Antibacterial Effects of Steeped White Tea, Black Tea, and Green Tea against Streptococcus mutans and Plaque Accumulation
DOI:
https://doi.org/10.3889/oamjms.2022.8698Keywords:
Streptococcus mutans, Plaque, White Tea, Black Tea, Green TeaAbstract
BACKGROUND: Tea is the most consumed beverage in the world after water. Tea (Camellia sinensis) is native to Southeast Asia and is now available in more than 30 countries. Tea has various health properties. Tea has an active component, namely catechins, which can inhibit the growth of Streptococcus mutans as a component of dental plaque formation.
AIM: This study aims to determine the antibacterial effect of steeped white tea, black tea, and green tea on S. mutans and plaque accumulation.
METHODS: The research used a quasi-experimental design with pre-test and post-test group design. The population was students of the Department of Dental Nursing. Purposive sampling technique is used with a sample size of 48 students who were divided into three parts of the sample group for the 2.5% concentration of tea mouthwash treatment. The analysis used paired t-test to see the antibacterial effect and differences in three-variable test followed by Kruskal–Wallis test.
RESULTS: The results showed that the mouth rinses of steeped white tea, black tea, and green tea were effective in reducing S. mutans and plaque accumulation on teeth. There is a significant difference in the inhibition zone of S. mutans against the three types of tea, and white has the largest inhibition zone. As for plaque accumulation, there is no difference between the three types of tea.
CONCLUSIONS: It can be concluded that mouth rinsing with steeping white tea, black tea, and green tea with a concentration of 2.5% has antibacterial properties that can inhibit S. mutans and plaque accumulation.Downloads
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Haryani N, Mahmiyah E, Ayatullah MI. The use of turmeric (Extracts and Squeeze) to detect plaque on teeth. J Health Info. 2019;17(2):169-75. https://doi.org/10.31965/infokes.Vol17.Iss2.276 DOI: https://doi.org/10.31965/infokes.Vol17.Iss2.276
Abdulbaqi HR, Himratul-Aznita WH, Baharuddin NA. Evaluation of Salvadora persica L. and green tea anti-plaque effect: A randomized controlled crossover clinical trial. BMC Complement Alter Med. 2016;16(1):493. https://doi.org/10.1186/s12906-016-1487-0 PMid:27903262 DOI: https://doi.org/10.1186/s12906-016-1487-0
Newman MG, Takei H, Klokkevold PR, Carranza, FA. Carranza’s Clinical Periodontology. Amsterdam, Netherlands: Elsevier Health Sciences; 2011. https://doi.org/10.1016/b978-1-4377-0416-7.00109-8 DOI: https://doi.org/10.1016/B978-1-4377-0416-7.00109-8
Boel T. The antibacterial power of the combination of triclosan and zinc citrate in several concentrations on the growth of mutans streptococci. Dentika Dent J. 2000;5(1):7-10.
Patabang W, Leman MA, Maryono J. The differences in the number of bacterial colonies growing oral cavity before and after using mouthwash containing chlorheksidine. Pharmacon. 2016;5(1):26-31. https://doi.org/10.47522/jmk.v2i2.44 DOI: https://doi.org/10.47522/jmk.v2i2.44
Malhotra R, Grover V, Kapoor A, Saxena D. Comparison of the effectiveness of a commercially available herbal mouthrinse with chlorhexidine gluconate at the clinical and patient level. J Indian Soc Periodontol. 2011;15(4):349-52. https://doi.org/10.4103/0972-124X.92567 PMid:22368358 DOI: https://doi.org/10.4103/0972-124X.92567
Tenore G, Daglia M, Ciampaglia R, Novellino E. Exploring the nutraceutical potential of polyphenols from black, green and white tea infusions-an overview. Curr Pharm Biotechnol. 2015;16(3):265-71. https://doi.org/10.2174/1389201016666150118133604 PMid:25601602 DOI: https://doi.org/10.2174/1389201016666150118133604
Amalia N, Widodo SK. Comparison of the effectiveness of gargling white tea solution (Camellia sinensis L.) brewing 100% dose with 50% in increase the Ph of saliva. J Dent. 2014;2(1):29-33.
