Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria


  • Alina Petruk Department of Infectious Diseases and Epidemiology, Dermatology and Venereology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Iryna Kamyshna Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Mariia Shkilna Department of Infectious Diseases and Epidemiology, Dermatology and Venereology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Alexandr M. Kamyshnyi Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine



Acute urticaria, mRNA, Innate and adaptive immune responses


OBJECTIVE: Alterations in the transcriptional activity of some immunoregulatory genes can play a key in the pathogenesis of acute urticarial (AU). Minimally-invasive markers of the transcriptional activity of immune response genes are essential not only for predicting the severity and activity of the disease but also as a potential target for therapy.

METHODS: In our research, we applied a pathway-specific polymerase chain reaction PCR array (Human Innate and Adaptive Immune Responses RT2 Profiler PCR Array, QIAGEN, Germany) to detect and verify innate and adaptive immune responses pathway-focused genes expression in patients suffering from AU and control group.

RESULTS: The AU development was accompanied by an increase in the transcriptional activity of genes for a number of costimulation molecules such as CD40, CD40LG, CD80 (B7-1), and C-reactive protein and myeloperoxidase genes either. Under AU conditions, transcriptional induction of genes of several cytokines was also observed: Interferon gamma, interleukin (IL4), IL5, IL17A, tumor necrosis factor, and also chemokine CXCL8. This process was also accompanied by an increase in the transcriptional activity of the RAR-related orphan receptor C Th17 differentiation regulator, the NLRP3 inflammasome genes, and the NFKB1 transcription factor. Such changes occurred against the background of transcriptional repression of the FOXP3 gene and the Treg-dependent suppressor cytokine IL10. The expression of other studied genes did not differ significantly from the controls.

CONCLUSIONS: The development of acute urticaria led to the transcriptional activation of pro-inflammatory signaling against the background of a deficiency of the suppressor link. Detected changes in gene expression can be important for targeted therapy.


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Pier J, Bingemann TA. Urticaria, angioedema, and anaphylaxis. Pediatr Rev. 2020;41:283-92. DOI:

Radonjic-Hoesli S, Hofmeier KS, Micaletto S, Schmid- Grendelmeier P, Bircher A, Simon D. Urticaria and angioede-ma: An update on classification and pathogenesis. Clin Rev Allergy Immunol. 2018;54(1):88-101. PMid:28748365 DOI:

Folci M, Ramponi G, Brunetta EA. Comprehensive approach to urticaria: From clinical presentation to modern bio-logical treatments through pathogenesis. Adv Exp Med Biol. 2021;1326:111-37. PMid:33385176 DOI:

Marques-Mejías MA, Tomás-Pérez M, Vilà-Nadal G, Quirce S. Acute urticaria in the pediatric emergency department: Management and possible triggers. Ann Allergy Asthma Immunol. 2020;124:396-97. PMid:31981615 DOI:

Bilous I, Pavlovych L, Krynytska I, Marushchak M, Kamyshnyi A. Apoptosis and cell cycle pathway-focused genes expression analysis in patients with different forms of thyroid pathology. Open Access Maced J Med Sci. 2020;8:784-92. DOI:

Bilous II, Korda MM, Krynytska IY, Kamyshnyi AM. Nerve impulse transmission pathway-focused genes expression analysis in patients with primary hypothyroidism and autoimmune thyroiditis. Endocr Regul. 2020;54:109-18. PMid:32597152 DOI:

Bilous II, Pavlovych LB, Kamyshnyi AM. Primary hypothyroidism and autoimmune thyroiditis alter the transcription-al activity of genes regulating neurogenesis in the blood of patients. Endocr Regul. 2021;55:5-15. PMid:33600668 DOI:

Kamyshna II, Pavlovych LB, Maslyanko VA, Kamyshnyi AM. Analysis of the transcriptional activity of genes of neu-ropeptides and their receptors in the blood of patients with thyroid pathology. J Med Life. 2021;14:243-9. PMid:34104248 DOI:

Kamyshna I, Kamyshnyi A. Transcriptional activity of neurotrophins genes and their receptors in the peripheral blood in patients with thyroid diseases in bukovinian population of Ukraine. Open Access Maced J Med Sci. 2021;9:208-16. DOI:

Putilin DA, Evchenko SY, Fedoniuk LY, Tokarskyy OS, Kamyshny OM, Migenko LM, et al. The influence of metformin to the transcriptional activity of the mTOR and FOX3 genes in parapancreatic adipose tissue of streptozotocin-induced diabetic rats. J Med Life. 2020;13(1):50-5. jml-2020-0029 PMid:32341701

