Evaluation of IGF-1, TNF-α, and TGF-β Gene Expression after Oral Vitamin D Supplementation in School-Aged Children with Chronic Bronchial Asthma

Authors

  • Abeer Ramadan Department of Molecular Genetics and Enzymology, Human Genetics and Genomic Research Institute, National Research Center, Giza, Egypt https://orcid.org/0000-0001-5757-1769
  • Sara Sallam Department of Child Health, Medical Research and Clinical Studies Institute, National Research Center, Giza, Egypt
  • Rasha Yousef Department of Clinical and Chemical Pathology, Medical Research and Clinical Studies Institute, National Research Center, Giza, Egypt
  • Mai Elsheikh Department of Complementary Medicine, Medical Research and Clinical Studies Institute, National Research Center, Giza, Egypt
  • Asmaa Ali Department of Pulmonary Medicine, Abbassia Chest Hospital, Ministry of Health, Cairo, Egypt
  • Yasmine Elhusseny Department of Biochemistry and Molecular Biology, New Giza University, Giza, Egypt
  • Sally Ishak Department of Pediatric, Faculty of Medicine, Ain Shams University Hospital, Cairo, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.9266

Keywords:

Vitamin D, Bronchial asthma, TNF-α, IGF-1, TGF-β, Gene expression

Abstract

BACKGROUND: Airway remodeling in children with bronchial asthma is due to the effect of inflammatory mediators and growth factors on the bronchial epithelium. Vitamin D (VitD) has immunomodulatory effect in many inflammatory diseases as bronchial asthma. The ant-inflammatory and anti-fibrotic role of VitD could prevent or improve air way remodeling in asthmatic patients.

AIM: The study investigated the effect of VitD supplementation on the expression of transforming growth factor-beta (TGF-β), tumor necrosis factor-alpha (TNF-α), and insulin growth factor 1(IGF-1) and to correlate them with asthma severity and level of control.

METHODS: The serum level of VitD and the mRNA expression of IGF-1, TGF-β, and TNF-α were estimated in 50 patients and 20 healthy controls control subjects using quantitative PCR in real-time. Asthmatic patients with VitD deficiency received VitD supplementation for 2 months followed by remeasurement of serum VitD and the genes expression TGF-β, TNF-α, and IGF-1.

RESULT: Pre-intake of VitD and serum level of VitD were lower in all patients than control subjects (p = 0.005). VitD level was directly correlated with IGF-1 mRNA expression, which was indirectly correlated with TGF-β, r = 0.5 and −0.57; p = 0.0001 and 0.002, respectively. After VitD supplementation, the expression of the TGF-β mRNA gene was the only gene that decreased significantly (p = 0.04) together with improved asthma control and spirometric parameters.

CONCLUSIONS: VitD supplementation down regulated the gene expression of TGF-β and improved asthma control level, but it did not significantly affect the gene expression of TNF-α and IGF-1.

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2022-05-19

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1.
Ramadan A, Sallam S, Yousef R, Elsheikh M, Ali A, Elhusseny Y, Ishak S. Evaluation of IGF-1, TNF-α, and TGF-β Gene Expression after Oral Vitamin D Supplementation in School-Aged Children with Chronic Bronchial Asthma. Open Access Maced J Med Sci [Internet]. 2022 May 19 [cited 2024 Mar. 29];10(B):1358-64. Available from: https://oamjms.eu/index.php/mjms/article/view/9266

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