The Effect of Gambier Catechin Isolate on Cervical Cancer Cell Death (HeLa Cell Lines)
DOI:
https://doi.org/10.3889/oamjms.2022.8779Keywords:
Cervical cancer, Catechin, Apoptosis, HeLa cell line, Cell deathAbstract
BACKGROUND: Cervical cancer is the second most common type of cancer in women worldwide. Human Papilloma Virus infection on the surface of the cervix is the most common cause which can cause abnormal growth of cervical cells.
AIM: This research was conducted in vitro which aims to determine whether catechin compounds can inhibit the growth and regulation of cervical cancer cells (HeLa cell line).
METHODS: This is experimental research using the colourimetric assay method and qualitative observation of cervical cancer cell morphology (HeLa cell line) under a fluorescence microscope. The administration of catechin compounds was tested at different concentrations to HeLa cells, namely 1000 g/ml, 500 g/ml, 250 g/ml, 125 g/ml, 62.5 g/ml, 31.25 g/ml.
RESULTS: The smallest cell viability was obtained from a concentration of 1000 g/ml which was 5.98% while the largest cell viability was found at a concentration of 31.25 g/ml, which was 40.01%. The resulting IC50 value was 22.91 g/ml. Gambier catechin compounds have very high antioxidants because they contain an IC50 value < 50 g/ml. The effect of gambier catechin compounds on HeLa cell death can be found by increasing the percentage of dead cells. The difference in the fluorescence images of HeLa cells in the experimental group was assessed based on the percentage of the number of cells that died or underwent apoptosis, which was marked by a red or orange fluorescent image. At the concentration of IC25, 31.87% of dead cells were found, the concentration of IC50 was 51.09% of dead cells, and the concentration of IC75 was 82.51% of dead cells. The test results showed that there was a significant difference in the average percentage of cells undergoing apoptosis in all study groups with p <0.05.
CONCLUSION: Based on research, it can be concluded that catechin compounds could inhibit the growth and regulation of cervical cancer cells (HeLa cell line). Later, it has the potential to be developed as an anticancer candidate for cervical cancer.
Keyword: Cervical cancer, Catechin, Apoptosis, HeLa cell line, Cell death.
Downloads
Metrics
Plum Analytics Artifact Widget Block
References
Chan CK, Aimagambetova G, Ukybassova T, Kongrtay K, Azizan A. Human Papillomavirus infection and cervical cancer: Epidemiology, screening, and vaccinatio-review of current perspectives. J Oncol. 2019;2019:3257939. https://doi.org/10.1155/2019/3257939 PMid:31687023 DOI: https://doi.org/10.1155/2019/3257939
Hull R, Mbele M, Makhafola T, Hicks C, Wang SM, Reis RM, et al. Cervical cancer in low and middle-income countries. Oncol Lett. 2020;20(3):2058-74. https://doi.org/10.3892/ol.2020.11754 PMid:32782524 DOI: https://doi.org/10.3892/ol.2020.11754
The Global Cancer Observatory. Cancer Incident in Indonesia. Vol. 858. Lyon, France: International Agency for Research on Cancer; 2020. Available from: https://gco.iarc.fr [Last accessed 2021 Aug 29].
Yang B, Chen Y, Shi J. Tumor-specific chemotherapy by nanomedicine-enabled differential stress sensitization. Angew Chem Int Ed. 2020;59(24):9693-701. https://doi.org/10.1002/anie.202002306 DOI: https://doi.org/10.1002/anie.202002306
Li XM, Luo XG, He JF, Wang N, Zhou H, Yang PL, et al. Induction of apoptosis in human cervical carcinoma hela cells by active compounds from Hypericum ascyron L. Oncol Lett. 2018;15(3):3944-50. https://doi.org/10.3892/ol.2018.7812 PMid:29556280 DOI: https://doi.org/10.3892/ol.2018.7812
Kibiti C, Afolayan A. Herbal therapy: A review of emerging pharmacological tools in the management of diabetes mellitus in Africa. Pharmacogn Mag. 2015;11(44):258-74. https://doi.org/10.4103/0973-1296.166046 DOI: https://doi.org/10.4103/0973-1296.166046
Hosen N, Pengkajian B, Pertanian T, Barat BS. Profile of the gambier farming system in West Sumatra. J Penelit Pertan Terap. 2017;17(2):124-31. https://doi.org/10.25181/jppt.v17i2.291 DOI: https://doi.org/10.25181/jppt.v17i2.291
