The Effectiveness of Curcumin on Transforming Growth Factor-β and the Proportion of Liver Fibrosis in Deutschland Denken Yoken Mice with Common Bile Duct Ligation

Authors

  • Prathita Amanda Department of General Surgery, Faculty of Medicine, Universitas Diponegoro, Dr. Kariadi General Hospital, Semarang, Indonesia
  • Erik Prabowo Department of Digestive Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
  • Muflihatul Muniroh Department of Physiology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
  • Abdul Mughni Rozy Department of Digestive Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, IndonesiaDepartment of Digestive Surgery, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
  • Neni Susilaningsih Department of Anatomy Histology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia https://orcid.org/0000-0003-1960-507X

DOI:

https://doi.org/10.3889/oamjms.2022.9152

Keywords:

Curcumin, Ursodeoxycholic acid, Common bile duct ligation, Liver fibrosis

Abstract

ABSTRACT

 

Background/Aims: Curcumin has been associated to reduce liver fibrosis damage. For this purpose, TGF-b and proportion of liver fibrosis were assessed.

Methods: A total of 75 male DDY mice models were included in 15 treatment groups. The treatment and positive control groups were given curcumin, UDCA, and a combination of curcumin and UDCA. Common bile duct ligation was performed 7 days after the treatment following the histopathology examination of the liver on days 7,14, and 21.

Results: Curcumin decreased the expression of TGF-β compared to the negative control (p<.001). It also reduces the expression of TGF-β compared to UDCA but without any significant differences (p = 0,755; p = 0,701; p=0,677), and combination group (p=0,204; p=0,549; p=0,276). Curcumin also reduces the proportion of liver fibrosis compared to UDCA but without any significant differences (p=0,825; p=0,199; p=0,915), and combination group (p=0,859, p=0,328, p=0,076).

Conclusions: Curcumin has the ability on reducing the expression of TGF-β and the proportion of liver fibrosis. It can be suggested that curcumin almost has the same effectiveness as UDCA.

Keywords: Curcumin, ursodeoxycholic acid, common bile duct ligation, liver fibrosis

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References

Weiskirchen R, Weiskirchen S, Tacke F. Recent advances in understanding liver fibrosis: Bridging basic science and individualized treatment concepts. F1000Res. 2018;7:F1000 Faculty Rev-921. https://doi.org/10.12688/f1000research.14841.1 PMid:30002817 DOI: https://doi.org/10.12688/f1000research.14841.1

Coban S, Yildiz F, Terzi A, Al B, Aksoy N, Bitiren M, et al. The effects of Nigella sativa on bile duct ligation induced-liver injury in mice. Cell Biochem Funct. 2010;28(1):83-8. https://doi.org/10.1002/cbf.1624 PMid:20029957 DOI: https://doi.org/10.1002/cbf.1624

Chen N, Geng Q, Zheng J, He S, Huo X, Sun X. Suppression of the TGF-β/Smad signaling pathway and inhibition of hepatic stellate cell proliferation play a role in the hepatoprotective effects of curcumin against alcohol-induced hepatic fibrosis. Int J Mol Med. 2014;34(4):1110-6. https://doi.org/10.3892/ijmm.2014.1867 PMid:25069637 DOI: https://doi.org/10.3892/ijmm.2014.1867

El-Meguid MA, Dawood RM, Mokhles MA, El Awady MK. Extrahepatic upregulation of transforming growth factor beta 2 in HCV genotype 4-induced liver fibrosis. J Interferon Cytokine Res. 2018;38(8):341-7. https://doi.org/10.1089/jir.2018.0045 PMid:30130153 DOI: https://doi.org/10.1089/jir.2018.0045

Derynck R, Budi EH. Spesificity, versatility, and control of TGF beta family signaling. Sci Signal. 2019;12(570):eaav5183. https://doi.org/10.1126/scisignal.aav5183 PMid:30808818 DOI: https://doi.org/10.1126/scisignal.aav5183

Rivera-Espinoza Y, Muriel P. Pharmacological actions of curcumin in liver diseases or damage. Liver Int. 2009;29(10):1457-66. https://doi.org/10.1111/j.1478-3231.2009.02086.x PMid:19811613 DOI: https://doi.org/10.1111/j.1478-3231.2009.02086.x

