Moringa oleifera Prevents In vivo Carbon Tetrachloride-Induced Liver Fibrosis through Targeting Hepatic Stellate Cells

Supriono Supriono; Handono Kalim; Nur Permatasari; Hani Susianti

doi:10.3889/oamjms.2022.9119

Authors

Supriono Supriono Department of Internal Medicine, Gastroenterohepatology Division, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar Hospital, Malang, Indonesia https://orcid.org/0000-0002-6368-3983
Handono Kalim Department of Internal Medicine, Rheumatology Division, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar Hospital, Malang, Indonesia
Nur Permatasari Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
Hani Susianti Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar Hospital, Malang, Indonesia

DOI:

https://doi.org/10.3889/oamjms.2022.9119

Keywords:

Activation hepatic stellate cells, Apoptosis, Extrinsic pathway, Liver fibrosis, Moringa oleifera

Abstract

BACKGROUND: Moringa oleifera (MO) exhibits hepatoprotective properties and provides an anti-liver fibrosis effect. However, its mechanism related to the anti-liver fibrosis effect was still unclear.

AIM: The objective of this study was to explain the mechanism of liver fibrosis prevention by MO through hepatic stellate cells (HSCs).

MATERIALS AND METHODS: The liver fibrosis model was induced by the intraperitoneal injection of 10% CCl4 twice a week at a one cc/kg BW dose for 12 weeks and followed by a quantity of 2 cc/kg BW for the past 2 weeks. Ethanol extract of MO leaves (150, 300, and 600 mg/kg) was orally administered daily. Double immunofluorescence staining and terminal deoxynucleotidyl transferase dUTP nick end labeling analysis were applied to analyze the markers involved in HSCs activation and a-HSC apoptosis.

RESULTS: The results showed that the administration of MO could reduce transforming growth factor-β and nuclear factor-kappa B (NFκB), increase the expression of tumor necrosis factor-related apoptosis-inducing ligand-receptor 2 and caspase-3, and increase the number of apoptosis a-HSCs.

CONCLUSION: This study showed that the ethanol extract of MO leaves could inhibit liver fibrosis by inhibiting HSCs activation and inducing of a-HSCs apoptosis through the extrinsic pathway.

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