Cheng Z, Zhang Z, Han Y, Wang J, Wang Y, Chen X, et al. A review on anti-cancer effect of green tea catechins. J Funct Foods. 2020;74:104172. https://doi.org/10.1016/j.jff.2020.104172 DOI: https://doi.org/10.1016/j.jff.2020.104172
Khiewkamrop P, Phunsomboon P, Richert L, Pekthong D, Srisawang P. Epistructured catechins, EGCG and EC facilitate apoptosis induction through targeting de novo lipogenesis pathway in HepG2 cells. Cancer Cell Int. 2018;18:46. https://doi.org/10.1186/s12935-018-0539-6 DOI: https://doi.org/10.1186/s12935-018-0539-6
Khojaste E, Ahmadizadeh C. Catechin Metabolites along with curcumin inhibit proliferation and induce apoptosis in cervical cancer cells by regulating VEGF expression in-vitro. Nutr Cancer. 2022;74(3):1048-57. https://doi.org/10.1080/01635581.2021.1936082 PMid:34121550 DOI: https://doi.org/10.1080/01635581.2021.1936082
Zhu Y, Huang Y, Liu M, Yan Q, Zhao W, Yang P, et al. Epigallocatechin gallate inhibits cell growth and regulates miRNA expression in cervical carcinoma cell lines infected with different highrisk human papillomavirus subtypes. Exp Ther Med. 2019;17(3):1742-8. https://doi.org/10.3892/etm.2018.7131 PMid:30783443 DOI: https://doi.org/10.3892/etm.2018.7131
Cancer Chemoprevention Research Center. Protokol Uji Sitotoksik Metode Mtt. Indonesia: Cancer Chemoprevention Research Center Fakultas Farmasi UGM; 2013.
Anggraini T, Rini, Aini L. The extraction of gambier by using ultrasonic assisted at various temperature. IOP Conf Ser Earth Environ Sci. 2021;757(2021):012058. https://doi.org/10.1088/1755-1315/757/1/012058 DOI: https://doi.org/10.1088/1755-1315/757/1/012058
Polite BN, Adams-Campbell LL, Brawley OW, Bickell N, Carethers JM, Flowers CR, et al. Charting the future of cancer health disparities research: A position statement from the American association for cancer research, the American cancer society, the American society of clinical oncology, and the national cancer institute. CA Cancer J Clin. 2017;67(5):353-61. https://doi.org/10.3322/caac.21404 PMid:28738442 DOI: https://doi.org/10.3322/caac.21404
Saganuwan SA. Application of modified Michaelis-Menten equations for determination of enzyme inducing and inhibiting drugs. BMC Pharmacol Toxicol. 2021;22(1):57. https://doi.org/10.1186/s40360-021-00521-x PMid:34635182 DOI: https://doi.org/10.1186/s40360-021-00521-x
Wang YQ, Lu JL, Liang YR, Li QS. Suppressive effects of EGCG on cervical cancer. Molecules. 2018;23(9):2334. https://doi.org/10.3390/molecules23092334 PMid:30213130 DOI: https://doi.org/10.3390/molecules23092334
Chakrabarty S, Ganguli A, Das A, Nag D, Chakrabarti G. Epigallocatechin-3-gallate shows anti-proliferative activity in HeLa cells targeting tubulin-microtubule equilibrium. Chem Biol Interact. 2015;242:380-9. https://doi.org/10.1016/j.cbi.2015.11.004 PMid:26554336 DOI: https://doi.org/10.1016/j.cbi.2015.11.004
Tudoran O, Soritau O, Balacescu O, Balacescu L, Braicu C, Rus M, et al. Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells. J Cell Mol Med. 2012;16(3):520-30. https://doi.org/10.1111/j.1582-4934.2011.01346.x PMid:21609393 DOI: https://doi.org/10.1111/j.1582-4934.2011.01346.x
Azam S, Hadi N, Khan NU, Hadi SM. Prooxidant property of green tea polyphenols epicatechin and epigallocatechin-3- gallate: Implications for anticancer properties. Toxicol In Vitro. 2004;18(5):555-61. https://doi.org/10.1016/j.tiv.2003.12.012 PMid:15251172 DOI: https://doi.org/10.1016/j.tiv.2003.12.012
Krstic M, Stojadinovic M, Smiljanic K, Stanic-Vucinic D, Velickovic TC. The anti-cancer activity of green tea, coffee and cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both pro-oxidant and anti-proliferative activities of polyphenols. RSC Adv. 2015;5(5):3260-8. https://doi.org/10.1039/c4ra13230k DOI: https://doi.org/10.1039/C4RA13230K
Downloads
Published
How to Cite
License
Copyright (c) 2022 Refa Rahmaddiansyah, Suci Hasani, Azizah Amatu Zikrah, Dessy Arisanty (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
http://creativecommons.org/licenses/by-nc/4.0
