Anxiolytic Activity of Ethanolic Extract of Three Species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, and Zingiber americans)


  • Widyastiwi Widyastiwi Department of Pharmacy, Poltekkes Kemenkes Bandung, West Java, Indonesia; Center of Excellence, Poltekkes Kemenkes Bandung, West Java, Indonesia
  • Roseno Department of Pharmacy, Poltekkes Kemenkes Bandung, West Java, Indonesia



Anxiolytic, Zingiberaceae, Elevated plus maze, Open field test, Holeboard test


BACKGROUND: Lempuyang, or widely known as wild ginger, has been used in Indonesia as traditional medicine for a long time. However, scientific research supporting its medicinal use is still very limited.

AIM: This research was conducted to investigate the anxiolytic activity of three species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, and Zingiber americans).

METHODS: Sixty-six male Swiss Webster mice were divided into 11 groups (negative control, positive control, and experiment groups consisting of low, moderate, and high dose of Z. zerumbet, Z. aromaticum, and Z. americans extract, respectively). Anxiolytic activity was evaluated by three methods: elevated plus maze test, open field test, and hole board test.

RESULTS: The results showed that these three species of Indonesian Lempuyang lowered the anxiety response in elevated plus maze test, open field test, and hole board test compared to negative control (p < 0.05). The best anxiolytic activity was achieved by moderate dose of Z. americans (8.5 mg/20 gbb), consistently through three methods applied.

CONCLUSION: This research supports the potential use of the ethanolic extract of Lempuyang (Z. zerumbet, Z. aromaticum, and Z. americans) as a complementary therapy for anxiety.


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DeBattista C. Antidepressant agents. In: Katzung BG, editor. Basic & Clinical Pharmacology. 14th ed. New York: McGraw-Hill Education; 2018. p. 532-52.

Indonesian Ministry of Health. 2018 Basic Health Research. Jakarta: Ministry of Health; 2018.

Silalahi M. Botany and bioactivity of lempuyang (Zingiber zerumbet (L.) Smith.). J Pendidikan Matematika Sains. 2018;2(2):147-60.

Riyanto S. Identification of the isolated compounds from Zingiber amaricans BL. Rhizome. Indones J Chem. 2010;7(1):93-6. DOI:

Sutardi N, Wientarsih L, Handharyani IE, Setiyono A. Indonesian wild ginger (Zingiber sp) extract: Antibacterial activity against Mycoplasma gallisepticum. IOSR J Pharm. 2015;5(10):59-64.

Lim TK. Edible Medicinal and Non-Medicinal Plants. Vol. 12. Modified Stems, Roots, Bulbs, London: Springer; 2016. p. 1-659. DOI:

Jafarian S, Ling KH, Hassan Z, Perimal-Lewis L, Sulaiman MR, Perimal EK. Effect of zerumbone on scopolamine-induced memory impairment and anxiety-like behaviours in rats. Alzheimer’s Dement. 2019;(5):637-43. PMid:31687471 DOI:

Seibenhener ML, Wooten MC. Use of the open field maze to measure locomotor and anxiety-like behavior in mice. J Vis Exp. 2015;(96):e52434. PMid:25742564 DOI:

Kelley AE. Locomotor activity and exploration. In: van Haaren F, editor. Methods in Behavioral Pharmacology. Handbook of Behavioral Neuroscience. Vol. 10. USA: Elsevier; 1993. p. 499-518. DOI:

Brown GR, Nemes C. The exploratory behaviour of rats in the hole-board apparatus: Is head-dipping a valid measure of neophilia? Behav Processes. 2008;78(3):442-8. PMid:18406075 DOI:

File SE, Wardill AG. The reliability of the hole-board apparatus. Psychopharmacologia. 1975;44(1):47-51. PMid:1197579 DOI:

Anas Y, Puspitasari N, Nuria MC. Stimulatory activity of ethanolic extract of clove flower (Syzgium aromaticum L.) in Swiss Webster and its active component identification. J Biol. 2019;12(2):13-22.

Shahed-Al-Mahmud M, Lina SM. Evaluation of sedative and anxiolytic activities of methanol extract of leaves of Persicaria hydropiper in mice. Clin Phytosci. 2017;3(1):p 1-12. DOI:

Trevor AJ. Sedative-hypnotic drugs. In: Bertram GK, editor. Basic & Clinical Pharmacology. 14th ed. New York: McGraw-Hill Education; 2018. p. 381-95.