Moghbel A, Farjzadeh A, Aghel N, Aghel H, Raisi N. Evaluation of the effect of green tea extract on mouth bacterial activity in the presence of propylene glycol. Jundishapur J Nat Pharm Prod. 2012;7(2):56-60. https://doi.org/10.5812/jjnpp.3588 PMid:24624155 DOI: https://doi.org/10.17795/jjnpp-3588
Subramaniam P, Eswara U, Reddy KR. Effect of different types of tea on Streptococcus mutans: An in vitro study. Indian J Dent Res. 2012;23(1):43-8. https://doi.org/10.4103/0970-9290.99037 DOI: https://doi.org/10.4103/0970-9290.99037
Insanu M, Maryam I, Rohdiana D, Wirasutisna KR. Antibacterial activity test of fifteen types of quality of rotorvane and orthodox black tea white tea (Camellia sinensis var. assamica) on Staphylococcus aureus atcc 6538. Acta Pharm Indones. 2017;42(1):32-41. https://doi.org/10.1021/acs.jafc.1c08178.s001 DOI: https://doi.org/10.1021/acs.jafc.1c08178.s001
Barroso H, Ramalhete R, Domingues A, Maci S. Inhibitory activity of a green and black tea blend on Streptococcus mutans. J Oral Microbiol. 2018;10(1):1481322. https://doi.org/10.1080/20002297.2018.1481322 PMid:29887975 DOI: https://doi.org/10.1080/20002297.2018.1481322
Muniandy P, Shori AB, Baba US. Influence of green, white and black tea addition on the antioxidant activity of probiotic yogurt during refrigerated storage. Food Packag Shelf Life. 2016;8:1-8. https://doi.org/10.1016/j.fpsl.2016.02.002 DOI: https://doi.org/10.1016/j.fpsl.2016.02.002
Leung LK, Su Y, Chen R, Zhang Z, Huang Y, Chen ZY. Theaflavins in black tea and catechins in green tea are equally effective antioxidants. J Nutr. 2001;131(9):2248-51. https://doi.org/10.1093/jn/131.9.2248 PMid:11533262 DOI: https://doi.org/10.1093/jn/131.9.2248
Ferrara L, Montesano D, Senatore A. The distribution of minerals and flavonoids in the tea plant (Camellia sinensis). Farmaco. 2019;56(5- 7):397-401. https://doi.org/10.1016/S0014-827X(01)01104-1 PMid:11482766 DOI: https://doi.org/10.1016/S0014-827X(01)01104-1
Azizi S, Shahri MM, Rahman HS, Rahim RA, Rasedee A, Mohamad R. Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line. Int J Nanomed. 2017;12:8841. https://doi.org/10.2147/IJN.S149371 PMid:29276385 DOI: https://doi.org/10.2147/IJN.S149371
Takemoto M, Takemoto H. Synthesis of theaflavins and their functions. Molecules. 2018;23(4):918. https://doi.org/10.3390/molecules23040918 PMid:29659496 DOI: https://doi.org/10.3390/molecules23040918
Kaur H, Jain S, Kaur A. Comparative evaluation of the antiplaque effectiveness of green tea catechin mouthwash with chlorhexidine gluconate. J Indian Soc Periodontol. 2014;18(2):178-82. https://doi.org/10.4103/0972-124X.131320 PMid:24872625 DOI: https://doi.org/10.4103/0972-124X.131320
Suma FP, Dewi N, Adhani R. The Effectiveness of black tea (Camellia sinensis) in dental plaque index decline. (Review in Students junior hight school 2 Banjarbaru).Dentino. 2016;1(2):79-83. https://doi.org/10.35910/jbkm.v5i1.450 DOI: https://doi.org/10.35910/jbkm.v5i1.450
Shinta LS, Kawengian SE, Mariati NW. The effectiveness of gargling with green tea brewed water in reducing plaque accumulation. E-GiGi. 2015;3(2):9834. https://doi.org/10.35790/eg.3.2.2015.9834 DOI: https://doi.org/10.35790/eg.3.2.2015.9834
Thomas A, Thakur S, Habib R. Comparison of antimicrobial efficacy of green tea, garlic with lime, and sodium fluoride mouth rinses against Streptococcus mutans, Lactobacilli species, and Candida albicans in children: A randomized double-blind controlled clinical trial. Int J Clin Pediatr Dent. 2017;10(3):234-9. https://doi.org/10.5005/jp-journals-10005-1442 PMid:29104381 DOI: https://doi.org/10.5005/jp-journals-10005-1442
Okamoto M, Leung KP, Ansai T, Sugimoto A, Maeda N. Inhibitory effects of green tea catechins on protein tyrosine phosphatase in prevotella intermedia. Oral Microbiol Immunol. 2003;18(3):192-5. https://doi.org/10.1034/j.1399-302X.2003.00056.x PMid:12753472 DOI: https://doi.org/10.1034/j.1399-302X.2003.00056.x
Neturi RS, Srinivas R, Simha BV, Sree YS, Shekar TC, Kumar S. Effects of green tea on Streptococcus mutans counts-a randomized control trail. J Clin Diagn Res. 2014;8(11):128-30. https://doi.org/10.7860/JCDR/2014/10963.5211 PMid:25584303 DOI: https://doi.org/10.7860/JCDR/2014/10963.5211
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Copyright (c) 2022 Damhuji Damhuji, Budi Suryana, M. Ibraar Ayatullah (Author)
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