Experimental Glass Ionomer Cement Containing Gallic acid: Antibacterial Effect and Fluoride Release an in vitro Study

Saher M. Elsharkawy; Yasser F. Gomaa; Reem Gamal

doi:10.3889/oamjms.2022.8694

Authors

Saher M. Elsharkawy Department of Biomaterials, Faculty of Dentistry, Beni-Suef University, Beni-suef, Egypt
Yasser F. Gomaa Faculty of Dentistry, Minia University, Minya, Egypt; 3Department of Biomaterials, Faculty of Dentistry, Minia University, Minya, Egypt
Reem Gamal Department of Biomaterials, Faculty of Dentistry, Minia University, Minya, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.8694

Keywords:

Gallic acid, Glass ionomer cement, Streptococcus mutans, Fluoride release, Working and setting time

Abstract

Abstract:

Aim: Assessment of antibacterial effect and fluoride release on glass ionomer cement (GIC) modified by different concentrations of gallic acid (GA) and their effect on working and setting time.

Methodology: Four groups were tested, group I (control non modified group) and groups II-IV represent GIC modified by GA in three different concentrations (125mg/ml, 62.5mg/ml and 31.25mg/ml GA powder / GIC liquid respectively). Antibacterial effect against Streptococcus Mutans (S. Mutans) was determined after 24hr by broth dilution method. Fluoride release was evaluated after three time intervals 24, 48 and 96hr using spectrophotometer. Working and setting time were measured in all groups by indentation method.

Results: Increasing the concentration of GA significantly increase the antibacterial effect. For all time intervals, the highest fluoride release were observed in group IV and the lowest were in group I. After 24hr groups II, III and IV were significant to group I, while after 48 and 96hr group IV was significant to group I. In addition, working and setting time significantly increased with increasing the GA concentration.

Conclusion: Gallic acid improve the antibacterial effect of GIC against S. Mutans and also improve the fluoride release. The increase in working and setting time of GA modified groups were still within the limit given by ISO 9917–1:2007 specifications.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Plum Analytics Artifact Widget Block

References

Heng C. Tooth decay is the most prevalent disease. Fed Pract. 2016;33(10):31-3. PMid:30766141

Leal S, Bonifacio C, Raggio D, Frencken J. Atraumatic restorative treatment: Restorative component. Monogr Oral Sci. 2018;27:92-102. https://doi.org/10.1159/000487836 PMid:29794453 DOI: https://doi.org/10.1159/000487836

John F. McCabe, Yan Z, Al Naimi OT, Mahmoud G, Rolland SL. Smart materials in dentistry. Aust Dent J. 2011;56(1):3-10. DOI: https://doi.org/10.1111/j.1834-7819.2010.01291.x

Botelho MG. Inhibitory effects on selected oral bacteria of antibacterial agents incorporated in a glass ionomer cement. Caries Res. 2003;37:108-14. https://doi.org/10.1159/000069019 PMid:12652048 DOI: https://doi.org/10.1159/000069019

Khoroushi M, Keshani F. A review of glass-ionomers: From conventional glass-ionomer to bioactive Glass-ionomer. Dent Res J (Isfahan). 2013;10(4):411-20. PMid:24130573

Takahashi Y, Imazato S, Kaneshiro AV, Ebisu S, Frencken JE, Tay FR. Antibacterial effects and physical properties of glass-ionomer cements containing chlorhexidine for the ART approach. Dent Mater. 2006;22(7):647-52. https://doi.org/10.1016/j.dental.2005.08.003 PMid:16226806 DOI: https://doi.org/10.1016/j.dental.2005.08.003

Moshaverinia A, Ansari S, Moshaverinia M, Roohpour N, Darr JA, Rehman I. Effects of incorporation of hydroxyapatite and fluoroapatite nanobioceramics into conventional glass ionomer cements. Acta Biomater. 2008;4(2):432-40. https://doi.org/10.1016/j.actbio.2007.07.011 PMid:17921077 DOI: https://doi.org/10.1016/j.actbio.2007.07.011

Paiva L, Fidalgo TK, da Costa LP, Maia LC, Balan L, Anselme K, et al. Antibacterial properties and compressive strength of new one-step preparation silver nanoparticles in glass ionomer cements (NanoAg-GIC). J Dent. 2018;69:102-9. https://doi.org/10.1016/j.jdent.2017.12.003 PMid:29253621 DOI: https://doi.org/10.1016/j.jdent.2017.12.003

Apurva M, Ramesh KP, Natesan M. Antibacterial effect and physical properties of chitosan and chlorhexidine-cetrimide modified glass ionomer cements. J Indian Soc Pedod Prev Dent. 2017;35(1):28-33. https://doi.org/10.4103/0970-4388.199224 PMid:28139479 DOI: https://doi.org/10.4103/0970-4388.199224

Elgamily H, Ghallab O, El-Sayed H, Nasr M. Antibacterial potency and fluoride release of a glass ionomer restorative material containing different concentrations of natural and chemical products: An in-vitro comparative study. J Clin Exp Dent. 2018;10(4):e312-20. https://doi.org/10.4317/jced.54606 PMid:29750090 DOI: https://doi.org/10.4317/jced.54606

Yoon CH, Chung SJ, Lee SW, Park YB, Lee SK, Park MC. Gallic acid, a natural polyphenolic acid, induces apoptosis and inhibits proinflammatory gene expressions in rheumatoid arthritis fibroblast-like synoviocytes. Joint Bone Spine. 2013;80(3):274-9. https://doi.org/10.1016/j.jbspin.2012.08.010 PMid:23058179 DOI: https://doi.org/10.1016/j.jbspin.2012.08.010