Liao YJ, Zhai HF, Zhang B, Duan TX, Huang JM. Anxiolytic and sedative effects of dehydroeffusol from Juncus effusus in mice. Planta Med. 2011;77(5):416-20. PMid:21104609 DOI:

Campos AC, Fogaça MV, Aguiar DC, Guimarães FS. Animal models of anxiety disorders and stress. Rev Bras Psiquiatria. 2013;35(Suppl 2):101-11. DOI:

Dhar AS, Rahman M, Rafe MR, Molla MI. Assessment of antidepressant and sedative-hypnotic activities of methanolic crude extracts of Stephania japonica (Thunb.) Miers. whole plants. Curr Issues Pharm Med Sci. 2020;33(1):51-5. DOI:

Tatem KS, Quinn JL, Phadke A, Yu Q, Gordish-Dressman H, Nagaraju K. Behavioral and locomotor measurements using an open field activity monitoring system for skeletal muscle diseases. J Vis Exp. 2014;(91):51785. PMid:25286313 DOI:

Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: A review. Eur J Pharmacol. 2003;463:3-33. PMid:12600700 DOI:

Schneider P, Ho YJ, Spanagel R, Pawlak CR. A novel elevated plus-maze procedure to avoid the one-trial tolerance problem. Front Behav Neurosci. 2011;5(43):1-8. PMid:21845176 DOI:

Biedermann SV, Biedermann DG, Wenzlaff F, Kurjak T, Nouri S, Auer MK, et al. An elevated plus-maze in mixed reality for studying human anxiety-related behavior. BMC Biol. 2017;15(1):125. PMid:29268740 DOI:

Yob NJ, Zakaria ZA, Jofrry SM, Affandi MM, Teh LK, Salleh MZ. Zingiber zerumbet (L.) Smith: A review of its ethnomedicinal, chemical, and pharmacological uses. Evid Based Complement Alternat Med. 2011;2011:543216. DOI:

Sulaiman MR, Tengku Mohamad TA, Shaik Mossadeq WM, Moin S, Yusof M, Mokhtar AF, et al. Antinociceptive activity of the essential oil of Zingiber zerumbet. Planta Med. 2010;76(2):107-12. PMid:19637111 DOI:

Ogawa K, Miyoshi T, Kitayama T, Ito M. Locomotor-reducing effects and structural characteristics of inhaled zerumbone and tetrahydrozerumbone derivatives. Biol Pharm Bull. 2014;37(9):1559-63. PMid:25177038 DOI:

Bahi A, Al Mansouri S, Al Memari E, Al Ameri M, Nurulain SM, Ojha S. β-Caryophyllene, a CB2 receptor agonist produces multiple behavioral changes relevant to anxiety and depression in mice. Physiol Behav. 2014;135:119-24. PMid:24930711 DOI:

Satou T, Murakami S, Matsuura M, Hayashi S, Koike K. Anxiolytic effect and tissue distribution of inhaled Alpinia zerumbet essential oil in mice. Nat Prod Commun. 2010;5(1):143-6. PMid:20184040 DOI:

Koga AY, Beltrame FL, Pereira AV. Several aspects of Zingiber zerumbet: A review. Rev Bras Farmacognosia. 2016;26(3):385-91. DOI:

Sloley BD, Urichuk LJ, Morley P, Durkin J, Shan JJ, Pang PK, et al. Identification of kaempferol as a monoamine oxidase inhibitor and potential neuroprotectant in extracts of Ginkgo biloba Leaves. J Pharm Pharmacol. 2000;52(4):451-9. PMid:10813558 DOI:

Calderón-Montaño JM, Burgos-Morón E, Pérez-Guerrero C, López-Lázaro M. A review on the dietary flavonoid kaempfero. Mini Rev Med Chem. 2011;11(4):298-344. PMid:21428901 DOI:

Muhlasin A. Chromatogram Profile of Lempuyang Emprit (Zingiber amaricans Bl.) Ethanolic Extract and Determination of Zerumbon Content by High Performance Liquid Chromatography. [Thesis]. Surakarta: Universitas Muhammadiyah Surakarta; 2013.

Mohammad N, Akhtar Y, Jantan I, Arshad L, Haque A. standardized ethanol extract, essential oil and zerumbone of Zingiber zerumbet rhizome suppress phagocytic activity of human neutrophils. BMC Complement Med. 2019;5:331. PMid:31752812 DOI:

Cid-Ortega S, Monroy-Rivera JA. Extraction of kaempferol and its glycosides using supercritical fluids from plant sources: A review. Food Technol Biotechnol. 2018;56(4):480-93. PMid:30923445 DOI:

Indonesian Ministry of Health. Materia Medika Indonesia. II. Jakarta: Indonesian Ministry of Health; 1978. p. 101-27.

Calabrese EJ, Mattson MP. How does hormesis impact biology, toxicology, and medicine? NPJ Aging Mech Dis. 2017;3(1):13. PMid:28944077 DOI:

Calabrese EJ. An assessment of anxiolytic drug screening tests: Hormetic dose responses predominate. Crit Rev Toxicol. 2008;38:489-542. PMid:18615308 DOI:

Chang CJ, Tzeng TF, Liou SS, Chang YS, Liu IM. Acute and 28-day subchronic oral toxicity of an ethanol extract of Zingiber zerumbet (L.) Smith in rodents. Evid Based Complement Alternat Med. 2012;2012:608284. PMid:22536288 DOI:




How to Cite

Widyastiwi W, Roseno M. Anxiolytic Activity of Ethanolic Extract of Three Species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, and Zingiber americans). Open Access Maced J Med Sci [Internet]. 2022 Apr. 7 [cited 2023 Mar. 31];10(A):695-701. Available from: