The Hepatoprotective Effect of Curcuma longa Extract on Dengue Virus Serotype-2 infected BALB/c Mice

Ni Wayan Anantika Riani; I Made Susila  Utama; Ida Bagus Oka  Winaya

doi:10.3889/oamjms.2023.8993

Authors

Ni Wayan Anantika Riani Department of Internal Medicine, Udayana University, Sanglah General Hospital, Bali, Indonesia https://orcid.org/0000-0002-6208-0742
I Made Susila Utama Tropical and Infectious Disease Division, Department of Internal Medicine, Udayana University, Sanglah General Hospital, Bali, Indonesia
Ida Bagus Oka Winaya Laboratory of Pathology, Faculty of Veterinary Medicine, Udayana University, Bali, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2023.8993

Keywords:

Alanine transaminase, Curcuma longa, Dengue, Liver, Pathology

Abstract

BACKGROUND: Dengue infection has a wide clinical spectrum ranging from asymptomatic infection to severe form with organ damage. Liver is among the common organ affected. Curcuma longa is a widely used herb that exhibits a hepatoprotective effect.

AIM: This study aimed to investigate effect of C. longa on liver necroinflammatory activity and alanine aminotransferase (ALT) levels in dengue virus serotype-2 (DENV-2) infected BALB/c mice.

MATERIALS AND METHODS: BALB/c mice were assigned into Group 1 (healthy mice), Group 2 (without C. longa administration), and Group 3 (C. longa administered). DENV-2 was inoculated intraperitoneally in Groups 2 and 3. C. longa extract was given at dose of 0.147 mg/mL intraorally in the 1st to 7th day. Liver histopathology and ALT level were assessed on the 7th day. Necroinflammatory activity was assessed with a Knodell histology activity index (HAI) score.

RESULTS: The median Knodell HAI score in Group 1, Group 2, and Group 3 were 1.0 (0–5), 7.0 (4–10), and 3.5 (0–8), respectively. The mean difference of Knodell HAI score between Group 2 and Group 3 is 4.1 (95% CI 1.75–6.45, p = 0.002). The mean ALT in Groups 1, 2, and 3 were 58.22 ± 18.31 IU/L, 58.26 ± 18.31 IU/L, and 57.00 ± 10.71 IU/L, respectively. One-way analysis of variance test showed no significant differences in ALT level levels among the three groups (F = 0.26, p = 0.974).

CONCLUSION: This study showed the hepatoprotective effect of C. longa that might decrease the liver necroinflammatory activity in dengue infection.

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