Immunohistochemical Study of Cancer Stem Cell marker, Tight Junction Protein, and Lymphatic Density in Malignant Salivary Gland Tumors

Marwa Maghrabi; Doaa A. Farag; Marwa Shakweer; Mohamed Negm; Nadia A. El-Sissy

doi:10.3889/oamjms.2022.9305

Authors

Marwa Maghrabi Department of Oral Pathology, Faculty of Dentistry, University of Tripoli, Tripoli, Libya
Doaa A. Farag Department of Oral and Maxillofacial Pathology, Faculty of Oral and Dental Medicine, Mansoura University, Mansoura, Egypt
Marwa Shakweer Department of Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt
Mohamed Negm Department of Pathology, Faculty of Medicine, Cairo University, Giza, Egypt https://orcid.org/0000-0002-6889-8086
Nadia A. El-Sissy Department of Oral and Maxillofacial Pathology, Faculty of Oral and Dental Medicine, Mansoura University, Mansoura, Egypt

DOI:

https://doi.org/10.3889/oamjms.2022.9305

Keywords:

Malignant salivary gland tumors, c-kit (CD117), Claudin-1, Podoplanin

Abstract

Background: CD117/c-kit, is a powerful stem cell marker for malignant salivary gland tumors in which dysregulation of c-kit is closely associated with impairment of cell adhesion molecules and cancer metastasis.

Objective: The main purpose of this work is to evaluate the immunohistochemical expression of c-kit, and claudin-1 and measure the density of lymph vessels (LVD) in common malignant salivary gland tumors by using podoplanin (D2-40) antibody.

Materials and Methods: Immunohistochemical staining with streptavidin peroxidase was used to analysis the expression of c-kit, claudin-1 and stained podoplanin (D2-40) lymphatic vessels on fifty archival paraffin blocks of malignant salivary gland tumor (MSGTs) cases included 20 cases of AdCC, 11 cases of MEC, 10 cases of CXPA, 6 cases of AcCC, and 3 cases of PAC.

Results: The immunopositivity of c-kit (CD117) was detected in 44/50 (88%) of studied cases, whereas, claudin-1 protein was observed in 35 (70%) of our specimens of MSGTs. Count down of stained lymph vessels between examined cases was, MEC on the top, followed by CXPA, AdCC, PAC and AcCC. A direct correlation was observed between c-kit and lymphatic density, on the other hand, the inverse correlation was found d between c-kit and cld-1, as well as, between cld-1 and lymphatic density

Conclusion: Up regulation of cancer stem cell marker c-kit (CD117) expression is associated with decrease of tight junction protein cld-1 and increase the density of stained lymphatic vessels by podoplanin (D2-40) antibody which confirms the using of c-kit inhibitor to improve treatment strategy of malignant salivary gland tumors.

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