Nosulenko IS, Voskoboynik OY, Berest GG, Safronyuk SL, Kovalenko SI, Kamyshnyi OM, et al. Synthesis and antimicrobial activity of 6-thioxo-6, 7-dihydro-2H-[1, 2, 4]triazino[2, 3-c]-quinazolin-2-one derivatives. Sci Pharm. 2014;82(3):483-500. PMid:25853063 DOI:

Topol IA, Kamyshny AM, Abramov AV, Kolesnik YM. Expression of XBP1 in lymphocytes of the small intestine in rats under chronic social stress and modulation of intestinal microflora composition. Fiziol Zh. 2014;60(2):38-44. PMid:25007519 DOI:

Kamyshna I, Pavlovych L, Malyk I, Kamyshnyi A. 25-OH Vitamin D blood serum linkage with VDR gene polymorphism (rs2228570) in thyroid pathology patients in the West-Ukrainian population. J Med Life. 2021;14(4):549-56. PMid:34621381 DOI:

Kamyshna II, Pavlovych LB, Sydorchuk LP, Malyk IV, Kamyshnyi AM. BDNF blood serum linkage with BDNF gene polymorphism (rs6265) in thyroid pathology patients in the West-Ukrainian population. Endocr Regul. 2021;55(4):193-203. PMid:34879183 DOI:

Lin CE, Kaptein JS, Sheikh J. Differential expression of microRNAs and their possible roles in patients with chronic idiopathic urticaria and active hives. Allergy Rhinol. 2017;8:67-80. PMid:28583230 DOI:

Li J, Guo A, Chen W, Bin L, He Y, Zhu W, et al. Association of ORAI1 gene polymorphisms with chronic spontaneous urticaria and the efficacy of the nonsedating H1 antihistamine desloratadine. J Allergy Clin Immunol. 2017;139:1386-e9. PMid:27864023 DOI:

Metz M, Torene R, Kaiser S, Beste MT, Staubach P, Bauer A, et al. Omalizumab normalizes the gene expression signature of lesional skin in patients with chronic spontaneous urticaria: A randomized, double-blind, placebo-controlled study. Allergy. 2019;74:141-51. PMid:29974963 DOI:

Giménez-Arnau A, Curto-Barredo L, Nonell L, Puigdecanet E, Yelamos J, Gimeno R, et al. Transcriptome analysis of severely active chronic spontaneous urticaria shows an overall immunological skin involvement. Allergy. 2017;72:1778-90. PMid:28407332 DOI:

Zhang Y, Zhang H, Du S, Yan S, Zeng J. Advanced biomarkers: Therapeutic and diagnostic targets in urticaria. Int Arch Allergy Immunol. 2021;29:1-15. PMid:33915552 DOI:

Sarkar TK, Sil A, Pal S, Ghosh C, Das NK. Effectiveness and safety of levocetirizine 10 mg versus a combination of levocetirizine 5 mg and montelukast 10 mg in chronic urticaria resistant to levocetirizine 5 mg: A double-blind, random-ized, controlled trial. Indian J Dermatol Venereol Leprol. 2017;83:561-8. PMid:28656910 DOI:

Wang X, Liu J, Song S, Xu B, Tan X, Si S. Detection and analysis of toll-like receptors and DC-SIGN expression in peripheral blood mononuclear cells from patients with chronic urticaria. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014;30:736-9. PMid:25001940

Giménez-Arnau AM, DeMontojoye L, Asero R, Cugno M, Kulthanan K, Yanase Y, et al. The patho-genesis of chronic spontaneous urticaria: The role of infiltrating cells. J Allergy Clin Immunol Pract. 2021;9:2195-208. DOI:

Deza G, Ricketti PA, Gimenez-Arnau AM, Casale TB. Emerging biomarkers and therapeutic pipelines for chronic spontaneous urticaria. J Allergy Clin Immunol Pract. 2018;6:1108-17. PMid:30033912 DOI:

Toubi E, Vadasz Z. The emerging role of IL-17 in the immune-pathogenesis of chronic spontaneous urticaria. Immunotargets Ther. 2020;9:217-23. PMid:33134229 DOI:

Sabag DA, Matanes L, Bejar J, Sheffer H, Barzilai A, Church MK, et al. Interleukin-17 is a potential player and treatment target in severe chronic spontaneous urticaria. Clin Exp Allergy. 2020;50:799-804. PMid:32412136 DOI:

Grzanka A, Damasiewicz-Bodzek A, Kasperska-Zajac A. The relationship between circulating concentrations of interleu-kin 17 and C reactive protein in chronic spontaneous urticaria. Allergy Asthma Clin Immunol. 2017;10:25-31. PMid:28491102 DOI:

Maurer M, Khan DA, Komi DE, Kaplan AP. Biologics for the use in chronic spontaneous urticaria: When and which. J Allergy Clin Immunol Pract. 2021;9:1067-78. PMid:33685605 DOI:

Atwa MA, Emara AS, Youssef N, Bayoumy NM. Serum concentration of IL-17, IL-23 and TNF-a among patients with chronic spontaneous urticaria: Association with disease activity and autologous serum skin test. J Eur Acad Dermatol Venereol. 2014;28:469-74. PMid:23451767 DOI:

Bangsgaard N, Skov L, Zachariae C. Treatment of refractory chronic spontaneous urticaria with adalimumab. Acta Derm Venereol. 2017;97(4):524-5. PMid:27840884 DOI:

Alasandagutti ML, Ponnana M, Sivangala R, Thada S, Joshi L, Hussain H, et al. Role of IFN-gamma and IL-6 cytokines and their association in determining susceptibility to chronic idiopathic urticaria. Genet Test Mol Biomarkers. 2014;18(12):804-9. PMid:25409241 DOI:

Grieco T, Porzia A, Paolino G, Chello C, Sernicola A, Faina V, et al. IFN-γ/IL-6 and related cytokines in chronic spontaneous urticaria: Evaluation of their pathogenetic role and changes during omali-zumab therapy. Int J Dermatol. 2020;59:590-4. PMid:32048727 DOI:

Degirmenci PB, Kirmaz C, Vatansever S, Onur E, Nal E, Erdin S, Ozyurt B. Analysis of the association of chronic spontaneous urticaria with interlekin-4, -10, transforming growth factor-beta1, interferongamma, interleukin-17A and -23 by autologous serum skin test. Postepy Dermatol Alergol. 2017;34:70-6. PMid:28261034 DOI:

Chen Q, Zhong H, Chen WC, Zhai Z, Zhou Z, Song Z, et al. Different expression patterns of plasma Th1-, Th2-, Th17- and Th22-related cytokines correlate with serum autoreactivity and allergen sensitivity in chronic spontaneous urti-caria. J Eur Acad Dermatol Venereol. 2018;32:441-8. PMid:28846158 DOI:

Lee JK, Simpson RS. Dupilumab as a novel therapy for difficult to treat chronic spontaneous urticaria. J Allergy Clin Immunol Pract. 2019;7:1659-61. PMid:30496828 DOI:

Yu Q, Lin W, Zhang J, Peng L, Kong Q, Zhong R, et al. The association of Th17/Treg cells ex-pression in peripheral blood and chronic spontaneous urticaria: A protocol of systematic review and meta-analysis. Medicine (Baltimore). 2020;99:e22014. PMid:32899053 DOI:

Arshi S, Babaie D, Nabavi M, Tebianian M, Ghalehbaghi B, Jalali F, et al. Circulating level of CD4+ CD25+ FOXP3+ T cells in patients with chronic urticaria. Int J Dermatol. 2014;53:e561-6. PMid:25311400 DOI:

Moos Ł, Kapeluszna K, Okuniewicz R, Brzoza Z. The role of interleukin 10 and 18 in chronic spontaneous urticaria pathogenesis in the context of angioedema coexistence. J. Interferon Cytokine Res. 2021;41:172-6. PMid:34003678 DOI:

Feng H, Feng J, Zhang Z, Xu Q, Hu M, Wu Y, et al. Role of IL-9 and IL-10 in the pathogenesis of chronic spontaneous urticaria through the JAK/STAT signalling pathway. Cell Biochem Funct. 2020;38:480-9. PMid:31989663 DOI:

Patel OP, Giorno RC, Dibbern DA, Andrews KY, Durairaj S, Dreskin SC. Gene expression profiles in chronic idio-pathic (spontaneous) urticaria. Allergy Rhinol. 2015;6:101-10. PMid:26302730 DOI:

Santos JC, de Brito CA, Futata EA, Azor MH, Orii NM, Maruta CW, et al. Up-regulation of chemokine C-C ligand 2 (CCL2) and C-X-C chemokine 8 (CXCL8) expression by monocytes in chronic id-iopathic urticaria. Clin Exp Immunol. 2012;167:129-36. PMid:22132892 DOI:




How to Cite

Petruk A, Kamyshna I, Shkilna M, Kamyshnyi AM. Analysis of the Transcriptional Activity of Immune Response Genes in the Blood of Patients with Acute Urticaria. Open Access Maced J Med Sci [Internet]. 2022 Mar. 9 [cited 2023 Mar. 31];10(A):383-9. Available from:

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