Li K, Guilin G, Junxiang Z. Antibacterial activity and mechanism of a laccase catalyzed chitosan gallic acid derivatives against Esherichia coli and Staphylococcus aureus. J Food Control. 2018;9(2):234-43. DOI: https://doi.org/10.1016/j.foodcont.2018.09.021

Anchana C, Aphiwat T, Rakariyatham N. Antimicrobial gallic acid from Caesalpinia mimosoides Lamk. J Food Chem. 2007;100(3):1044-8. DOI: https://doi.org/10.1016/j.foodchem.2005.11.008

Kang MS, Oh JS, Kang IC, Hong SJ, Choi CH. Inhibitory effect of methyl Gallate and Gallic acid on oral bacteria. J Microbiol. 2008;46(6):744-50. DOI: https://doi.org/10.1007/s12275-008-0235-7

Mazzaoui SA, Burrow MF, Tyas MJ. Fluoride release from glass ionomer cements and resin composites coated with a dentin adhesive. Dent Mater. 2000;16(3):166-71. https://doi.org/10.1016/s0109-5641(00)00003-8 PMid:10762676 DOI: https://doi.org/10.1016/S0109-5641(00)00003-8

Schriks MC, van Amerongen WE. Atraumatic perspectives of ART: Psychological and physiological aspects of treatment with and without rotary instruments. Community Dent Oral Epidemiol. 2003;31(1):15-20. DOI: https://doi.org/10.1034/j.1600-0528.2003.00021.x

Borges A, Ferreira C, Saavedra MJ, Simoes M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug Resist. 2013;19(4):256-65. https://doi.org/10.1089/mdr.2012.0244 PMid:23480526 DOI: https://doi.org/10.1089/mdr.2012.0244

Shao D, Li J, Tang R. Inhibition of gallic acid on the growth and biofilm formation of Escherichia coli and Streptococcus mutans. J Food Sci. 2015;80(6):1299-305. https://doi.org/10.1111/1750-3841.12902 PMid:25974286 DOI: https://doi.org/10.1111/1750-3841.12902

Kang J, Liu L, Liu M, Wu X, Li J. Antibacterial activity of Gallic acid against and its effect on biofilm Shigella flexneri formation by repressing gene expression. J Food Control. 2018;7(1):147-54. DOI: https://doi.org/10.1016/j.foodcont.2018.07.011

Lemos JA, Palmer SR, Zeng L, Abranches J. The biology of Streptococcus mutans. Microbiol Spectr. 2019;7(1):1110-28. DOI: https://doi.org/10.1128/microbiolspec.GPP3-0051-2018

Balouiri M, Sadiki M, Koraichi S. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016;6(2):71-9. https://doi.org/10.1016/j.jpha.2015.11.005 PMid:29403965 DOI: https://doi.org/10.1016/j.jpha.2015.11.005

Divya KP, Khijmatgar S, Chowdhury A. Factors influencing fluoride release in atraumatic restorative treatment (ART) materials: A review. J Oral Biol Craniofac Res. 2019;9(4):315-20. https://doi.org/10.1016/j.jobcr.2019.06.015 PMid:31334004 DOI: https://doi.org/10.1016/j.jobcr.2019.06.015

Wiegand A, Buchalla W, Attin T. Review on fluoride releasing restorative materials, fluoride release and uptake characteristics, antibacterial activity and influence on caries formation. Dent Mater. 2007;23:343-62. https://doi.org/10.1016/j.dental.2006.01.022 PMid:16616773 DOI: https://doi.org/10.1016/j.dental.2006.01.022

Jackson GE. The existence of AlF4− in aqueous solution and its relevance to phosphorylase reaction. Inorg Chem Acta. 1988;151(4):273-6. DOI: https://doi.org/10.1016/S0020-1693(00)90812-0

Sidhu SK, Nicholson JW. A review of glass-ionomer cements for cinical dentistry. J Funct Biomater. 2016;7(3):16. https://doi.org/10.3390/jfb7030016 PMid:27367737 DOI: https://doi.org/10.3390/jfb7030016

Attar N, Turgut MD. Fluoride release and uptake capacities of fluoride-releasing restorative materials. Oper Dent. 2003;28(4):395-402. PMid:12877425

Neelakantan P, John S, Anand S, Sureshbabu N, Subbarao C. Fluoride release from a new Glass-ionomer cement. Oper Dent. 2011;36(1):80-5. https://doi.org/10.2341/10-219-LR PMid:21488733 DOI: https://doi.org/10.2341/10-219-LR

Craig R, Powers J. Restorative Dental Materials. 14th ed. London, UK: Mosby, Elsevier; 2019.

Anusavice KJ, Shen C, Rawls R. Phillips’ Science of Dental Materials. 13th ed. Amsterdam, Netherlands: Elsevier; 2021.

Prosser HJ, Richards CP, Wilson AD. NMR spectroscopy of dental cements II. The role of tartaric acid in Glass-Lonomer cements. J Biomed Mater Res. 1982;16(4):431-45. DOI: https://doi.org/10.1002/jbm.820160411

Nicholson JW, Brookman PJ, Lacy OM, Wilson AD. Fourier transform infrared spectroscopic study of the role of tartaric acid in Glass-Ionomer dental cements. J Dent Res. 1988;67(12):1451-4. DOI: https://doi.org/10.1177/00220345880670120201