al. N-terminal domain of SARS CoV-2 spike protein mutation associated reduction in effectivity of neutralizing antibody with vaccinated individuals. J Med Virol. 2021;93(10):5726-8. https://doi.org/10.1002/jmv.27181 PMid:34232521 DOI: https://doi.org/10.1002/jmv.27181

Kusnul Z. COVID-19 Infection and Immune-system: The role of herbal medicine based on efficacious natural products. J Ilmiah Pamenang. 2020;2(2):26-31. DOI: https://doi.org/10.53599/jip.v2i2.72

Nugroho YA. The Effect of Piper betle L. fruits Plectranthus scutellariodes L. leafs honey, and yolk combination administrated on activities improvement of phagocytic capacity of macrophages. Med Litbang Kesehatan. 2012;22(1):1-5.

Subramaniyan V, Chakravarthi S, Seng WY, Kayarohanam S, Fuloria NK, Fuloria S. Impact of COVID-19 in public health: Prevalence and preventive approaches. Pak J Pharm Sci. 2020;33(4):1739-45. PMid:33583811

Subramaniyan V, Fuloria S, Darnal HK, Meenakshi DU, Sekar M, Nordin RB, et al. COVID-19-associated mucormycosis and treatments. Asian Pac J Trop Med 2021;14(9):401-9. https://doi.org/10.4103/1995-7645.326253 DOI: https://doi.org/10.4103/1995-7645.326253

Huang Q, Zhang S, Huang R, Wei L, Chen Y, Lv S, et al. Isolation and identification of an anti-hepatitis B virus compound from Hydrocotyle sibthorpioides Lam. J Ethnopharmacol. 2013;150(2):568-75. https://doi.org/10.1016/j.jep.2013.09.009 PMid:24051027 DOI: https://doi.org/10.1016/j.jep.2013.09.009

Yu F, Yu F, McGuire PM, Li R, Wang R. Effects of Hydrocotyle sibthorpioides extract on transplanted tumors and immune function in mice. Phytomedicine. 2007;14(2-3):166-71. https://doi.org/10.1016/j.phymed.2006.03.010 PMid:16730434 DOI: https://doi.org/10.1016/j.phymed.2006.03.010

Badrunasar A, Santoso H. In: Rachman E, Siarudin M, editors. Tumbuhan Liar Berkhasiat Obat. Jawa Barat: Forda Press; 2016.

Nazmi R. Anti-inflammatory Effect Test of Extract Ethanol Pegagan Embun (Hydrocotyle sibthorpioides Lam.) with Granuloma Pouch Method or Uji Efek Antiinflamasi Ekstrak Etanol Herba Pegagan Embun (Hydrocotyle sibthorpioides Lam.) dengan Metode Granuloma Pouch., (Bachelor Thesis, Universitas Andalas); 2020.

Afriwardi, Nazmi R, Dillasamola D, Badriyya E, Aldi Y. Anti-inflammatory activity of Pegagan Embun (Hydrocotyle sibthorpioides Lam.) plant extract on topical application. Pharmacogn J. 2021;13(2):411-7. DOI: https://doi.org/10.5530/pj.2021.13.52

Afriwardi, Aldi Y, Dillasamola D, Larakhansa YA, Badriyya E. Immunostimulatory activities of pegagan embun (Hydrocotyle sibthorpioides Lam.) in white male mice. Pharmacogn J. 2021;13(2):368-75. DOI: https://doi.org/10.5530/pj.2021.13.47

Afriwardi, Fitri A, Husni E, Badriyya E, Aldi Y. Immunostimulant Activity of Pegagan Embun Herbs Extract (Hydrocotyle sibthorpioides Lam.) with carbon clearance method towards male white mice. Pharmacogn J. 2021;13(6):1472-7. DOI: https://doi.org/10.5530/pj.2021.13.187

Husni E, Dillasamola D, Badriyya E, Angelia R, Aldi Y. Ethanol extract activity of pegagan embun (Hydrocotyle sibthorpioides L.) against hematopoietic on anemic male white mice. Pharmacogn J. 2021;13(4):866-73. https://doi.org/10.5530/pj.2021.13.111 DOI: https://doi.org/10.5530/pj.2021.13.111

Jola C. Chapter four empirical research in an embodied practice: Can the study of partnering touch, touch on something new? Thinking Touch in Partnering and Contact Improvisation: Philosophy, Pedagogy, Practice. Newcastle upon Tyne, United Kingdom: Cambridge Scholars Publishing; 2020. p. 52.

Suckow MA, Stevens KA, Wilson RP, editors. The Laboratory Rabbit, Guinea Pig, Hamster, and other Rodents. Cambridge, Massachusetts: Academic Press; 2012.

Minstry of Health, Republic Indonesia or Depkes RI. Suplemen III Farmakope Herbal Indonesia. 1st ed. Jakarta. Kementrian Kesehatan RI; 2013.

Abbas AK, Lichtman AH, Pillai S. Imunologi Dasar Abbas: Fungsi dan Kelainan Sistem Imun. 6th ed. Amsterdam, Netherlands: Elsevier; 2020. p. 15-8.

Bruunsgaard H, Skinhøj P, Pedersen AN, Schroll M, Pedersen BK. Ageing, tumour necrosis factor-alpha (TNF-α) and atherosclerosis. Clin Exp Immunol. 2000;121(2):255-60. https://doi.org/10.1046/j.1365-2249.2000.01281.x PMid:10931139 DOI: https://doi.org/10.1046/j.1365-2249.2000.01281.x

Hutasuhut F.Low Immunity Response on Elders. Seri Kesehatan (Health Series). 2006;10(1):10.

Orange JS, Ballas ZK. Natural killer cells in human health and disease. Clin Immunol. 2006;118(1):1-10. https://doi.org/10.1016/j.clim.2005.10.011 PMid:16337194 DOI: https://doi.org/10.1016/j.clim.2005.10.011

Baratawidjaja KG, Rengganis I. Imunologi Infeksi: Imunologi Dasar. 8th ed. Jakarta: Balai Penerbit FKUI; 2009. p. 399-449.

Kumar V, Cotran R, Robbins S. In: Prasetyo A, Pendit B, Priliono T, editors. Buku Ajar Patologi. Jakarta: EGC Penerbit Buku Kedokteran; 2007.

Lie G, Fan Y, Lai Y, Han T, Li Z, Zhou P. Coronavirus infections and immune responses. J Med Virol. 2020;92(4):424-32. https://doi.org/10.1002/jmv.25685 PMid:31981224 DOI: https://doi.org/10.1002/jmv.25685