Optimization of the Duration of the Administration of Mesenchymal Stem Cells Wharton’s Jelly to the Level of Matrix Metalloproteinase-1 and Transforming Growth Factor-β in Osteoarthritis Rat Model


  • Endrinaldi Endrinaldi Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Hirowati Ali Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Tofrizal Tofrizal Department of Anatomical Pathology, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Asterina Asterina Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Elmatris Elmatris Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia
  • Sisca Dwi Yarni Biomedical Analyst, Department of Biotechnology, Faculty of Medicine, Andalas University, Padang, Indonesia




Osteoarthritis, Mesenchymal stem cell Wharton’s jelly, MMP-1, TGF-β1


BACKGROUND: Mesenchymal Stem Cell Wharton’s Jelly (MSC-WJ) is promising candidates for osteoarthritis (OA) therapy since they have chondrogenic potential and the ability to form the extracellular matrix.

AIM: This study aimed to determine the effect of the time giving MSC-WJ on bioactive markers of osteoarthritis.

METHODS: The osteoarthritis rat model was treated by intra-articular injection with MSC-WJ and α _MEM as a control. Four and 8 weeks later performed a histological analysis of cartilage and the determination of the levels of Matrix Metalloproteinase-1(MMP-1) and Transforming growth factor β1 (TGF-β1) in serum by ELISA.

RESULTS: The results showed that administration of MSC-WJ showed improvement in the histological picture of knee joints in experimental animals characterized by an increase in cartilage thickness on the joint surface. The administration of MSC-WJ showed a tendency to decrease MMP-1 serum levels of OA rats treated for 8 weeks, although statistically did not show a significant difference. Whereas, administration of MSC-WJ showed a decrease in serum levels of TGF-β1 OA rat treated for 8 weeks.

CONCLUSION: MSC-WJ can repair damaged knee OA cartilage tissue. The administration of MSC-WJ can reduce serum levels of TGF-β1 OA rats treated for 8 weeks.


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How to Cite

Endrinaldi E, Ali H, Tofrizal T, Asterina A, Elmatris E, Yarni SD. Optimization of the Duration of the Administration of Mesenchymal Stem Cells Wharton’s Jelly to the Level of Matrix Metalloproteinase-1 and Transforming Growth Factor-β in Osteoarthritis Rat Model. Open Access Maced J Med Sci [Internet]. 2022 Mar. 9 [cited 2023 Dec. 6];10(A):563-71. Available from: https://oamjms.eu/index.php/mjms/article/view/8838

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