Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria

Authors

  • Alina Petruk Department of Infectious Diseases and Epidemiology, Dermatology and Venereology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Iryna Kamyshna Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine https://orcid.org/0000-0002-4483-1856
  • Mariia Shkilna Department of Infectious Diseases and Epidemiology, Dermatology and Venereology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Alexandr M. Kamyshnyi Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine https://orcid.org/0000-0003-3141-4436

DOI:

https://doi.org/10.3889/oamjms.2022.8781

Keywords:

Acute urticaria, mRNA, Innate and adaptive immune responses

Abstract

OBJECTIVE: Alterations in the transcriptional activity of some immunoregulatory genes can play a key in the pathogenesis of acute urticarial (AU). Minimally-invasive markers of the transcriptional activity of immune response genes are essential not only for predicting the severity and activity of the disease but also as a potential target for therapy.

METHODS: In our research, we applied a pathway-specific polymerase chain reaction PCR array (Human Innate and Adaptive Immune Responses RT2 Profiler PCR Array, QIAGEN, Germany) to detect and verify innate and adaptive immune responses pathway-focused genes expression in patients suffering from AU and control group.

RESULTS: The AU development was accompanied by an increase in the transcriptional activity of genes for a number of costimulation molecules such as CD40, CD40LG, CD80 (B7-1), and C-reactive protein and myeloperoxidase genes either. Under AU conditions, transcriptional induction of genes of several cytokines was also observed: Interferon gamma, interleukin (IL4), IL5, IL17A, tumor necrosis factor, and also chemokine CXCL8. This process was also accompanied by an increase in the transcriptional activity of the RAR-related orphan receptor C Th17 differentiation regulator, the NLRP3 inflammasome genes, and the NFKB1 transcription factor. Such changes occurred against the background of transcriptional repression of the FOXP3 gene and the Treg-dependent suppressor cytokine IL10. The expression of other studied genes did not differ significantly from the controls.

CONCLUSIONS: The development of acute urticaria led to the transcriptional activation of pro-inflammatory signaling against the background of a deficiency of the suppressor link. Detected changes in gene expression can be important for targeted therapy.

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Published

2022-03-09

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1.
Petruk A, Kamyshna I, Shkilna M, Kamyshnyi AM. Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria. Open Access Maced J Med Sci [Internet]. 2022 Mar. 9 [cited 2024 Apr. 20];10(A):383-9. Available from: https://oamjms.eu/index.php/mjms/article/view/8781

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