Farzaei MH, Zobeiri M, Parvizi F, El-Senduny FF, Marmouzi I, Coy-Barrera E, et al. Curcumin in liver diseases: A systematic review of the cellular mechanisms of oxidative stress and clinical perspective. Nutrients. 2018;10(7):855. https://doi.org/10.3390/nu10070855 PMid:29966389 DOI: https://doi.org/10.3390/nu10070855

Supriono S, Pratomo B, Praja DI. The effects of curcumin on NF-κB level and degree of liver fibrosis in rat liver fibrosis. J Penyakit Dalam Indones. 2019;5(4):174-83. https://doi.org/10.7454/jpdi.v5i4.271 DOI: https://doi.org/10.7454/jpdi.v5i4.271

Tang N, Zhang Y, Liang Q, Liu Z, Shi Y. The role of ursodeoxycholic acid on cholestatic hepatic fibrosis in infant mice. Mol Med Rep. 2018;17(3):3837-44. https://doi.org/10.3892/mmr.2017.8284 PMid:29257337 DOI: https://doi.org/10.3892/mmr.2017.8284

Milani M. Ursodeoxycholic acid (UDCA) in biliary diseases: A Clinical review. Br J Med Med Res. 2014;4(9):1783-90. https://doi.org/10.9734/bjmmr/2014/7186 DOI: https://doi.org/10.9734/BJMMR/2014/7186

Tag CG, Sauer-Lehnen S, Weiskirchen S, Borkham-Kamphorst E, Tolba RH, Tacke F, et al. Bile duct ligation in mice: Induction of inflammatory liver injury and fibrosis by obstructive cholestasis. J Vis Exp. 2015;96:52438. https://doi.org/10.3791/52438 PMid:25741630 DOI: https://doi.org/10.3791/52438

Supriono S, Nugraheni A, Kalim H, Eko MH. The effect of curcumin on regression of liver fibrosis through decreased expression of transforming growth factor-β1 (TGF-β1). Indones Biomed J. 2019;11(1):52-8. https://doi.org/10.18585/inabj.v11i1.463 DOI: https://doi.org/10.18585/inabj.v11i1.463

Guan S, Yu P, Cao J, Xi X, Zhang Q, Zhu C et al. Ginsenoside Rg1 protects against cigarette smoke-induced airway remodeling by suppressing the TGF-β1/Smad3 signaling pathway. Am J Transl Res. 2020;12(2):493-506. PMid:32194898

Fabregat I, Moreno-Càceres J, Sánchez A, Dooley S, Dewidar B, Giannelli G, et al. TGF-signalling and liver disease. FEBS J. 2016;283(12):2219-32. https://doi.org/10.1111/febs.13665 PMid:26807763 DOI: https://doi.org/10.1111/febs.13665

Putra R, Handaya AY, Barmawi A. Effectivity of glutathione and curcumin in hepatic fibrosis induced by cholestasis in Wistar mice. 2021;12(2):459-62. https://doi.org/10.15562/ism.v12i2.997 DOI: https://doi.org/10.15562/ism.v12i2.997

Gheibi S, Ghaleh HE, Motlagh BM, Azarbayjani AF, Zarei L. Therapeutic effects of curcumin and ursodexycholic acid on non-alcoholic fatty liver disease. Biomed Pharmacother. 2019;115:108938. https://doi.org/10.1016/j.biopha.2019.108938 PMid:31071511 DOI: https://doi.org/10.1016/j.biopha.2019.108938

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Published

2022-06-10

How to Cite

1.
Amanda P, Prabowo E, Muniroh M, Rozy AM, Susilaningsih N. The Effectiveness of Curcumin on Transforming Growth Factor-β and the Proportion of Liver Fibrosis in Deutschland Denken Yoken Mice with Common Bile Duct Ligation. Open Access Maced J Med Sci [Internet]. 2022 Jun. 10 [cited 2024 Mar. 29];10(A):1157-63. Available from: https://oamjms.eu/index.php/mjms/article/view/9152